Structure of PDB 8fmw Chain AU Binding Site BS01

Receptor Information

>8fmw Chain AU (length=115) Species: 224326 (Borreliella burgdorferi B31)

LVNRRYTAKGKNLPSSPKKVRPIADNIRGESYIKAIAVLCSMPNKGAKLL
EKVVKSAASNAMYHNKNLSEDMIFVKTVMVDDGRRRKKIWPRARGRADRL
VNRNCHIFVEVDEKK

Ligand information

>8fmw Chain AA (length=2929)

uggucaaaguaauaagagucuauggugaaugccuaggagcuuuaaggcga
agaaggucgugguaagcugcgaaaagcuugggggagaagcaaacauuuau
ugaucccaagauuaccgaauggaguaauccagcuagcaagaugcuagcua
ucuauuauuuaaauaauagaggcgauaccaggggaagugaaccaucuaag
uacccugaggaaaagaaaucaaagagauucccuuaguaguggcgagcgaa
aagggaguagcccaaacuuuaaaugugucaagcugcagagcguugcauuu
augggguuguaggacguuuaggcuuagucuguaauaagcaaaaaaguuac
aaaauauuuauauagaagaauaaucuggaaaguuuaaccaaagaagguga
uaguccuguaauuuaaauguaaauaucuuuuuaaaauguuccugaguagg
acgaggcacgagaaaccuuguuugaagcuggggagaccacucuccaaggc
uaaauacuagaaagcuaccgauagagaagaguaccgugagggaaagguga
aaagaaccccgggaggggagugaaauagaacugaaaccguagacuuacaa
gcagucaaagccguaauuuauugcggugauggcgugccuuuugcauaaug
aaccugcgaguuaucaugucuagcaagauuaaagcauagaagugcuggag
ucgaagcgaaagcgagucuuaaaagggcgauuuaguuagaugugguagac
ccgaagccgagugaucuauuuauggccaggcugaagcuuggguaaaacca
aguggagggccgaacucuagucuguuuaaaaaggcagggaugagcuguga
auaggagugaaaggcuaaacaaacucggagauagcugguucuccccgaaa
uggauuuaaguucagccuuauuuuaguuuaauagagguagagcacuaauu
gagcuagggccugucaaaggguaccaaacucaguuaaacuccgaaugcua
uuaaaugaugaauaggagugagacuaugggcgauaagguucauagucgag
agggaaacaacccagaccaacagcuaaggucucaaaaauguguuaagugg
aaaaggagguuuagguacguaaacagccaggagguuggcuuagaagcagc
cauaccuuuaaagagugcguaauagcucacuggucgaguacuuaagcgcc
gauaauguaacggggcuaaacacauuaccgaagcuuuggaucuuaacgaa
aguuaagaugguaggggagcguucuguaagccagagaaguuaagcuggaa
aguuugauggagguaucagaagugagaaugcagguaugaguaacgaaaaa
augggugagauucccauucgccgaaaaccuaagguuuccuggguaaaggu
cgucuucccaggguuagucggccccuaaggcaaagcugaaaaguguaguc
gaugggaaacggguuaauauucccguaccucuuauaguuucgauggagug
acgcaugagguuaacuacugcuaggcgaugguuguccuaguuuaagcauu
aaggcgaugaucuuaauaggaaaauccguuaagagagcuaagaugugaug
augagugcuauuuagguagcaugaaauguagguagucaaggugccaagaa
auagcuucuaagguuaggcuauaagggaccguaccgcaaaccgacacagg
uaggugggaugaaaauucuaaggcgcgcgagagaauccacguuaaggaac
ucugcaaaauacguacguaacuucgggauaaguacgaccuaagcaauuag
guagcauaaaaaugguccaaacgacuguuuaccaaaaacacaggucucug
caaaucuguaaagagaaguauagggacugacaccugcccggugcuggaag
guuaagaggagauguuaguuuaugcgaagcauugaauuuaagccccagua
aacggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgg
guaaguuccgacccgcacgaaugguguaacgauuuggacgcugucucaac
guggagcucggugaaauugaaguaucggugaagaugccgauuacuugugg
uuagacggaaagaccccgugaaccuuuacuauagcuugguauugagauuu
gauuaaauauguguaggauaggugggagacuuugaagcuaucucgucagg
gguaguggagucaaucuugaaauaccacccuuguuuaauuagguuucuaa
cuuauagaaauaugaggagagugccagguggguaguuugacuggggcggu
cgccuccuaaagaguaacggaggugcgcaaagguuaccuuagagugguug
gaaaucacucuguaaguguaaaggcauaagguagcuuaacuguaagacug
acaagucgaacagauacgaaaguaggucuuagugaucuggcgguggcaag
uggaagcgccgucacuuaacgaauaaaagguacuccggggauaacaggcu
uauccuucccaagaguucacaucgacggaaggguuuggcaccucgauguc
ggcucaucgcauccuagggcuggagcagguccuaaggguauggcuguucg
ccauuuaaagcgguacgcgagcuggguucagaacgucgugagacaguuug
gucccuaucugccacaagcguuggauauuugagaggagcuaucuuuagua
cgagaggaccgagauggacgaaccucuagugugccaguuauccugccaag
gguaagugcuggguagcuacguucggaaaggauaaccgcugaaagcaucu
aagugggaagccuuccucaagaugagauauccuuuaaggguccuggaaga
auaccagguugauagguuagaaguguaaguauagcaauauauuaagcuga
cuaauacuaauuacccguaucuuuggcca

