Structure of PDB 2gij Chain B Binding Site BS01

Receptor Information
>2gij Chain B (length=236) Species: 727 (Haemophilus influenzae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SFIKPIYQDINSILIGQFEKLVYKFLKENLSDLTFKQYEYLNDLFMKNPA
IIGHEARYKLFNSPTLLFLLSRGKAATENWSIENLFEEKQNDTADILLVK
DQFYELLDVKTRNISKSAFAPNIISAYKLAQTCAKMIDNKEFDLFDINYL
EVDWEGEDLVCVSTSFAELFKSEPSELYINWAAAMQIQFHVRDLDQGFNG
TREEWAKSYLKHFVTQAEQRAISMIDKFVKPFKKYI
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB2gij Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism.
Resolution1.93 Å
Binding residue
(original residue number in PDB)		Q109 N110 S136 A137 F138 P140 N141 S144 K147 A205 Q207
Binding residue
(residue number reindexed from 1)		Q90 N91 S117 A118 F119 P121 N122 S125 K128 A184 Q186
Binding affinityPDBbind-CN: Kd=0.42nM
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:2gij, PDBe:2gij, PDBj:2gij
PDBsum2gij
PubMed16675462
UniProtP17743|T2C2_HAEIF Type II restriction enzyme HincII (Gene Name=hincIIR)

[Back to BioLiP]