Structure of PDB 7mjw Chain B Binding Site BS01

Receptor Information

>7mjw Chain B (length=441) Species: 820 (Bacteroides uniformis)

NKKLFIETYGCQMNVADSEVIASVMQMAGYSVADTLEEADAVFMNTCSIR
DNAEQKILNRLEFFHSLKKKKRGLIVGVLGCMAERVKDDLITNHHVDLVV
GPDAYLTLPELIASVEAGEKAMNVELSTTETYRDVIPSRICGNHISGFVS
IMRGCNNFCTYCIVPYTRGRERSRDVESILNEVADLVAKGYKEVTLLGQN
VNSYRFEKPDGETITFPMLLRTVAEAAPGVRIRFTTSHPKDMSDETLQVI
ADMPNVCKHIHLPVQSGSSRILKLMNRKYDREWYMDRVAAIRRIIPDCGL
STDIFSGFHSETEEDHQLSLSLMEECGYDSAFMFKYSERPGTHASKHLPD
DVPEEVKIRRLNEIIALQNRLSAEANARCVGKTYEVLVEGVSKRSRDQLF
GRTEQNRVVVFDRGTHRVGDFVMVKVTESSSATLKGEEVAG

Ligand information

>7mjw Chain G (length=13)

ggacugaaaaucc

<<<.......>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mjw Structural basis for tRNA methylthiolation by the radical SAM enzyme MiaB.
• Resolution: 1.4 Å
• Binding residue (original residue number in PDB): G26 C27 Q28 C63 R66 N68 K72 T252 D319 F348 M349 F350 I381 K409 R410 R418 V424 V426 S446 S447 A448 T449 K451
• Binding residue (residue number reindexed from 1): G10 C11 Q12 C47 R50 N52 K56 T236 D303 F332 M333 F334 I365 K393 R394 R402 V408 V410 S430 S431 A432 T433 K435

Enzymatic activity

• Enzyme Commision number: 2.8.4.3: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016740 transferase activity
• GO:0035596 methylthiotransferase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051538 3 iron, 4 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006400 tRNA modification
• GO:0008033 tRNA processing
• GO:0035600 tRNA methylthiolation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7mjw, PDBe:7mjw, PDBj:7mjw
• PDBsum: 7mjw
• PubMed: 34526715
• UniProt: A0A174NUT3