Structure of PDB 7mi9 Chain B Binding Site BS01

Receptor Information

>7mi9 Chain B (length=338) Species: 243231 (Geobacter sulfurreducens PCA)

GLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLGETSQVALFGNATLTT
AALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVETRTYQYQRSFDPETC
LNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPGLLMSLQDDMRHAMRA
PSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGFDFTTRNRRPPKDPVN
ALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRFGRPALALDMMEPFRP
LIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSARKRFIAGFERRMEQEV
THPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPNFVT

Ligand information

>7mi9 Chain G (length=80)

aggacaacgttacggacggcacagcctttttgcttcaatgaggccggggc
atcatggccccggaatacggctcttttccg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mi9 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Resolution: 3.89 Å
• Binding residue (original residue number in PDB): P229 K230 Y232 E244 E245 R246 N265 T267 E374 V375 G378 I379 F455
• Binding residue (residue number reindexed from 1): P9 K10 Y12 E24 E25 R26 N45 T47 E154 V155 G158 I159 F235

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mi9, PDBe:7mi9, PDBj:7mi9
• PDBsum: 7mi9
• PubMed: 34588691
• UniProt: Q74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)