Structure of PDB 4ncb Chain B Binding Site BS01

Receptor Information
>4ncb Chain B (length=672) Species: 274 (Thermus thermophilus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
GKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGREEVYPLLAQVARRAG
GVTVRMGDGLASWSPPEVLVLEGTLARMGQTYAYRLYPKGRRPLDPKDPG
ERSVLSALARRLLQERLRRLEGVWVEGLAVYRREHARGPGWRVLGGAVLD
LWVSDSGAFLLEVDPAYRILCEMSLEAWLAQGHPLPKRVRNAYDRRTWEL
LRLGEEDPKELPLPGGLSLLDYHASKGRLQGREGGRVAWVADPIPHLTGL
LVPVLTLEDLHESLALSLPWEERRRRTREIASWIGRRLGLGTPEAVRAQA
YRLSIPKLMGRRAVSKPADALRVGFYRAQETALALLRLDGAQGWPEFLRR
ALLRAFGASGASLRLHTLHAHPSQGLAFREALRKAKEEGVQAVLVLTPPM
AWEDRNRLKALLLREGLPSQILNVPLREEERHRWENALLGLLAKAGLQVV
ALSGAYPAELAVGFDAGGRESFRFGGAACAVGGDGGHLLWTLPEAQAGER
IPQEVVWDLLEETLWAFRRKAGRLPSRVLLLRDGRVPQDEFALALEALAR
EGIAYDLVSVRKSGGGRVYPVQGRLADGLYVPLEDKTFLLLTVHRDFRGT
PRPLKLVHEAGDTPLEALAHQIFHLTRLYPASGFAFPRLPAPLHLADRLV
KEVGRLGIRHLKEVDREKLFFV
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB4ncb Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.
Resolution2.189 Å
Binding residue
(original residue number in PDB)
Y43 R59 A170 Y171 R172 I173 R192 T201 L265 L267 L279 S280 L281 R286 P412 M413 W415 K422 Q433 I434 N436 R446 N449 K457 E512 R513 R580 R611 T613 P614 R615 Y642 A644 S645 P650 R651 H657 R661 V685
Binding residue
(residue number reindexed from 1)
Y39 R55 A166 Y167 R168 I169 R188 T197 L255 L257 L266 S267 L268 R273 P399 M400 W402 K409 Q420 I421 N423 R433 N436 K444 E499 R500 R567 R598 T600 P601 R602 Y629 A631 S632 P637 R638 H644 R648 V672
Enzymatic activity
Enzyme Commision number 3.1.24.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006260 DNA replication
GO:0044355 clearance of foreign intracellular DNA

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Molecular Function

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Biological Process
External links
PDB RCSB:4ncb, PDBe:4ncb, PDBj:4ncb
PDBsum4ncb
PubMed24374628
UniProtQ746M7|AGO_THET2 Protein argonaute (Gene Name=ago)

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