Structure of PDB 4ne9 Chain B Binding Site BS01

Receptor Information

>4ne9 Chain B (length=490) Species: 9606 (Homo sapiens)

SIPWNLERITPPRYRAGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVP
EEDQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTL
IGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAA
GNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPG
EDIIGASSDCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRL
IHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHQLFCRTVWSAHSGPTR
MATAIARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEG
VYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVE
DLGPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWT
LTGCSALPGTSHVLGAYAVDNTCVVRSRAVTAVAICCRSR

Ligand information

>4ne9 Chain D (length=26) Species: 9606 (Homo sapiens)

GTNECLDNNGGCSHVCNDLKIGYECL

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4ne9 Design and synthesis of truncated EGF-A peptides that restore LDL-R recycling in the presence of PCSK9 in vitro.
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): R194 I369 C375 T377 C378 F379 V380
• Binding residue (residue number reindexed from 1): R35 I204 C210 T212 C213 F214 V215

Enzymatic activity

• Catalytic site (original residue number in PDB): D186 H226 N317 S386
• Catalytic site (residue number reindexed from 1): D27 H61 N152 S221
• Enzyme Commision number: 3.4.21.-

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:4ne9, PDBe:4ne9, PDBj:4ne9
• PDBsum: 4ne9
• PubMed: 24440079
• UniProt: Q8NBP7|PCSK9_HUMAN Proprotein convertase subtilisin/kexin type 9 (Gene Name=PCSK9)