Structure of PDB 6ifo Chain B Binding Site BS01

Receptor Information
>6ifo Chain B (length=1360) Species: 301447 (Streptococcus pyogenes serotype M1) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SMDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIG
ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFH
RLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKA
DLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEEN
PINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLT
PNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDA
ILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKE
IFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIP
YYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFD
KNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIV
DLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLK
IIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMK
QLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHD
DSLTFKEDIQKAQVSDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMG
RHKPENIVIEMARENQTTQKNSRERMKRIEEGIKELGSQILKEHPVENTQ
LQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNK
VLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAER
GGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKV
ITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLE
SEFVYGDYKVYDVRKMIAKSEQEITAKYFFYSNIMNFFKTEITLANGEIR
KRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK
SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLF
VEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAEN
IIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYET
RIDLSQLGGD
Ligand information
>6ifo Chain D (length=90) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggugcgcuuggcguuuuagagcuagaaauagcaaguuaaaauaaggcuag
uccguuaucaacuugaaaaaguggcaccgagucggugcuu
............<<<<<<..<<<<....>>>>....>>>>>>..<<....
.>>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6ifo Phage AcrIIA2 DNA Mimicry: Structural Basis of the CRISPR and Anti-CRISPR Arms Race.
Resolution3.313 Å
Binding residue
(original residue number in PDB)		S0 S29 K30 K33 L35 I43 K44 N46 G56 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 K76 N77 R78 Y81 L101 S104 F105 L106 K111 R115 H116 I122 H129 T134 I135 H160 K163 F164 R165 G166 Y325 H328 H329 L332 K336 R340 F351 F352 Y359 I363 Q402 R403 T404 N407 G408 F446 R447 I448 Y450 G453 L455 A456 R457 N459 S460 F462 W464 T472 I473 P475 M495 N497 K500 R661 M694 A728 P731 K735 L738 K742 R753 Q926 Y981 K1085 M1089 P1090 Q1091 K1097 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 W1126 Y1131 M1169 E1225 L1226 A1227 Q1272 H1349 Q1350 Y1356 E1357
Binding residue
(residue number reindexed from 1)		S1 S30 K31 K34 L36 I44 K45 N47 G57 E58 T59 A60 T63 R64 K66 R67 R70 R71 R72 Y73 R75 K77 N78 R79 Y82 L102 S105 F106 L107 K112 R116 H117 I123 H130 T135 I136 H161 K164 F165 R166 G167 Y326 H329 H330 L333 K337 R341 F352 F353 Y360 I364 Q403 R404 T405 N408 G409 F447 R448 I449 Y451 G454 L456 A457 R458 N460 S461 F463 W465 T473 I474 P476 M496 N498 K501 R662 M695 A726 P729 K733 L736 K740 R751 Q921 Y976 K1077 M1081 P1082 Q1083 K1089 E1091 V1092 T1094 G1095 G1096 F1097 I1102 P1104 K1105 R1114 K1115 K1116 W1118 Y1123 M1161 E1217 L1218 A1219 Q1264 H1341 Q1342 Y1348 E1349
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6ifo, PDBe:6ifo, PDBj:6ifo
PDBsum6ifo
PubMed30606466
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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