Structure of PDB 7toq Chain AS11 Binding Site BS01

Receptor Information

>7toq Chain AS11 (length=143) Species: 9986 (Oryctolagus cuniculus)

DIQTERAYQKQPTIFQNKKRVKLPRYYKNIGLGFKTPKEAIEGTYIDKKC
PFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMS
VHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKK

Ligand information

>7toq Chain A18S (length=1690)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
cccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggu
agucgccgugccuaccauggugaccacgggugacggggaaucaggguucg
auuccggagagggagccugagaaacggcuaccacauccaaggaaggcagc
aggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaaua
acaauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaa
auccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcg
auaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcu
cguaguuggaucuugggagcggccguccccugcggcccgaagcguuuacu
uugaaaaaauuagaguguucaaagcaggccgccuggauaccgcagcuagg
aauaauggaauaggaccgcgguucuauuuuguugguuuucggaacugagg
ccaugauuaagagggacggccgggggcauucguauugcgccgcuagaggu
gaaauucuuggaccggcgcaagacggaccagagcgaaagcauuugccaag
aauguuuucauuaaucaagaacgaaagucggagguucgaagacgaucaga
uaccgucguaguuccgaccauaaacgaugccgacuggcgaugcggcggcg
uuauucccaugacccgccgggcagcuuccgggaaaccaaagucuuugggu
uccggggggaguaugguugcaaagcugaaacuuaaaggaauugacggaag
ggcaccaccaggaguggagccugcggcuuaauuugacucaacacgggaaa
ccucacccggcccggacacggacaggauugacagauugauagcucuuucu
cgauuccgugggugguggugcauggccguucuuaguugguggagcgauuu
gucugguuaauuccgauaacgaacgagacucuggcaugcuaacuaguuac
gcgaccccggucggcguaacuucuuagagggacaaguggcguucagccac
ccgagauugagcaauaacaggucugugaugcccuuagauguccggggcug
cacgcgcgcuacacugacuggcucagcgugugccuacccuacgccggcag
gcgcggguaacccguugaaccccauucgugauggggaucggggauugcaa
uuauuccccaugaacgaggaauucccaguaagugcgggucauaagcuugc
guugauuaagucccugcccuuuguacacaccgcccgucgcuacuaccgau
uggaugguuuagugaggcccucggaucggccccgccggggugcccuggcg
gagcgcugagaagacggucgaacuugacuaucuagaggaaguaaaagucg
uaacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<......<<....<<<...........<<.....<<..<<...
..>>..>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<<.>>>>>>>>....>>>...>>....>>>.<<<....<<<....<<<<<
<<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>.
...>>>.>>.<<.<<<..........>>>.>>.<.<<....>>.>...>>
>>>>.........<<<....<<<<...>>>>..>>>..>...>>.>....
.<<<<<<<<<.<<<....>>>..>>>>>>.>>>......<<..<......
.....>..>>.........<<<<<((......<<<<.....<<..))>>.
......>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<
<...<<<.<<....<<<.<<<..>>>.>>><<<........>>>.<<<<<
<.<.......<<...<.......>..<<<.>>>....>>......>.>>>
..>>>........<.<..<<<<<<<...............>>>>>>>..>
.>....>>....<<<<<<..<...<<<<..<<<.<<<<<<<<...<<<..
....>>>......>>>>>>>>.>>>.......<<....>>...>>>>..>
..>>>.>>>...>>>...>>.>....<<<<<<<...<...<<<<......
...>>>>...>>>>>>>>..........<<<.<<.<<<..<.<<<<<<.<
<<<<....>>>>>>>>>>>.>..>>>...<<..))>>...>>.....>>>
.>.>.<<<......<<<<....>>>>....>>>..)))).]<<<<<.<<<
<<<<..<<..<<<<<.<<<<.<<<<<<......<<........>>.....
.....<<<<<.<....<<<<<<.......<<.<<<........>>>.>>.
....>>>>>>.<.<<.<<<..<<.<<<<<<....<<<.<<<<<....>>>
...<<<......>>>...>>.>>>....<<<...<...<<<<..<<<<<<
<<<<<<..>>>>.>>>>>>>>..>>>>..>.....<<<<<.....>>>>>
........>>>....>>>.>>>.....>>>>>>>>....>.>>>>>...>
>.>>>>.>>>.>...<<.<<<........<.......<<<.<<<<....>
>>>.>>>....>.........>>>.>>......<...<.<<<<<......
....>>>>>>....>.....>>>>>.....<<<<<<<<.......>>>>>
>>>......>>...>>>>>>>>>>.>>....<..<<.<..<<<<.<<...
.<<<<<<<<.<<<..<<<<.<<...<<<<<<<<<<<<<<<..>>>>>>>>
>.>.>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>>...>.>>
...>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7toq Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): E7 R8 A9 T15 F17 Y36 N39 I40 L42 K59 N65 S67 I68 R69 G70 R71 K79 K81 M82 R89 R90 D91 Y92 R97 K98 Y99 R101 K104 R105 H106 K107 N108 E130 R132 P133 L134 S135 K136 R139 F140
• Binding residue (residue number reindexed from 1): E5 R6 A7 T13 F15 Y26 N29 I30 L32 K49 N55 S57 I58 R59 G60 R61 K69 K71 M72 R79 R80 D81 Y82 R87 K88 Y89 R91 K94 R95 H96 K97 N98 E120 R122 P123 L124 S125 K126 R129 F130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7toq, PDBe:7toq, PDBj:7toq
• PDBsum: 7toq
• PubMed: 35589706
• UniProt: G1TRM4|RS11_RABIT Small ribosomal subunit protein uS17 (Gene Name=RPS11)