Structure of PDB 4ep4 Chain B Binding Site BS01

Receptor Information

>4ep4 Chain B (length=166) Species: 300852 (Thermus thermophilus HB8)

MVVAGIDPGITHLGLGVVAVEGKGALKARLLHGEVVKTSPQEPAKERVGR
IHARVLEVLHRFRPEAVAVEEQFFYRQNELAYKVGWALGAVLVAAFEAGV
PVYAYGPMQVKQALAGHGHAAKEEVALMVRGILGLKEAPRPSHLADALAI
ALTHAFYARMGTAKPL

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 4ep4 Chain B Residue 201

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4ep4 Structural asymmetry in the Thermus thermophilus RuvC dimer suggests a basis for sequential strand cleavages during Holliday junction resolution.
• Resolution: 1.28 Å
• Binding residue (original residue number in PDB): D7 H143
• Binding residue (residue number reindexed from 1): D7 H143
• Annotation score: 4

Enzymatic activity

• Enzyme Commision number: 3.1.21.10: crossover junction endodeoxyribonuclease.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0008821 crossover junction DNA endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006281 DNA repair
• GO:0006310 DNA recombination

Cellular Component

• GO:0005737 cytoplasm
• GO:0048476 Holliday junction resolvase complex

External links

• PDB: RCSB:4ep4, PDBe:4ep4, PDBj:4ep4
• PDBsum: 4ep4
• PubMed: 23118486
• UniProt: Q5SJC4|RUVC_THET8 Crossover junction endodeoxyribonuclease RuvC (Gene Name=ruvC)