Structure of PDB 6p5i Chain AW Binding Site BS01

Receptor Information

>6p5i Chain AW (length=63) Species: 9986 (Oryctolagus cuniculus)

MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQIN
WTVLYRRKHKKGQ

Ligand information

>6p5i Chain 5 (length=3594)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcggguccggccgugccggcggggcgga
ucucccgcucccgucggcgggggaccgccccccggccggcgaccggccgc
cgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacg
gcugggaaggcccggcggggaagguggcccgggggccccggguguuacag
cccacccggcagcagcagcucgccgaaucccggggccgagggagcgagac
cugugucgccgcgcucuccccccugggggggccgggccaccccucccacg
gcgcgaccgcuggggcggacuguccagugcgccccgggcgggucgcgccg
ucgggcccggggggccacgcgucccccgcgagcgcacggggucggcggcg
augucggcuacccacccgacccgucuugaaacacggaccaaggagucuaa
cacgugcgcgagucgggggcuacgaaagccgccguggcgcaaugaaggug
aaggccggcgccgccggccgaggugggaucccgaggccuuccaguccgcc
gagggcgcaccaccggcccgucucgcccgccgcgccggggagguggagca
cgagcgcacguguuaggacccgaaagauggugaacuaugccugggcaggg
cgaagccagaggaaacucugguggagguccguagcgguccugacgugcga
aucggucguccgaccuggguauaggggcgaaagacuaaucgaaccaucua
guagcugguucccuccaaaguuucccucaggauagcuggcgcucuccgca
guuuuauccgguaaagcgaaugauuagaggucuuggggccgaaacgaucu
caaccuauucucaaacuuuaaauggguaagaagcccggcucgcuggugug
gagccgggcguggaaugcgagugccuagugggccacuuuugguaagcaga
acuggcgcugcgggaugaaccgaacgccggguuaaggcgcccgaugccga
cgcucaucagaccccagaaaagguguugguugauauagacagcaggaugg
uggccauggaagucggaauccgcuaaggaguguguaacaacucaccugcc
gaaucaacuagcccugaaaauggauggcgcuggagcgucgggcccauacc
cggccgucgcuggcagucggcagagcgagaaagagaguggcgcggacgcu
acgccgcgacgaguaggagggccgcugcggugagccuugaagccuagggc
gcgggcccggguggagccgccgcaggugcagaucuuggugguaguagcaa
auauucaaacgagaacuuugaaggccgaaguggagaaggguuccauguga
acagcaguugaacaugggucagucgguccugagagaugggcgagcgccgu
uccgaagggacgggcgauggccuccguugcccucagccgaucgaaaggga
gucggguucagauccccgaauccggaguggcggagaugggcgccgcgagg
cguccagugcgguaacgcgaccgaucccggagaagccggcgggagccccg
gggagaguucucuuuucuuugugaagggcagggcgcccuggaauggguuc
gccccgagagaggggcccgugccuuggaaagcgucgcgguuccggcggcg
uccggugagcucucgcuggcccuugaaaauccgggggagaggguguaaau
cucgcgccgggccguacccauauccgcagcaggucuccaaggugaacagc
cucuggcauguuggaacaauguagguaagggaagucggcaagccggaucc
guaacuucgggauaaggauuggcucuaagggcuggcagccgacuuagaac
uggugcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcga
aggcccgcggcggguguugacgcgaugugauuucugcccagugcucugaa
ugucaaagugaagaaauucaaugaagcgcggguaaacggcgggaguaacu
augacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgc
augaauggaugaacgagauucccacugucccuaccuacuauccagcgaaa
ccacagccaagggaacgggcuuggcggaaucagcggggaaagaagacccu
guugagcuugacucuagucuggcacggugaagagacaugagagguguaga
auaagugggaggcccgggccgccggugaaauaccacuacucugaucguuu
uuucacugacccggugaggcgggggggcgagccgggcucucgcuucuggc
gccaagcgcccggccggccgggcgcgacccgcuccggggacagugccagg
uggggaguuugacuggggcgguacaccugucaaacgguaacgcagguguc
cuaaggcgagcucagggaggacagaaaccucccguggagcagaagggcaa
aagcucgcuugaucuugauuuucaguacgaauacagaccgugaaagcggg
gccucacgauccuucugaccuuuuggguuuuaagcaggaggugucagaaa
aguuaccacagggauaacuggcuuguggcggccaagcguucauagcgacg
ucgcuuuuugauccuucgaugucggcucuuccuaucauugugaagcagaa
uucaccaagcguuggauuguucacccacuaauagggaacgugagcugggu
uuagaccgucgugagacagguuaguuuuacccuacugaugauguguuguu
gccaugguaauccugcucaguacgagaggaaccgcagguucagacauuug
guguaugugcuuggcugaggagccaauggggcgaagcuaccaucuguggg
auuaugacugaacgccucuaagucagaaucccgcccaggcggaacgauag
cgccggagccucgguuggccucggauagccggucccccgccgggguccgg
ugcggagagcccuucgucccgggaaacggggcggccggaaagggggccgc
ccucucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaacc
auucguagacgaccugcuucugggucgggguuucguacguagcagagcag
cugcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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>>>>>>..<<<..<<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>>..<<<<<<<<<....<<<<<<<<..<<<<<<<<....<<<<..
....>>>>.....((......<<<.....))>>>...>.>>>>>>>...>
>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>..
...<<<<<..((((((>>>>>..>>>........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<<.<<...<<
<<<<<..<<<.....<<<<<<<<<....<<<<<<<.<<<<<.<<<<<<<.
