Structure of PDB 7n8b Chain AR Binding Site BS01

Receptor Information

>7n8b Chain AR (length=188) Species: 4932 (Saccharomyces cerevisiae)

ANLRTQKRLAASVVGVGKRKVWLDPNETSEIAQANSRNAIRKLVKNGTIV
KKAVTVHSKSRTRAHAQSKREGRHSGYGKRKGTREARLPSQVVWIRRLRV
LRRLLAKYRDAGKIDKHLYHVLYKESKGNAFKHKRALVEHIIQAKADAQR
EKALNEEAEARRLKNRAARDRRAQRVAEKRDALLKEDA

Ligand information

>7n8b Chain A1 (length=3156)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacuggccagcaucaguuuugguggcaggauaaauccauaggaaugu
agcuugccucgguaaguauuauagccugugggaauacugccagcugggac
ugaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccgc
ccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuuggg
uguaaaacccauacgcguaaugaaagugaacguagguuggggccucgcaa
gaggugcacaaucgaccgauccugaugucuucggauggauuugaguaaga
gcauagcuguugggacccgaaagauggugaacuaugccugaauaggguga
agccagaggaaacucugguggaggcucguagcgguucugacgugcaaauc
gaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagua
gcugguuccugccgaaguuucccucaggauagcagaagcucguaucaguu
uuaugagguaaagcgaaugauuagagguuccggggucaccuugaccuauu
cucaaacuuuaaauauguaagaaguccuuguuacuuaauugaacguggac
auuugaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcg
augcgggaugaaccgaacguagaguuaaggugccggaauacacgcucauc
agacaccacaaaagguguuaguucaucuagacagccggacgguggccaug
gaagucggaauaggaguguguaacaacucaccggccgaaugaacuagccc
ugaaaauggauggcgcucaagcguguuaccuauacucuaccgucaggguu
gauaugaugcccugacgaguaggcaggcguggaggucagugacgaagccu
agaccguaaggucgggucgaacggccucuagugcagaucuuggugguagu
agcaaauauucaaaugagaacuuugaagacugaaguggggaaagguucca
cgucaacagcaguuggacguggguuagucgauccuaagagauggggaagc
uccguuucaaaggccugauuuuaugcaggccaccaucgaaagggaauccg
guuaagauuccggaaccuggauauggauucuucacgguaacguaacugaa
uguggagacgucggcgcgagcccugggaggaguuaucuuuucuucuuaac
agcuuaucaccccggaauugguuuauccggagauggggucuuauggcugg
aagaggccagcaccuuugcuggcuccggugcgcuugugacggcccgugaa
aauccacaggaaggaauaguuuucaugccaggucguacugauaaccgcag
caggucuccaaggugaacagccucuaguugauagaauaauguagauaagg
gaagucggcaaaauagauccguaacuucgggauaaggauuggcucuaagg
gucggguacgaucaacuuagaacugguacggacaaggggaaucugacugu
cuaauuaaaacauagcauugcgauggucagaaagugauguugacgcaaug
ugauuucugcccagugcucugaaugucaaagugaagaaauucaaccaagc
gcggguaaacggcgggaguaacuaugacucucuuaagguagccaaaugcc
ucgucaucuaauuagugacgcgcaugaauggauuaacgagauucccacug
ucccuaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcag
aaucagcggggaaagaagacccuguugagcuugacucuaguuugacauug
ugaagagacauaguuucuuuacuuauucaaugaagcggagcuggaauuca
uuuuccacguucuagcauucaaggucccauucggggcugauccggguuga
agacauugucagguggggaguuuggcuggggcggcacaucuguuaaacga
uaacgcagauguccuaaggggggcucauggagaacagaaaucuccaguag
aacaaaaggguaaaagcccccuugauuuugauuuucagugugaauacaaa
ccaugaaaguguggccuaucgauccuuuagucccucggaauuugaggcua
gaggugccagaaaaguuaccacagggauaacuggcuuguggcagucaagc
guucauagcgacauugcuuuuugauucuucgaugucggcucuuccuauca
uaccgaagcagaauucgguaagcguuggauuguucacccacuaauaggga
acgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacug
augaauguuaccgcaauaguaauugaacuuaguacgagaggaacaguuca
uucggauaauugguuuuugcggcugucugaucaggcauugccgcgaagcu
accauccgcuggauuauggcugaacgccucuaagucagaauccaugcuag
aacgcggugauuucuuugcuccacacaauauagauggauacgaauaaggc
guccuuguggcgucgcugaaccauagcaggcuagcaacggugcacuuggc
ggaaaggccuugggugcuugcuggcgaauugcaaugucauuuugcguggg
gauaaaucauuuguauacgacuuagauguacaacgggguauuguaagcag
uagaguagccuuguuguuacgaucugcugagauuaagccuuuguugucug
auuugu

