Structure of PDB 7vsa Chain B Binding Site BS01

Receptor Information

>7vsa Chain B (length=155) Species: 83333 (Escherichia coli K-12)

MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELM
AAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKN
VDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTG
YQVEV

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 7vsa Chain B Residue 201

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7vsa Pivotal role of a conserved histidine in Escherichia coli ribonuclease HI as proposed by X-ray crystallography.
• Resolution: 1.76 Å
• Binding residue (original residue number in PDB): D10 E48
• Binding residue (residue number reindexed from 1): D10 E48
• Annotation score: 4

Enzymatic activity

• Enzyme Commision number: 3.1.26.4: ribonuclease H.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003676 nucleic acid binding
• GO:0004519 endonuclease activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity
• GO:0005515 protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006401 RNA catabolic process
• GO:0043137 DNA replication, removal of RNA primer

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7vsa, PDBe:7vsa, PDBj:7vsa
• PDBsum: 7vsa
• PubMed: 35234152
• UniProt: P0A7Y4|RNH_ECOLI Ribonuclease HI (Gene Name=rnhA)