.<<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<.<<<<..
....<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>..
...>>>>>>>...>>>....<<<....>>><<<<<<<.....>>>>>>>.
<<<<<<..<..>..>>>>>>.>>....<<<<<<.....<<....>>....
..>>>>>.>.........<<...>>..<<<<<<.....<<.....>>...
.>>>>>>..<<<....<<.<<<<<<<.......<<.<<<.....>>>>>>
>.>>.......<<<<.[....<<<<<.>>>.>>.>>>>>.<<<<......
..<.<<<<.<<<<.<<....<<<.<......>.>>>((......<<<...
..))>>>....>>...>>>>>>.>>>.>>>>...]..>>>>.......<<
<<<<<<.........>>>>>>>>......<<<<<..((((>>>>>..>>>
......>>..>>>>>>.>>..............<<....>>....>>...
......<<<<....>>>>.................>>>>>>>>>>>....
...<<<<..<<<<<<<<..>>>>>>>>..>>>>...<.<........>.>
.<<<<<<....<<<<<<<<<<<<<..<<<...<<<<<....>>>>>...>
>>...<<....>>..<<<<.....>>>>......>>>>>>>>>>>>>..<
<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>
>>>.....>>>...<<<<.<<<<........>>>>>>>>....>>>>>>>
>>...<<<.....>>>......>>>>>>...>..>.>>..<<<<<<....
<<<<<....>>>>><<<<<<<<<..<<<<<<<<<.....<<<...<<<<<
<<<...<<<<<<......>>>>.>>...>>>>>>>>..>>.>.....>>>
>>>>>>...>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>...
.<<<......>>>...<<<<.<<<...<<<((....<<<<<<<.......
....<<..<<<<<<<<<<.......<<<<<.<<<..<<<.........>>
>...>>>.........<<......>>...>>>>>...>>>>>>>>>>.>>
............)).....>>>>>>>>>>...>>>>>>><<<<<<<<...
.>>>>>>>>....>>>>>><.<<<<<...<<<......<<<<<<<<....
>>>>>>>>...>>>..>>>>>..>.....>>>>>><......<<.....<
<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<...<<..
...>>.>>>>>>....((<<<.<<<...<<<...<<<....>>>...>>>
...>>>..<<<<<........>>>>>)).<<<<<<<<<.<<...<<<<<.
..<<<<..<<<...<<<<.<<<<<<...[[[[...>>>>>><<..<<<<<
..<<<......<<<<<............>>>>>...>>>..>>>>>...>
>...<<<<<<<....>>>>>>>.....>>>>...>>>...>>>>......
...>>>>>...>>....>>>>>>>>>..>>>.>>>.....<<......>>
>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<<.<<....
.....<.....<<<<<.....<..>......>>>>><<<<<<....>>>>
>>............<<<<<<<<<..<<<.........>>>..<<<<<<<.
....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<
<<<.<<<...<<<<<..<<....>>...>>>>>...>>>.>>>>>>>>>.
.>>>>><<<<<<.......>>>>>>........<...........<<<<<
<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>
>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<
<.<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<<<
<<<<<<<<<...........<<<<<..<<<......<<<<<<<<....>>
>>>>>>...>>>............>>>>>..>>>>>>>>>>>>>>>>...
<<<<<<....>>>>>>....>>>>>>>>>..>>>>>..<<<<.....>>>
><<<<<<...........>>>>>>..<<...<<<<<<<.<<<<<<<<...
....>>>>>>>>...<<.[.(((>>....>>>>>>>...<<<<..<<<<.
...>>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.<<..)
)))..>>>>>>>>>.............>>>>..>>>>>>.......<<<<
.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>.......<<
<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<.....>
>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.
...>>..>>>>>>>>>>>....<<<<<<<...<<<<<.<<<<<<<.....
..........>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<]]]]>>
>>>>...>>>>>...>>>>.>>>>>...<<<....<<<<<..........
.>>>>>....>>>>>>>>.......>>>>>>>.....<<<<<<<<<....
...>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..
>>>>>.>>>...>>>>....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fmw The structure of a hibernating ribosome in a Lyme disease pathogen.
• Resolution: 2.86 Å
• Binding residue (original residue number in PDB): R5 R6 T8 K10 K12 S17 K19 K20 R22 N45 K46 K53 S60 N61 H65 M80 D82 D83 R86 W91 P92 R93 A94 R95 G96 R97 A98 D99 L101 V102 N103
• Binding residue (residue number reindexed from 1): R4 R5 T7 K9 K11 S16 K18 K19 R21 N44 K45 K52 S59 N60 H64 M79 D81 D82 R85 W90 P91 R92 A93 R94 G95 R96 A97 D98 L100 V101 N102

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fmw, PDBe:8fmw, PDBj:8fmw
• PDBsum: 8fmw
• PubMed: 37907464
• UniProt: P94272|RL22_BORBU Large ribosomal subunit protein uL22 (Gene Name=rplV)