Structure of PDB 8hkx Chain AS7P Binding Site BS01

Receptor Information

>8hkx Chain AS7P (length=193) Species: 330779 (Sulfolobus acidocaldarius DSM 639)

ENIEVSNLNVKVFGKWDTKVEVRDPSLKKYIDLMSIYLPHTGGRHEHRRF
GKSRIPIVERLINNLMRPGRNKGKKMLAYNIVKTTFDIIAVKTGQNPIQV
LVRAIENAAPREEVTRIMYGGIVYYVAVDVAPQRRVDLALRHLVTGASEA
SFNNPKPIEEALAEEIIAAANNDNKSVAIRKKEEIERIALSSR

Ligand information

>8hkx Chain A16S (length=1491)

auuccgguugauccugccggacccgaccgcuaucgggguagggauaagcc
augggagucuuacacucccggguaagggaguguggcggacggcugaguaa
cacguggcuaaccuacccucgggacggggauaaccccgggaaacugggga
uaauccccgauagggaaggaguccuggaaugguuccuucccuaaagggcu
auaggcuauuucccguuuguagccgcccgaggauggggcuacggcccauc
aggcugucggugggguaaaggcccaccgaaccuauaacggguaggggccg
uggaagcgggagccuccaguugggcacugagacaagggcccaggcccuac
ggggcgcaccaggcgcgaaacguccccaaugcgcgaaagcgugagggcgc
uaccccgagugccuccgcaggaggcuuuuccccgcucuaaaaaggcgggg
gaauaagcggggggcaagucuggugucagccgccgcgguaauaccagcuc
cgcgaguggucggggugauuacugggccuaaagcgccuguagccggccca
ccaagucgccccuuaaaguccccggcucaaccggggaacugggggcgaua
cuggugggcuagggggcgggagaggcgggggguacucccggaguaggggc
gaaauccuuagauaccgggaggaccaccaguggcggaagcgccccgcuag
aacgcgcccgacggugagaggcgaaagccggggcagcaaacgggauuaga
uaccccgguagucccggcuguaaacgaugcgggcuaggugucgaguaggc
uuagagccuacucggugccgcagggaagccguuaagcccgccgccugggg
aguacggucgcaagacugaaacuuaaaggaauuggcgggggagcaccaca
agggguggaaccugcggcucaauuggagucaacgccuggaaucuuaccgg
gggagaccgcaguaugacggccaggcuaacgaccuugccugacucgcgga
gaggaggugcauggccgucgccagcucguguugugaaauguccgguuaag
uccggcaacgagcgagacccccaccccuaguugguauucuggacuccggu
ccagaaccacacuagggggacugccggcguaagccggaggaaggaggggg
ccacggcaggucagcaugccccgaaacccgggccgcacgcggguuacaau
ggcagggacaacgggaugcuaccucgaaagggggagccaauccuuaaacc
cugccgcaguugggaucgagggcugaaacccgcccucgugaacgaggaau
cccuaguaaccgcgggucaacaacccgcggugaauacgucccugcuccuu
gcacacaccgcccgucgcuccacccgagcgagaaaggggugaggucccuu
gcgauaagugggggaucgaacuccuuucccgcgaggggggagaagucgua
acaagguagccguaggggaaccugcggcuggaucaccucau

...<<<<<...((((>>>>>.<<<<<<<<<.....<<<.<<......<<<
.<<<..<<<..<<<<<<<<......>>>>>>>>>>>......<<......
..<<<<<<<..<<...<<<<<<<.<<<<<....<<<<<......>>>>>.
....>>>>>..<<<<<<<<<<.<<......>>>>>>>>>>>>...<<.<<
<<<<............>>>>>>>>.>>>>>>>..>>>>>>>>>.<<<...
.<<...<<<<<<<<.......>>>>>>>>.>>......>>>..<<<<<<<
<....>>>>...>>>>.>>.<<<<<............>>>>>.<<<<<..
>>>>>...>>>>>>.........<<<....<<<<....>>>>..>>>..>
>..>>>....<<<<<<...>>>>>>...<<<<<<<.........>>>>>>
>.....<<<<<((......<<<<.....<<..))>>.......>>>>.>>
>>>..>>>>>>>>>..........<<<((.....<<<<...<<<.<<<<<
<<.<<<<<<<<<......<<<<<<<.....>>>>>>>....>>>>>>>..
>>>>>>>>>...<<<<<<.....<<<<<<<<<..<<<<<<<....<<<..
....>>>.......>>>>>>>..>>.......<<....>>.>>>>>>>..
..>>>.>>>...>>>...>>>>...<<<<<<<...<<...<<<<......
...>>>>...>>>>>>>>>.........<<<<<<..<<<<<<<<<<<<<<
.....>>>>>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<.
.....<<<<....>>>>....>>>..))))...<<<..<<<<<<<..<<.
<<<<<<..<<<<<<<<<<......<<........>>..........<<<<
......<<<<<<......<<<.<<<.......>>>.>>>...>>.>>>>.
..<<.<<<..<<<<<<<<.....<<<<<<<<<....>>>..<<<<.....
.>>>>..>>>>>>....<<<<<.<<<<<<<..<...<.<<<<<<....>>
>>>>.>...>.>>>>>>>.....<<<<<....>>>>>........>>>>>
.....>>.>>>...>>>>>>>>.....>>>>...>>.>>>>>>>>.....
<<<<<<<......<<..<<..<<<<....>>>>..>>....>>.....>>
>>>>>......<....<<<<<<<........>>>>>>>....>.....>>
>>>>....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>.
>>.>.....<..<<.<.<<<<.<<<..<<<.<<<<<<<<<..<<<<<<<<
<<.....>>>>>>>>>>..>>>>>>>>>.>>>..>>>.>>>>..>.>>..
.>.....<<<<<<<<<....>>>>>>>>>............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8hkx Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome
• Resolution: 3.14 Å
• Binding residue (original residue number in PDB): G44 H47 E48 H49 R50 R51 N65 L67 R69 P70 G71 N73 K74 G75 K76 K77 M78 R118 D139 R143 S153 F154 N155 I160
• Binding residue (residue number reindexed from 1): G42 H45 E46 H47 R48 R49 N63 L65 R67 P68 G69 N71 K72 G73 K74 K75 M76 R116 D137 R141 S151 F152 N153 I158

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8hkx, PDBe:8hkx, PDBj:8hkx
• PDBsum: 8hkx
• PubMed: 37604686
• UniProt: P17198|RS7_SULAC Small ribosomal subunit protein uS7 (Gene Name=rps7)