Structure of PDB 1t9j Chain B Binding Site BS01

Receptor Information
>1t9j Chain B (length=151) Species: 3055 (Chlamydomonas reinhardtii) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
TKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSLTFQVTEKTQRR
WFLDKLVDEIGVGYVRDRGSVSDYILSEIKPLHNFLTQLQPFLKLKQKQA
NLVLKIIEQLPSAKESPDKFLEVCTWVDQIAALNDSKTRKTTSETVRAVL
D
Ligand information
Receptor-Ligand Complex Structure
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PDB1t9j Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease.
Resolution2.0 Å
Binding residue
(original residue number in PDB)
S332 Y333 K334 Q338 R368 R370 I381 K416 D437 K439
Binding residue
(residue number reindexed from 1)
S30 Y31 K32 Q36 R66 R68 I79 K114 D135 K137
Binding affinityPDBbind-CN: Kd=0.6nM
Enzymatic activity
Catalytic site (original residue number in PDB) G319 D320
Catalytic site (residue number reindexed from 1) G17 D18
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0006314 intron homing
Cellular Component
GO:0009507 chloroplast

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Cellular Component
External links
PDB RCSB:1t9j, PDBe:1t9j, PDBj:1t9j
PDBsum1t9j
PubMed15518550
UniProtP05725|DNE1_CHLRE DNA endonuclease I-CreI

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