Structure of PDB 7a0r Chain B Binding Site BS01

Receptor Information

>7a0r Chain B (length=206) Species: 243230 (Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)

MKGILGTKIGMTQIWKNDRAIPVTVVLAGPCPIVQRKTAQTDGYEAVQIG
YAPKAERKVNKPMQGHFAKAGVAPTRILREFRGFAPDGDSVNVDIFAEGE
KIDATGTSKGKGTQGVMKRWNFAGGPASHGSKKWHRRPGSIGQRKTPGRV
YKGKRMAGHMGMERVTVQNLEVVEIRAGENLILVKGAIPGANGGLVVLRS
AAKASA

Ligand information

>7a0r Chain X (length=2730)

ggucaagauaguaaggguccacgguggaugcccuggcgcuggagccgaug
aaggacgcgauuaccugcgaaaagccccgacgagcuggagauacgcuuug
acucggggauguccgaauggggaaacccaccucguaagagguauccgcaa
ggaugggaacucagggaacugaaacaucucaguaccugaaggagaagaaa
gagaauucgauuccguuaguagcggcgagcgaacccggaucagcccaaac
cgaaacgcuugcguuucgggguuguaggaccaucaagccgaaguggcugg
aaagcuacaccucagaaggugagaguccuguaggcgaacgagccugagua
ggucguuguucgugaaacgaugacugaauccgcgcggaccaccgcgcaag
gcuaaauacucccagugaccgauagcgcauaguaccgugagggaaaggug
aaaagaaccccgggaggggagugaaagagaaccugaaaccguggacuuac
aagcagucauggcaccuuaugcguguuauggcgugccuauugaagcauga
gccggcgacuuagaccugacgugcgagcuuaaguugaaaaacggaggcgg
agcgaaagcgaguccgaauagggcggcauuaguacgucgggcuagacucg
aaaccaggugagcuaagcaugaccagguugaaacccccgugacagggggc
ggaggaccgaaccggugccugcugaaacagucucggaugaguuguguuua
ggagugaaaagcuaaccgaaccuggagauagcuaguucuccccgaaaugu
auugagguacagccucggauguugaccauguccuguagagcacucacaag
gcuaaaaccuuaugaaacuccgaaggggcacgcguuuaguccgggaguga
ggcugcgagagcuaacuuccguagccgagagggaaacaacccagaccauc
agcuaaggucccuaaaugaucgcucagugguuaaggaugugucgucgcau
agacagccaggagguuggcuuagaagcagccacccuucaaagagugcgua
auagcucacuggucgagugacgaugcgccgaaaaugaucggggcucaagu
gaucuaccgaagcuauggauucaacucgcgaagcgaguugucugguaggg
gagcguucaguccgcggagaagccauaccggaaggagugguggagccgac
ugaagugcggaugccggcaugaguaacgauaaaagaagugagaaucuucu
ucgccguaaggacaaggguuccuggggaagggucguccgcccagggaaag
ucgggaccuaaggugaggccgaacggcgcagccgauggacagcaggucaa
gauuccugcaccgaucauguggagugauggagggacgcauuacgcuaucc
aaugccaagcuauggcuaugcugguugguacgcucaagggcgaucggguc
agaaaaucuaccggucacaugccucagacguaucgggagcuuccucggaa
gcgaaguuggaaacgcgacggugccaagaaaagcuucuaaacguugaaac
augauugcccguaccgcaaaccgacacagguguccgagugucaaugcacu
aaggcgcgcgagagaacccucguuaaggaacuuugcaaucucaccccgua
acuucggaagaagggguccccacgcuucgcguggggcgcagugaauaggc
ccaggcgacuguuuaccaaaaucacagcacucugccaacacgaacagugg
acguauagggugugacgccugcccggugccggaaggucaaguggagcggu
gcaagcugcgaaaugaagccccggugaacggcggcuaagguagcgaaauu
ccuugucggguaaguuccgaccugcacgaaaggcguaacgaucugggcgc
ugucucaacgagggacucggugaaauugaauuggcuguaaagaugcggcc
uacccguagcaggacgaaaagaccccguggagcuuuacuauagucuggca
uugggauucggguuucuagaaacuuggauuucuaaccugaaaaaucacuu
ucggggaccgugcuuggcggguaguuugacuggggcggucgccucccaaa
auguaacggaggcgcccaaaggucaccucaagacgguuggaaaucgucug
uagagcgcaaagguagaagguggcuugacugcgagacugacacgucgagc
agggaggaaacucgggcuuagugaaccggugguaccguguggaagggcca
ucgaucaacggauaaaaguuaccccggggauaacaggcugaucucccccg
agaguccauaucggcggggagguuuggcaccucgaugucggcucgucgca
uccuggggcugaagaaggucccaaggguugggcuguucgcccauuaaagc
ggcacgcgagcuggguucagaacgucgugagacaguucggucucuauccg
cuacgggcgcaggagaauugaggggaguugcuccuaguacgagaggaccg
gagugaacggaccgcuggucucccugcugucguaccaacggcacaugcag
gguagcuauguccggaacggauaaccgcugaaagcaucuaagcgggaagc
cagccccaagaugaguucucccacuguuuaucagguaagacucccggaag
accaccggguuaagaggccaggcgugcacgcauagcaauguguucagcgg
acuggugcucaucagucgaggucuugacca

