Structure of PDB 5wws Chain B Binding Site BS01

Receptor Information
>5wws Chain B (length=457) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SIFPKISLRPEVENYLKEGFMNKEIVTALGKQEAERKFETLLKHLSHPPS
FTTVRVNTHLASVQHVKNLLLDELQKQFNGLSVPILQHPDLQDVLLIPVI
GPRKNIKKQQCEAIVGAQCGNAVLRGAHVYAPGIVSASQFMKAGDVISVY
SDIKGKCKKGAKEFDGTKVFLGNGISELSRKEIFSGLPELKGMGIRMTEP
VYLSPSFDSVLPRYLFLQNLPSALVSHVLNPQPGEKILDLCAAPGGKTTH
IAALMHDQGEVIALDKIFNKVEKIKQNALLLGLNSIRAFCFDGTKAVKGE
PPFLPESFDRILLDAPCSGMGQRPNMACTWSVKEVASYQPLQRKLFTAAV
QLLKPEGVLVYSTCTITLAENEEQVAWALTKFPCLQLQPQEPQIGGEGMR
GAGLSCEQLKQLQRFDPSAVPLPDTDMDSLREARREDMLRLANKDSIGFF
IAKFVKC
Ligand information
>5wws Chain D (length=71) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aggguauagcucagggguagagcauuugacagaucaagaggucccugguu
caaauccaggugcccucucca
.<<<<<..<<<<.......>>>>.<<<<<....>>>>>.....<<<<<..
.....>>>>>>>>>>......
Receptor-Ligand Complex Structure
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PDB5wws Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6
Resolution3.247 Å
Binding residue
(original residue number in PDB)		T53 T54 C120 A123 R126 G127 A128 Y131 P133 G134 Q140 F141 K159 K160 G161 R181 F185 G187 L188 K192 G193 P206 S207 F208 D209 L218 Q219 N220 S223 P245 K248 I268 D323 Q331 E343 S346 Y347 P349 L350 R352 K353 C373 F458
Binding residue
(residue number reindexed from 1)		T52 T53 C119 A122 R125 G126 A127 Y130 P132 G133 Q139 F140 K158 K159 G160 R180 F184 G186 L187 K191 G192 P205 S206 F207 D208 L217 Q218 N219 S222 P244 K247 I267 D314 Q322 E334 S337 Y338 P340 L341 R343 K344 C364 F449
Enzymatic activity
Enzyme Commision number 2.1.1.-
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003723 RNA binding
GO:0008168 methyltransferase activity
GO:0008173 RNA methyltransferase activity
GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
GO:0016428 tRNA (cytidine-5-)-methyltransferase activity
Biological Process
GO:0001510 RNA methylation
GO:0002946 tRNA C5-cytosine methylation
GO:0006400 tRNA modification
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5wws, PDBe:5wws, PDBj:5wws
PDBsum5wws
PubMed28531330
UniProtQ8TEA1|NSUN6_HUMAN tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 (Gene Name=NSUN6)

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