Structure of PDB 6npw Chain B Binding Site BS01

Receptor Information

>6npw Chain B (length=192) Species: 7227 (Drosophila melanogaster)

PSKLAVAVVDSSNMNRSMEAHNFLAKKGFNVRSYGTGERVKLPGMAFDKP
NVYEFGTKYEDIYRDLESKDKEFYTQNGLLHMLDRNRRIKKCPERFQDTK
EQFDIIVTVEERVYDLVVMHMESMESVDNRPVHVLNVDVVNNAEDALMGA
FVITDMINMMAKSTDLDNDIDELIQEFEERRKRVILHSVLFY

Ligand information

• Ligand ID: PO4
• InChI: InChI=1S/H3O4P/c1-5(2,3)4/h(H3,1,2,3,4)/p-3
• InChIKey: NBIIXXVUZAFLBC-UHFFFAOYSA-K
• SMILES:
  CACTVS 3.341: [O-][P]([O-])([O-])=O
  ACDLabs 10.04: [O-]P([O-])([O-])=O
  OpenEye OEToolkits 1.5.0: [O-]P(=O)([O-])[O-]
• Formula: O4 P
• Name: PHOSPHATE ION
• DrugBank: DB14523
• PDB chain: 6npw Chain B Residue 201

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6npw Structural determinants for accurate dephosphorylation of RNA polymerase II by its cognate C-terminal domain (CTD) phosphatase during eukaryotic transcription.
• Resolution: 2.486 Å
• Binding residue (original residue number in PDB): D13 S14 S15 N18 R19 N144
• Binding residue (residue number reindexed from 1): D10 S11 S12 N15 R16 N141
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 3.1.3.16: protein-serine/threonine phosphatase.

Gene Ontology

Molecular Function

• GO:0004721 phosphoprotein phosphatase activity
• GO:0004722 protein serine/threonine phosphatase activity
• GO:0008420 RNA polymerase II CTD heptapeptide repeat phosphatase activity
• GO:0016791 phosphatase activity
• GO:0017018 myosin phosphatase activity

Biological Process

• GO:0006357 regulation of transcription by RNA polymerase II
• GO:0006369 termination of RNA polymerase II transcription
• GO:0006397 mRNA processing
• GO:0031124 mRNA 3'-end processing

Cellular Component

• GO:0005634 nucleus
• GO:0005847 mRNA cleavage and polyadenylation specificity factor complex

External links

• PDB: RCSB:6npw, PDBe:6npw, PDBj:6npw
• PDBsum: 6npw
• PubMed: 30971428
• UniProt: Q9VWE4