Structure of PDB 5hr6 Chain B Binding Site BS01

Receptor Information
>5hr6 Chain B (length=354) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
INLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVL
RGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATL
AVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQ
RPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVP
ALDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEK
SNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGA
PYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTK
RTLR
Ligand information
>5hr6 Chain C (length=68) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ccccuucgucuagaggcccaggacaccgcccuuucacggcgguaacaggg
guucgaauccccuagggg
<<<<<..<<<<.........>>>>.<<<<<.......>>>>>....<<<<
<.......>>>>>>>>>>
Receptor-Ligand Complex Structure
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PDB5hr6 Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA.
Resolution2.88 Å
Binding residue
(original residue number in PDB)		F139 N142 L183 N184 L185 N186 K221 T366 K367 R371
Binding residue
(residue number reindexed from 1)		F122 N125 L166 N167 L168 N169 K204 T349 K350 R354
Enzymatic activity
Enzyme Commision number 2.1.1.192: 23S rRNA (adenine(2503)-C(2))-methyltransferase.
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0002935 tRNA (adenine(37)-C2)-methyltransferase activity
GO:0003824 catalytic activity
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008173 RNA methyltransferase activity
GO:0019843 rRNA binding
GO:0046872 metal ion binding
GO:0051536 iron-sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding
GO:0070040 rRNA (adenine(2503)-C2-)-methyltransferase activity
Biological Process
GO:0006364 rRNA processing
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
GO:0046677 response to antibiotic
GO:0070475 rRNA base methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Biological Process
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Cellular Component
External links
PDB RCSB:5hr6, PDBe:5hr6, PDBj:5hr6
PDBsum5hr6
PubMed27081063
UniProtP36979|RLMN_ECOLI Dual-specificity RNA methyltransferase RlmN (Gene Name=rlmN)

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