Structure of PDB 7mpj Chain AV Binding Site BS01

Receptor Information

>7mpj Chain AV (length=136) Species: 4932 (Saccharomyces cerevisiae)

SGNGAQGTKFRISLGLPVGAIMNCADNSGARNLYIIAVKGSGSRLNRLPA
ASLGDMVMATVKKGKPELRKKVMPAIVVRQAKSWRRRDGVFLYFEDNAGV
IANPKGEMKGSAITGPVGKECADLWPRVASNSGVVV

Ligand information

>7mpj Chain A1 (length=3137)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaggccagcaucag
uuuugguggcaggauaaauccauaggaauguagcuugccucgguaaguau
uauagccugugggaauacugccagcugggacugaggacugcgacguaagu
caaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacc
aaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcgua
augaaagugaacguagguuggggccucgcaagaggugcacaaucgaccga
uccugaugucuucggauggauuugaguaagagcauagcuguugggacccg
aaagauggugaacuaugccugaauagggugaagccagaggaaacucuggu
ggaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguau
aggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguu
ucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaaug
auuagagguuccggggucaccuugaccuauucucaaacuuuaaauaugua
agaaguccuuguuacuuaauugaacguggacauuugaaugaagagcuuuu
agugggccauuuuugguaagcagaacuggcgaugcgggaugaaccgaacg
uagaguuaaggugccggaauacacgcucaucagacaccacaaaagguguu
aguucaucuagacagccggacgguggccauggaagucggaauaggagugu
guaacaacucaccggccgaaugaacuagcccugaaaauggauggcgcuca
agcguguuaccuauacucuaccgucaggguugauaugaugcccugacgag
uaggcaggcguggaggucagugacgaagccuagaccguaaggucgggucg
aacggccucuagugcagaucuuggugguaguagcaaauauucaaaugaga
acuuugaagacugaaguggggaaagguuccacgucaacagcaguuggacg
uggguuagucgauccuaagagauggggaagcuccguuucaaaggccugau
uuuaugcaggccaccaucgaaagggaauccgguuaagauuccggaaccug
gauauggauucuucacgguaacguaacugaauguggagacgucggcgcga
gcccugggaggaguuaucuuuucuucuuaacagcuuaucaccccggaauu
gguuuauccggagauggggucuuauggcuggaagaggccagcaccuuugc
uggcuccggugcgcuugugacggcccgugaaaauccacaggaaggaauag
uuuucaugccaggucguacugauaaccgcagcaggucuccaaggugaaca
gccucuaguugauagaauaauguagauaagggaagucggcaaaauagauc
cguaacuucgggauaaggauuggcucuaagggucggguacgaucaacuua
gaacugguacggacaaggggaaucugacugucuaauuaaaacauagcauu
gcgauggucagaaagugauguugacgcaaugugauuucugcccagugcuc
ugaaugucaaagugaagaaauucaaccaagcgcggguaaacggcgggagu
aacuaugacucucuuaagguagccaaaugccucgucaucuaauuagugac
gcgcaugaauggauuaacgagauucccacugucccuaucuacuaucuagc
gaaaccacagccaagggaacgggcuuggcagaaucagcggggaaagaaga
cccuguugagcuugacucuaguuugacauugugaagagacauaguuucuu
uacuuauucaaugaagcggagcuggaauucauuuuccacguucuagcauu
caaggucccauucggggcugauccggguugaagacauugucaggugggga
guuuggcuggggcggcacaucuguuaaacgauaacgcagauguccuaagg
ggggcucauggagaacagaaaucuccaguagaacaaaaggguaaaagccc
ccuugauuuugauuuucagugugaauacaaaccaugaaaguguggccuau
cgauccuuuagucccucggaauuugaggcuagaggugccagaaaaguuac
cacagggauaacuggcuuguggcagucaagcguucauagcgacauugcuu
uuugauucuucgaugucggcucuuccuaucauaccgaagcagaauucggu
aagcguuggauuguucacccacuaauagggaacgugagcuggguuuagac
cgucgugagacagguuaguuuuacccuacugaugaauguuaccgcaauag
uaauugaacuuaguacgagaggaacaguucauucggauaauugguuuuug
cggcugucugaucaggcauugccgcgaagcuaccauccgcuggauuaugg
cugaacgccucuaagucagaauccaugcuagaacgcggugauuucuuugc
uccacacaauauagauggauacgaauaaggcguccuuguggcgucgcuga
accauagcaggcuagcaacggugcacuuggcggaaaggccuugggugcuu
gcuggcgaauugcaaugucauuuugcguggggauaaaucauuuguauacg
acuuagauguacaacgggguauuguaagcaguagaguagccuuguuguua
cgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<<....>>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<..<<.<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>......
.>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.<....<<<<<<<.<<<
<<<<<<<<<<<<.....<<<<<<<<<......<<<<........>>>>..
.....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>