........................<<<<<<.....<......>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<.<<.<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>......
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.<...............
........<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<<.....
.<<<<........>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>>
>>........<<<....>>>.....>>>>>>>....>>>>>>.>>>>>..
<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<<
<.....>>>>...>>>...<<....>>.......<<<<<..<<<<<....
>>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....
>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>
>>.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>.
.>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<
<<<<<.....<<<....<<<....<<<.................>>>...
.>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.>>>>
.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>.
...<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.....
<<..........<<<.<<<<<<<<<<.........<<<............
..............................>>>.....>>>>>>>>>>>>
>..........))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<.
........>>>>>>>>>....>>>>>><.<<<<<<<<.<<......<<<<
.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<....
.<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<
<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<....
>>>........<<<<............>>>>....>>....>>...<<<<
<........>>>>>))<<<<.<<<<<<......<<<....>>>.......
<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..[[[..>>>>>><...<
<<<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>.
...><<<<<<<......>>>>>>>>>>>...>>>>>>>.>>>>.......
..>>>>>>............>>.>>>>>>>>>>>>..>>.....<<....
..>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<
.........<.....<<<<<...............>>>>><<.<<<<<<<
<<<<....>>>>>..>>>>>>.>>............<<<<<<<<<..<<<
.........>>>..<<<<<<<....<<<<.....>>>>......>>>>>>
>...<<..<<<..<<<<<<.<...<<.....<<<<....>>>>.....>>
.>>>>>..>>>>><<<<...........>>>>........<.........
..<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>.
.>>.>>>>>>>..>>>>>>....>>....<<<<<........>>>>>...
.<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<..<
<<<<.............>>>>>...<<<<<.....<<<<..<<<......
....>>>...............<<<<<<....>>>>>>..>>>>..>>>>
>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>....
.<<...(((>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>
.<<<....>>>.>>)))......<<<<<<<..<<<<.))))))>>>>>>>
>>>>.........>>>>..>>>>>>.......<<.<...<<<<<<...<<
.......>>...>>>>>>....>.>.>.......<<<<<.<<<<<<..<.
<<<<<<<......>>>>>>>...>.<<<.........>>>.....>>>>>
..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>>
>>....<<<<<<<<..<<<<...<<<<<<...............>>>>>>
..<<<<<...<<<<....<<<<<<<<<<]]].>>>>>..>>>>>...>>>
>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>>.
...>>>>>>>>.......<<<<<<.<<<.<<....<<..........<<<
<<<.....>>>>>>......>>.......<<<<<<...<<<<<.<..<<<
......>>>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>
>..<<<<<.<.............>.....<<<<<<<<<.<<<....<<<<
....<<<<<......>>>>>...>>>>...>>>....>>>>.>>>>>..>
>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7n8b CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations.
• Resolution: 3.05 Å
• Binding residue (original residue number in PDB): N3 L4 R5 T6 K8 R9 L10 G18 K19 R20 K21 W23 L24 P26 S37 R38 R42 K46 V55 T56 V57 H58 S59 K60 S61 R62 R64 R74 H75 G77 Y78 G79 K80 R81 K82 G83 T84 R85 E86 A87 R88 L89 P90 Q92 V93 W95 I96 L99 R100 V101 R103 R104 R110 K117 H118 Y120 H121 Y124 K125 K128 G129 N130 H134 R136
• Binding residue (residue number reindexed from 1): N2 L3 R4 T5 K7 R8 L9 G17 K18 R19 K20 W22 L23 P25 S36 R37 R41 K45 V54 T55 V56 H57 S58 K59 S60 R61 R63 R73 H74 G76 Y77 G78 K79 R80 K81 G82 T83 R84 E85 A86 R87 L88 P89 Q91 V92 W94 I95 L98 R99 V100 R102 R103 R109 K116 H117 Y119 H120 Y123 K124 K127 G128 N129 H133 R135

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7n8b, PDBe:7n8b, PDBj:7n8b
• PDBsum: 7n8b
• PubMed: 35489333
• UniProt: P0CX82|RL19A_YEAST Large ribosomal subunit protein eL19A (Gene Name=RPL19A)