Structure of PDB 3tb3 Chain B Binding Site BS01

Receptor Information

>3tb3 Chain B (length=204) Species: 9606 (Homo sapiens)

EWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFEKLKPVHGLIFLF
KWQPGEEPAGSVVQDSRLDTIFFAKQVINNAAATQAIVSVLLNCTHQDVH
LGETLSEFKEFSQSFDAAMKGLALSNSDVIRQVHNSFARAFHFVSYVPVN
GRLYELDGLREGPIDLGACNQDDWISAVRPVIEKRIQKYSEGEIRFNLMA
IVSD

Ligand information

• Ligand ID: CA
• InChI: InChI=1S/Ca/q+2
• InChIKey: BHPQYMZQTOCNFJ-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Ca++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Ca+2]
• Formula: Ca
• Name: CALCIUM ION
• DrugBank: DB14577
• PDB chain: 3tb3 Chain B Residue 229

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3tb3 Length of the active-site crossover loop defines the substrate specificity of ubiquitin C-terminal hydrolases for ubiquitin chains.
• Resolution: 2.3 Å
• Binding residue (original residue number in PDB): E109 E113
• Binding residue (residue number reindexed from 1): E103 E107
• Annotation score: 4

Enzymatic activity

• Catalytic site (original residue number in PDB): Q82 A88 H164 D179
• Catalytic site (residue number reindexed from 1): Q76 A82 H142 D157
• Enzyme Commision number: 3.4.19.12: ubiquitinyl hydrolase 1.

Gene Ontology

Molecular Function

• GO:0004843 cysteine-type deubiquitinase activity
• GO:0070628 proteasome binding

Biological Process

• GO:0006511 ubiquitin-dependent protein catabolic process
• GO:0016579 protein deubiquitination

Cellular Component

• GO:0031011 Ino80 complex

External links

• PDB: RCSB:3tb3, PDBe:3tb3, PDBj:3tb3
• PDBsum: 3tb3
• PubMed: 21851340
• UniProt: Q9Y5K5|UCHL5_HUMAN Ubiquitin carboxyl-terminal hydrolase isozyme L5 (Gene Name=UCHL5)