Structure of PDB 7mei Chain B Binding Site BS01

Receptor Information
>7mei Chain B (length=1166) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
DESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTL
ILEQLAQHTTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARL
RNLTYSSGLFVDVKTYEAIDVPGRELKYEDSESGKVFIGRLPIMLRSKNC
YLSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQERSAGNIVQVFKKA
APSPISHVAEIRSALEKGSRFISTLQVKLYGRSSARTIKATLPYIKQDIP
IVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGFVIQDRETAL
DFIGRRGTALGIKKEKRIQYAKDILQKEFLPHITQLEGFESRKAFFLGYM
INRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRY
MQRTVEEAHDFNMKLAINAKTITSGLKYALATGNWGEQKKAMSSRAGVSQ
VLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQ
ACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPLEDYVPHQSPDATRVF
VNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKELKIFTDAG
RVYRPLFIVEDDESLGHKELKVRKGHIAKLMATEYQDEYTWSSLLNEGLV
EYIDAEEEESILIAMQPEDLEPAAKRIRATTFTHCEIHPSMILGVAASII
PFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLGTT
RAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFF
RSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVS
GEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTT
NQDGLKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGI
VPDLIINPHAIPSRMTVAHLIECLLSKVAALSGNEGDASPFTDITVEGIS
KLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHAR
ARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLMEASD
AFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQE
LMAMNIPRLYTDRSRD
Ligand information
>7mei Chain N (length=74) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cactagtgcctaaaaaaaatttatagtgcaaaaaaaccaaaaaaaaaaat
tctccttcgagtgcttatcggtaa
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7mei Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II.
Resolution3.54 Å
Binding residue
(original residue number in PDB)		R241 S248 I251 Y275 K426 I502 G867 M868 S869
Binding residue
(residue number reindexed from 1)		R212 S219 I222 Y244 K395 I471 G811 M812 S813
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Gene Ontology
Molecular Function
GO:0001055 RNA polymerase II activity
GO:0003677 DNA binding
GO:0003729 mRNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0003968 RNA-dependent RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0001172 RNA-templated transcription
GO:0006351 DNA-templated transcription
GO:0006366 transcription by RNA polymerase II
GO:0006367 transcription initiation at RNA polymerase II promoter
GO:0006368 transcription elongation by RNA polymerase II
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005665 RNA polymerase II, core complex
GO:0005739 mitochondrion
GO:0010494 cytoplasmic stress granule

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Molecular Function
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Biological Process
View graph for
Cellular Component
External links
PDB RCSB:7mei, PDBe:7mei, PDBj:7mei
PDBsum7mei
PubMed35051353
UniProtP08518|RPB2_YEAST DNA-directed RNA polymerase II subunit RPB2 (Gene Name=RPB2)

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