Structure of PDB 6uz7 Chain AY Binding Site BS01

Receptor Information

>6uz7 Chain AY (length=125) Species: 28985 (Kluyveromyces lactis)

KQSLDVSSDRRKARKAYFTAPSSERRVLLSAPLSKELREQYNIKALPIRK
EDEVLVVRGSKKGQEGKVSSVYRLKFAVQVDKLTKEKSNGASVPTNIHPS
KVVITKLHLDKDRKALIQRKGGKLE

Ligand information

>6uz7 Chain 5 (length=3269)

uugaccucaaaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucuggcgucuucgacguccgaguuguaau
uugaagaaggcaacuuuguagcugguccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggaucccaguuauuugu
aaagugcuuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugggccagcauc
aguuuuagcgguuggauaaauccucgggaauguggcucugcuucgguaga
guguuauagcccgugggaauacagccagcugggacugaggauugcgacuu
uugucaaggaugcuggcguaaugguuaaaugccgcccgucuugaaacacg
gaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccguacg
cguaaugaaagugaacguaggugagggcccgcaagggugcaucaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
ugauuagagguaccgggguugaaaugaccuugaccuauucucaaacuuua
aauauguaagaaguccuuguugcuuaauugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguggaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacuccaccgucaggguuaauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuaggcu
guaaagcuggguagaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uucaaaggccuaauuuucuaggccaccaucgaaagggaauccgguuaaua
uuccggaaccuggauauggauucuucacgguaacguaacugaauguggag
acgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuau
caccccggaauugguuuauccggagagggggucuuauggcuggaagagcc
cagcccuugugcuggguccggugcgcccgcgacggcccuugaaaauccac
aggaaggaauaguuuucaugccaggucguacugauaaccgcagcaggucu
ccaaggugaacagccucuaguugauagaauaauguagauaagggaagucg
gcaaaauagauccguaacuucgggauaaggauuggcucuaaggaucggaa
gugagggccuuggucagacgcggcguuguagacggccuugguaggucucu
uguagaucugcaauuaacgaucaacuuagaacugguacggacaaggggaa
ucugacugucuaauuaaaacauagcauugcgauggucagaaagugauguu
gacgcaaugugauuucugcccagugcucugaaugucaaagugaagaaauu
caaccaagcgcggguaaacggcgggaguaacuaugacucucuuaagguag
ccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaacgaga
uucccacugucccuaucuacuaucuagcgaaaccacagccaagggaacgg
gcuuggcagaaucagcggggaaagaagacccuguugagcuugacucuagu
uugacauugugaagagacauagaggguguagcauaagugggagcuucggc
gccagugaaauaccacuaccuuuauaguuucuuuacuuauucaauuaagc
ggagcuggaauucauuuuccacguucuagcauucaaaguccuauacgggc
ugauccggguugaagacauugucagguggggaguuuggcuggggcggcac
aucuguuaaacgauaacgcagauguccuaagggggacucauggagaacag
aaaucuccaguagaacaaaaggguaaaaguccccuugauuuugauuuuca
gugugaauacaaaccaugaaaguguggccuaucgauccuuuagguccucg
gaauuugaggcuagaggugccagaaaaguuaccacagggauaacuggcuu
guggcagucaagcguucauagcgacauugcuuuuugauucuucgaugucg
gcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguucac
ccacuaauagggaacgugagcuggguuuagaccgucgugagacagguuag
uuuuacccuacugaugaauguuaucgcaauaguaauugaacuuaguacga
gaggaacaguucauucggauaauugguuuuugcggcugucugaucaggca
uugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaaguca
gaauccaugcuagaacgcgaugauuucuuugccuugcacaauauagaagg
auaagaauaaggcgucuuuuuggcgucgcugaaccauagcaggcuggcaa
cggugcucuuagcggaaaggcuuugggugcuugccggcgaauugcaaugu
cauuuugcgcaaggauaaaucauuuguauacgacuuaaauguacaacagg
guauuguaagcaguagaguagccuuguuguuacgaucugcugagauuaag
ccuucguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<........<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<.<<<<......<<<<<<<....>>>>>
>>.......>>>>.>>.>.>.>>>>>.>>>>>>>>>>........<<<..
..>>>.....>>>>>>>.....>>>>>>>>>>>..<<<<........>>>
><<<<<<.....<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>
>...<<....>>.......<<<<<..<<<<....>>>>...>>>>>.<<<
<<<<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>...<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<<<<<<<<<....<<.<<<<<<<.....<<<....
<<<....<<<<..<<<<<<<.....>>>>>>>>>>>....>>>..>.>>.
....>>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>..
>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<.....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
......<<<<<<......>>>>>>....>>....>>...<<<<<......
..>>>>>))<<<<.<<<<<<<...<.<<<....>>>.......<<<<<<.
..<<<<.<<<<.<<.<<<<<<<<<<..{{{..>>>>>>....<<<<<...
.<<<<<.....<<<.....>>>.....>>>>>.....>>>>>.....<<<
<<<<......>>>>>>>>>>>...>>.>>>>.>>>>.........>>>>>
>.....>....>>>..>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.......
..<.....<<<<<...............>>>>><<.<<<<<<<<<<<...
..<...<<<..<<<<....<<<<..>>>>....>>>>..>>>........
.......>.........>>>>>..>>>>>>.>>............<<<<<
<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>...
...>>>>>>>...<<..<<<..<<<<<<....<<<....<<<<<....>>
>>.>...>>>.>>>>..>>>>><<<<<<.......>>>>>>........<
...........<<<<<.......>>>>>.....>.....>>....>>.>>
>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<.......
.>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<<
<<<<<<..<<<<<..<.....<<<<..........<<<<<..<<....>>
............>>>>>.>>>>.....>...>>>>>...<<<<<.....<
<<<................................<..<.<.....>>..
>..>>>>..>>>>>.....>>>>>>>>..>>>>>....<<.....>>...
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<...
....>>>>.>>>>.<<<....>>>.>>))).......((((((..((((.
>>>>>>))))))))))..........]]]]..}}]]]].......<<.<.
<.<<<<<<...<<.......>>...>>>>>>...>>.>.>.......<<<
<<..<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........
>>>.....>>>>>......>>>.>>....<<<<<<<....>>>.>>>>..
..}}..}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<........
.......>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>
..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>
...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....<<
..........<<<<<<.....>>>>>>......>>.......<<<<<<..
.<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>............
.>>.>>>.>>>>>>..<<<<<.<.............>.....<<<<<<<<
<.<<<....<<<<....<<<<<......>>>>>...>>>>...>>>....
>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6uz7 Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): Q4 S5 V8 S9 S10 R12 K14 R16 Y19 V29 L30 K46 R60 G61 S62 K87 S90 N91 H100 S102 K103 R121 K122
• Binding residue (residue number reindexed from 1): Q2 S3 V6 S7 S8 R10 K12 R14 Y17 V27 L28 K44 R58 G59 S60 K85 S88 N89 H98 S100 K101 R119 K120

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:0032991 protein-containing complex
• GO:0043232 intracellular non-membrane-bounded organelle

External links

• PDB: RCSB:6uz7, PDBe:6uz7, PDBj:6uz7
• PDBsum: 6uz7
• PubMed: 31900355
• UniProt: Q6CW97