Structure of PDB 2gih Chain B Binding Site BS01

Receptor Information
>2gih Chain B (length=251) Species: 727 (Haemophilus influenzae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SFIKPIYQDINSILIGQKVKRSGHAAGEPFEKLVYKFLKENLSDLTFKQY
EYLNDLFMKNPAIIGHEARYKLFNSPTLLFLLSRGKAATENWSIENLFEE
KQNDTADILLVKDQFYELLDVKTRNISKSAFAPNIISAYKLAQTCAKMID
NKEFDLFDINYLEVDWELNGEDLVCVSTSFAELFKSEPSELYINWAAAMQ
IQFHVRDLDQGFNGTREEWAKSYLKHFVTQAEQRAISMIDKFVKPFKKYI
L
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB2gih Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism.
Resolution2.5 Å
Binding residue
(original residue number in PDB)		Q109 N110 D127 T130 K135 A137 F138 A139 P140 N141 S144 K147 A205 M206 Q207
Binding residue
(residue number reindexed from 1)		Q102 N103 D120 T123 K128 A130 F131 A132 P133 N134 S137 K140 A198 M199 Q200
Binding affinityPDBbind-CN: Kd=1.03nM
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:2gih, PDBe:2gih, PDBj:2gih
PDBsum2gih
PubMed16675462
UniProtP17743|T2C2_HAEIF Type II restriction enzyme HincII (Gene Name=hincIIR)

[Back to BioLiP]