Structure of PDB 3u6b Chain B Binding Site BS01

Receptor Information

>3u6b Chain B (length=376) Species: 83333 (Escherichia coli K-12)

KEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAAKARGITIN
TSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMP
QTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFP
GDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLP
IEDVFSITVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDE
GRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGG
RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIA
MDDGLRFAIREGGRTVGAGVVAKVLG

Ligand information

>3u6b Chain C (length=12) Species: 35762 (Planobispora rosea)

SCNCVCGFCCSC

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3u6b Antibacterial optimization of 4-aminothiazolyl analogues of the natural product GE2270 A: identification of the cycloalkylcarboxylic acids.
• Resolution: 2.12 Å
• Binding residue (original residue number in PDB): E215 D216 F218 V226 T228 T256 E259 F261 R262 N273 V274 G275 L277
• Binding residue (residue number reindexed from 1): E202 D203 F205 V209 T211 T239 E242 F244 R245 N256 V257 G258 L260

Enzymatic activity

• Catalytic site (original residue number in PDB): D21 K24 T25 T61 H84
• Catalytic site (residue number reindexed from 1): D20 K23 T24 T48 H71

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0032045 guanyl-nucleotide exchange factor complex

External links

• PDB: RCSB:3u6b, PDBe:3u6b, PDBj:3u6b
• PDBsum: 3u6b
• PubMed: 21999529
• UniProt: P0CE47|EFTU1_ECOLI Elongation factor Tu 1 (Gene Name=tufA)