<<<<<<<<........>>>>>>>>>>>>>>>>>>>>>>>>...>>>..>>
>>>>>>>......>>>..>>.>>>>>...<<<<<<<<<<<<<<<<<<.<<
<<...<<..<<<.....<<<......<<<<<<<<.>>>>>>>>.......
>>>....>>>.........>>>>>>...>>>>>>>>>>.....<<.....
{.>>......<<<<<..<<<<<<..>>>>>>....<<<...<<.<<<<<<
<<<<<<<<<<<<<<<<<.<<<<..>>>>.>>>>>>>.>>.>>>>>>>>>>
>.>>>...>>.>>.>...............>>>>>.....>>>.>>>>>.
..>>>>>...>>>>>>>>..<<<<........>>>><<<<<<...<<<<<
<<<<<<<<<..<<<...<<<<.....>>>>.....>>>...<<....>>.
..<<<<<<.....>>>>>>..<<<<<...<<<..<<<....[[.[[.>>>
..>>>....>>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>.
...>>>>>>>>>>>>>>.<<.<..<<<<<<<.....<<<<<<<<<..<<<
....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.......
.>>>>.>>>....>>>>>>>>>...<<.......>>......>>>>>>..
.>..>.>>..<<<<<<....<.<<<....>>>.><<.<<<<<<<...<<<
.<<<<<<<<<...<<<....<<<....<<<..<<<<.<.........>.>
>>>>>>....>>>..>>.>...>>>>>>>...<<<<<<<<<.<......>
>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<<<<<.....>>>>
>.>>>>....<<<......>>>...<<<<<<<...<<<(((.......<<
<<<......<..........<<<.<<<<<<<<<<........<<<<.<<.
...<<.........>>....>>.........<<......>>...>>>>..
..>>>>>>>>>>>>>..........))).>....>>>>>..>>>...>>>
>>>><<<<<<.<<<........}...........]].]]...........
..>>>>>>>>>....>>>>>>...<<<<<<<<.<<......<<<<.<<<<
....>>>>>>>>...>>>>>>>>>>........>>>>>><<.....<<..
...<<<<<<.......>>>>>>.<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
........<<.<<<<<<<<.....>>>>>>>>>>......>>....>>>.
.<<<<<........>>>>>))<<<<....<<<<<..<<<<<<<<....>>
>>>>>>..<<<....>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<
<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<<<.....<<<...
..>>>.....>>>>>>>>..>>>>>.....<<<<<<<.......>>>>>>
>>>>>...>>>>>>>>>>>>.........>>>>>>>..>..........>
>.>>>.>>>>.>>>..>>.....<<......>>>>>>>.<<<<.....>>
>>..>>>....<<<<.....<<<<<<<.<<<..............<<<<<
....<....>.....>>>>><<.<<<<<<<<<<<.>>>>>..>>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....
<<<.....<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<....
...>>>>>>........<...........<<<<<.......>>>>>....
.>.....>>....>>.>>>>>>>....>>>.>>>>>>>..>>>>>>....
>>....<<<<<........>>>>>....<<<<<..<<<.<<......<<<
<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<<<<<<<<.<<<<.......
...<<<.<..<<<<..>>>>............>.>>>.>>>>.>>.>>>>
>>>>>>...<<<<<.....<<<<..<<...<<<>>.>..>>.........
.....<<<<<<<<<..>>>>>>>>>..>>>>..>>>>>....>>>>>>>>
>..>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<
<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>..
..>>>>>>>...<<<..<<<<.......>>>>..>>><<<<....>>>>>
>)))].....<<<<<<<..<<<..)))))).>>>>>>>>>>.........
>>>>..>>>>>>.......<<.<...<<<<<<...<<.......>>...>
>>>>>....>.>.>.......<.<<<.<<<<<<..<.<.<<<<<......
>>>>>...>.>.<<<.........>>>.....>>>>>..>...>>>..>.
...<<<<<<<....>>>.>>>>....>>..>>>.>>>>>.....<<<<<<
.<..<<<..<.<<<<<.................>>>>>.><<<<....<<
<<....<<.<<<<<<.]]]..>>>>..>>.>>...>>>>...>>>><<<<
<...<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>.<
<<<<<...<<<<....<<<........<<<<<..<<<<<.>>>>>.>>>>
>.......>>>...<<<..<<<<....<<....<.............>..
..>>...>>>>..>>>..............>>>>....>>>>>>.<<<<<
.<.............>....<<.<<<<<<<.<<.....<<<<.<..<<<<
<.>>>>>..>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6p5i The Israeli acute paralysis virus IRES captures host ribosomes by mimicking a ribosomal state with hybrid tRNAs.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): Y11 G16 G18 R19 A34 K35 S38 R44 I49 N50 W51 R56 K61
• Binding residue (residue number reindexed from 1): Y11 G16 G18 R19 A34 K35 S38 R44 I49 N50 W51 R56 K61

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0010458 exit from mitosis
• GO:0021554 optic nerve development
• GO:0031290 retinal ganglion cell axon guidance
• GO:0060041 retina development in camera-type eye

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6p5i, PDBe:6p5i, PDBj:6p5i
• PDBsum: 6p5i
• PubMed: 31609474
• UniProt: G1SE28|RL24_RABIT Large ribosomal subunit protein eL24 (Gene Name=RPL24)