Structure of PDB 3cw2 Chain B Binding Site BS01

Receptor Information

>3cw2 Chain B (length=400) Species: 2287 (Saccharolobus solfataricus)

AWPKVQPEVNIGVVGHVDHGKTTLVQAITGIWTSTIKLGYAETNIGVCES
CKKPEAYVTEPSCKSCGSDDEPKFLRRISFIDAPGHEVLMATMLSGAALM
DGAILVVAANEPFPQPQTREHFVALGIIGVKNLIIVQNKVDVVSKEEALS
QYRQIKQFTKGTWAENVPIIPVSALHKINIDSLIEGIEEYIKTPYRDLSQ
KPVMLVIRSFDVNKPGTQFNELKGGVIGGSIIQGLFKVDQEIKVLPGLRV
EKQGKVSYEPIFTKISSIRFGDEEFKEAKPGGLVAIGTYLDPSLTKADNL
LGSIITLADAEVPVLWNIRIKYNLLGAKEMLKVDPIRAKETLMLSVGSST
TLGIVTSVKKDEIEVELRRPVAVWSNNIRTVISRQIAGRWRMIGWGLVEI

Ligand information

>3cw2 Chain L (length=21) Species: 2287 (Saccharolobus solfataricus)

SSEKEYVEMLDRLYSKLPEKG

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3cw2 Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits.
• Resolution: 2.8 Å
• Binding residue (original residue number in PDB): E66 V151 Y163 K167 P182 V183 I189 N190 D192
• Binding residue (residue number reindexed from 1): E55 V140 Y152 K156 P171 V172 I178 N179 D181

Enzymatic activity

• Catalytic site (original residue number in PDB): D19 K22 T23 T46 H97
• Catalytic site (residue number reindexed from 1): D18 K21 T22 T35 H86
• Enzyme Commision number: 3.6.5.3: protein-synthesizing GTPase.

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003743 translation initiation factor activity
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001731 formation of translation preinitiation complex
• GO:0006412 translation
• GO:0006413 translational initiation
• GO:0006414 translational elongation

External links

• PDB: RCSB:3cw2, PDBe:3cw2, PDBj:3cw2
• PDBsum: 3cw2
• PubMed: 18675278
• UniProt: Q980A5|IF2G_SACS2 Translation initiation factor 2 subunit gamma (Gene Name=eif2g)