Structure of PDB 5bir Chain B Binding Site BS01

Receptor Information

>5bir Chain B (length=104) Species: 5062 (Aspergillus oryzae)

ACDYTCGSNCYSSSDVSTAQAAGYKLHEDGETVGSNSYPHKYNNYEGFDF
SVSSPYYEWPILSSGDVYSGGSPGADRVVFNENNQLAGVITQTGASGNNF
VECT

Ligand information

• Ligand ID: CA
• InChI: InChI=1S/Ca/q+2
• InChIKey: BHPQYMZQTOCNFJ-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Ca++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Ca+2]
• Formula: Ca
• Name: CALCIUM ION
• DrugBank: DB14577
• PDB chain: 5bir Chain B Residue 601

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5bir Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position.
• Resolution: 2.0 Å
• Binding residue (original residue number in PDB): G47 D49
• Binding residue (residue number reindexed from 1): G47 D49
• Annotation score: 4

Enzymatic activity

• Catalytic site (original residue number in PDB): Y38 H40 E58 D76 R77 T91 Q92 F100
• Catalytic site (residue number reindexed from 1): Y38 H40 E58 D76 R77 T91 Q92 F100
• Enzyme Commision number: 4.6.1.24: ribonuclease T1.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004540 RNA nuclease activity
• GO:0016829 lyase activity
• GO:0046589 ribonuclease T1 activity

Biological Process

• GO:0008150 biological_process

Cellular Component

• GO:0001411 hyphal tip
• GO:0030428 cell septum

External links

• PDB: RCSB:5bir, PDBe:5bir, PDBj:5bir
• PDBsum: 5bir
• PubMed: 9466938
• UniProt: P00651|RNT1_ASPOR Guanyl-specific ribonuclease T1 (Gene Name=rntA)