<<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<....
..<<<<<<.[...((.>>>.....<<<<<<...<<.))...]..>>....
..>>>>>>...>>>....<<<....>>><<<<<....>>>>><<<<....
>>>>>>...<<<<<<.....<......>......>>>>>.>.........
<<....>>...<<<<......<<.....>>.....>>>>...<<<....<
<<<<.<<....>>.>>>>>......<<<....<<..<<<...<<<<<...
...>>>>>((......<<<.....))>>>...>>>....>>.>>>.....
<<<<<<<<.........>>>>>>>>......<<<<<..((((>>>>>..>
>>......>>.>>>>>>.>>..............<<....>>....>>..
.......<<<<....>>>>..................>>>>>>>>>>>..
.....<<<<<<<<<<.......>>>>>>>>>>...<.<........>.>.
<<<<<<....<<<.<<<<<<<<<<..<<<...<<<....>>>...>>>..
.<<....>>...<<......>>.........>>>>>>>>>>>>>..<<.<
...<<<<<<.....<<<<<<<<<..<<<.....<<<<<......>>>>>.
.....>>>...<<<<.<.<<........>>.>>>>>....>>>>>>>>>.
..<<<.....>>>......>>>>>>......>.>>..<<<<<<....<<<
<<....>>>>><<<<<<<<<...<<<<.<<<<<<...<<<...<<<.<<<
<......>>>>.>>>..>>.>...>>>>>>.>.>>>..>>>>>>>.>>..
<<<<<<<.<<......>>.>>>>>>>....<<<......>>>...<<<<.
<<<...<<<(((.....<<<<<<...........<<..<<<<<<<<<...
...<.<<<<<.<<<..<<<<.......>>>>..>>>.........<<...
...>>...>>>>>.>..>>>>>>>>>.>>..........)))......>>
>>>>.>>>...>>>>>>><..<<<<<<<<...>>>>>>>>..>....>>>
>>><.<<<<<<<.<<......<<<<<.<<....>>.>>>>>...>>.>>>
>>>>..>.....>>>>>><......<<.....<<<<<<.......>>>>>
><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....(
(<<<<.<<...<<<...<<<<..>>>>...>>>...>>...<<<<<....
....>>>>>))<<<<<<<<<...<<...<<<<....<<<<..<<<..<<<
<<.<<<<.<<...[[....>>>>>><<<<<<<.<<..<<<<<<<<<<...
<<.....>>.>>>>>>....>>>>..>>>>>>>>>...<<<<<....>>>
>>....>>>>>...>>>...>>>>.........>>>>...>>......>>
>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<<....>>>>>
..>>>>..<<<<....<<<<<<<<.<<.........<.....<<<<<...
.<<..>>.....>>>>><<<<<<<<...>>>>>>>>............<<
<<<<<<<..<<...........>>..<<<<<<<.....<<<.....>>>.
......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<...<<...<<<<<
....>>>>>...>>...>>>>>>>>..>>>>>..........<.......
....<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..
>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>
..<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<.<<<.<<<<
<.<<<<<<<<<<<<<<<>>>>>>>>>>>>>>>...<<<<<<.......>.
>>>>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<....
.......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.
....<....(((.>....>>>>>>>...<<<<..<<<......>>>..>>
>>.<<<....>>>.>>))).......<<<<<<<.<<..))))..>>>>>>
>>>.............>>>>..>>>>>>.......<<<<...<<<<<<..
.<<.......>>...>>>>>>....>>>.>.......<.<<<.<<<<<<.
.<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>
>>>..>...>>>..>....<<<<<<<....>>>.>>>>........>>>>
>>>>>>>....<<<<<<<...<<<<..<<<<<<<...............>
>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.]].>>>>>>...>>>>
>...>>>>.>>>>>....<<....<<<<<...........>>>>>....>
>..>>>>.......>>>>>>>..<<<.....>>>....<<<<<<<<<...
....>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>.
.>>>>>.>>>...>>>>....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7a0r Ribosome-binding and anti-microbial studies of the mycinamicins, 16-membered macrolide antibiotics from Micromonospora griseorubida.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): K8 M11 T12 P22 Q35 D42 Y44 Q48 R57 N60 K61 P62 M63 G65 H66 K69 R79 E80 R82 K109 G110 K111 G112 T113 Q114 G115 R119 F122 A123 S128 H129 G130 S131 K132 W134 H135 R136 R137 G139 S140 I141 G142 Q143 R144 K145 T146 P147 G148 R149 V150 Y151 K152 G153 K154 R155 M156 A157 H159 R164 T166 Q168 N169 A187 I188 P189 G190 A191 N192 K203
• Binding residue (residue number reindexed from 1): K8 M11 T12 P22 Q35 D42 Y44 Q48 R57 N60 K61 P62 M63 G65 H66 K69 R79 E80 R82 K109 G110 K111 G112 T113 Q114 G115 R119 F122 A123 S128 H129 G130 S131 K132 W134 H135 R136 R137 G139 S140 I141 G142 Q143 R144 K145 T146 P147 G148 R149 V150 Y151 K152 G153 K154 R155 M156 A157 H159 R164 T166 Q168 N169 A187 I188 P189 G190 A191 N192 K203

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7a0r, PDBe:7a0r, PDBj:7a0r
• PDBsum: 7a0r
• PubMed: 34417608
• UniProt: Q9RXK2|RL3_DEIRA Large ribosomal subunit protein uL3 (Gene Name=rplC)