Structure of PDB 5klr Chain B Binding Site BS01

Receptor Information

>5klr Chain B (length=426) Species: 10090 (Mus musculus)

GTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLI
PKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLL
ASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLSTL
ACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADS
CWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIV
TGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQ
QVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCGI
IEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLD
KIEALQRHENESVYKASLNLIEKYFS

Ligand information

>5klr Chain A (length=8) Species: 32630 (synthetic construct)

FPAKKRKV

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5klr Contribution of the residue at position 4 within classical nuclear localization signals to modulating interaction with importins and nuclear targeting.
• Resolution: 2.2 Å
• Binding residue (original residue number in PDB): L104 S105 R106 P110 W142 N146 S149 G150 T155 Q181 W184 N188 G191 D192 W231 R238 W273
• Binding residue (residue number reindexed from 1): L33 S34 R35 P39 W71 N75 S78 G79 T84 Q110 W113 N117 G120 D121 W160 R167 W202

Enzymatic activity

• Catalytic site (original residue number in PDB): T151 A163 N361 R366 Q367
• Catalytic site (residue number reindexed from 1): T80 A92 N290 R295 Q296

Gene Ontology

Molecular Function

• GO:0061608 nuclear import signal receptor activity

Biological Process

• GO:0006606 protein import into nucleus

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5klr, PDBe:5klr, PDBj:5klr
• PDBsum: 5klr
• PubMed: 29750988
• UniProt: P52293|IMA1_MOUSE Importin subunit alpha-1 (Gene Name=Kpna2)