Structure of PDB 3u7f Chain B Binding Site BS01

Receptor Information
>3u7f Chain B (length=379) Species: 10090 (Mus musculus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SSLGWESLKKLLVFTASGVKPQGKVAAFALDGTLITTRSGKVFPTSPSDW
RILYPEIPKKLQELAAEGYKLVIFTNQMGIGRGKLPAEVFKGKVEAVLEK
LGVPFQVLVATHAGLNRKPVSGMWDHLQEQANEGIPISVEDSVFVGDAAG
RLANWAPGRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFD
PRTISSAGPLYLPESSSLLSPNPEVVVAVGFPGAGKSTFIQEHLVSAGYV
HVNRDTLGSWQRCVSSCQAALRQGKRVVIDNTNPDVPSRARYIQCAKDAG
VPCRCFNFCATIEQARHNNRFREMTDPSHAPVSDMVMFSYRKQFEPPTLA
EGFLEILEIPFRLQLDPALQRLYRQFSEG
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB3u7f Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates.
Resolution1.8 Å
Binding residue
(original residue number in PDB)		D172 N217 Q218 G220 R258 F305
Binding residue
(residue number reindexed from 1)		D31 N76 Q77 G79 R117 F164
Binding affinityPDBbind-CN: Kd=600nM
Enzymatic activity
Enzyme Commision number 2.7.1.78: polynucleotide 5'-hydroxyl-kinase.
3.1.3.32: polynucleotide 3'-phosphatase.
Gene Ontology
Molecular Function
GO:0046403 polynucleotide 3'-phosphatase activity
GO:0046404 ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
Biological Process
GO:0006281 DNA repair

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Molecular Function
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Biological Process
External links
PDB RCSB:3u7f, PDBe:3u7f, PDBj:3u7f
PDBsum3u7f
PubMed22171004
UniProtQ9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Gene Name=Pnkp)

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