Structure of PDB 4fbu Chain B Binding Site BS01

Receptor Information

>4fbu Chain B (length=341) Species: 273057 (Saccharolobus solfataricus P2)

MIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEA
RKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIE
IASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVF
AKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGIN
KLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIVT
MKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGR
TFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFI

Ligand information

>4fbu Chain D (length=13)

cccaataccagtc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4fbu Structural mechanism of replication stalling on a bulky amino-polycyclic aromatic hydrocarbon DNA adduct by a y family DNA polymerase.
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): Y12 A57 D105 P184 G185 G187 I189 T190 V296 S297 R298 T301 K339
• Binding residue (residue number reindexed from 1): Y12 A57 D105 P184 G185 G187 I189 T190 V296 S297 R298 T301 K339

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0042276 error-prone translesion synthesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4fbu, PDBe:4fbu, PDBj:4fbu
• PDBsum: 4fbu
• PubMed: 23876706
• UniProt: Q97W02|DPO4_SACS2 DNA polymerase IV (Gene Name=dbh)