Structure of PDB 4hcc Chain B Binding Site BS01

Receptor Information

>4hcc Chain B (length=463) Species: 668369 (Escherichia coli DH5[alpha])

QSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDY
LPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRF
DDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPEN
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFT
HRNKHKLMALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVI
MVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLA
QANTLRPEDADRLGINRQHCLDNLKILRENPQVREKVVAIFAEFTPSDNV
DAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLLFNYRAR
NFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVAL
LKALWQYADEIVH

Ligand information

>4hcc Chain D (length=10)

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 4hcc The structures of Escherichia coli exonuclease I in complex with the single strand DNA
• Resolution: 2.96 Å
• Binding residue (original residue number in PDB): N103 F107 R113 Y124 W128 L138 R142 F164 R165 K214 G234 M235 G237 A238 N255 A286 K287 N304 F357 Y368 F371
• Binding residue (residue number reindexed from 1): N96 F100 R106 Y117 W121 L131 R135 F154 R155 K204 G224 M225 G227 A228 N245 A276 K277 N294 F344 Y355 F358

Enzymatic activity

• Enzyme Commision number: 3.1.11.1: exodeoxyribonuclease I.

Gene Ontology

Molecular Function

• GO:0000175 3'-5'-RNA exonuclease activity
• GO:0000287 magnesium ion binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003697 single-stranded DNA binding
• GO:0004527 exonuclease activity
• GO:0004529 DNA exonuclease activity
• GO:0005515 protein binding
• GO:0008310 single-stranded DNA 3'-5' DNA exonuclease activity
• GO:0046872 metal ion binding
• GO:0051575 5'-deoxyribose-5-phosphate lyase activity

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006274 DNA replication termination
• GO:0006281 DNA repair
• GO:0006308 DNA catabolic process

External links

• PDB: RCSB:4hcc, PDBe:4hcc, PDBj:4hcc
• PDBsum: 4hcc
• UniProt: P04995|EX1_ECOLI Exodeoxyribonuclease I (Gene Name=sbcB)