>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><<<
<<<...<<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...
<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<
<<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>>>.<<.<
..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>
.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>
...<<.......>>......>>>>>>...>..>.>>..<<<<<<....<.
<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<....<<
<....<<<.................>>>....>>>..>.>>.....>>>>
>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>>.>>>>>>>>
.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<
<<<<<<..<<<<(((.....<<<<<<......<..........<<<.<<<
<<<<<<<.........<<<.............................<.
.......>...>>>.....>>>>>>>>>>>>>..........))).>...
.>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>>>>..
..>>>>>>..<<<<<<<..<<......<<<<.<<<<....>>>>>>>>..
.>>.>>>>>>>........>>>>>><<.....<<.....<<<<<<.....
..>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>
>>>....((<<<<<<...<<....<<<....>>>.........<<<....
........>>>.....>>....>>...<<<<<........>>>>>))<<<
<.<<<<<<<<....<<<....>>>.......<<<<<<...<<<<.<<<<<
<<.<<<<<<<<<<..[[[..>>>>>><...<<<<<....<<<<<<....<
<<.....>>>....>>>>>>.....>>>>>....><<<<<<<......>>
>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>.........>>
>>..>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....
>>>>..>>>>..<<<<.....<<<<<<<.<<.........<.....<<<<
<...............>>>>><<.<<<<<<<<<........>>>..>>>>
>>.>>............<<<<<<<<<..<<<.........>>>..<<<<<
<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<
.....<<.....<<<<....>>>>.....>>..>>>>..>>>>><<<<<<
.......>>>>>>........<...........<<<<<.......>>>>>
.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>
....>>....<<<<<........>>>>>....<<<<<..<<<.<<.....
.<<<<.<<<<<<..<<<<<.<<<<<<<<..<<<<<.............>>
>>>...<<<<<.....<<<<..<<<..........>>>............
...<<<<<<....>>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>
>>...<<<.....>>>..<<<<<<...........>>>>>>..<<...<<
<<<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>
>>>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))..
....<<<<<<<..<<<<.))))))>>>>>>>>>>>.........>>>>..
>>>>>>.......<<.<.<.<<<<<<...<<.......>>...>>>>>>.
..>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>
>...>.<<<.........>>>.....>>>>>..>...>>>.>>....<<<
<<<<....>>>.>>>>....>>..>>>.>>>>>....<<<<<<<<..<<<
<...<<<<<<...............>>>>>>..<<<<<...<<<<....<
<<<<<<<<<]]].>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<
<<...........>>>>>...>>>>.>>>>....>>>>>>>>.......<
<<<<<.<<<.<<....<<..........<<<<<<.....>>>>>>.....
.>>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>>>.
>>>>>>.............>>.>>>.>>>>>>..<<<<<.<.........
....>.....<<<<<<<<<.<<<....<<<<....<<<<<......>>>>
>...>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mpj CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations.
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): T9 K10 F11 R12 S14 G16 P18 A21 I22 I37 A38 K40 G41 S44 R45 L46 N47 R48 L49 M59 T61 K63 K71 V73 K83 R86 R88 F92 N98
• Binding residue (residue number reindexed from 1): T8 K9 F10 R11 S13 G15 P17 A20 I21 I36 A37 K39 G40 S43 R44 L45 N46 R47 L48 M58 T60 K62 K70 V72 K82 R85 R87 F91 N97

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7mpj, PDBe:7mpj, PDBj:7mpj
• PDBsum: 7mpj
• PubMed: 35489333
• UniProt: P0CX41|RL23A_YEAST Large ribosomal subunit protein uL14A (Gene Name=RPL23A)