Structure of PDB 4v65 Chain AR Binding Site BS01

Receptor Information

>4v65 Chain AR (length=205) Species: 562 (Escherichia coli)

ARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDY
GVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNV
VYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKK
QSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIV
ELYSK

Ligand information

>4v65 Chain A1 (length=1530)

ugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaacac
augcaagucgaacgguaacaggaagaagcuugcuucuuugcugacgagug
gcggacgggugaguaaugucugggaaacugccugauggagggggauaacu
acuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagaggggg
accuucgggccucuugccaucggaugugcccagaugggauuagcuaguag
gugggguaacggcucaccuaggcgacgaucccuagcuggucugagaggau
gaccagccacacuggaacugagacacgguccagacuccuacgggaggcag
caguggggaauauugcacaaugggcgcaagccugaugcagccaugccgcg
uguaugaagaaggccuucggguuguaaaguacuuucagcggggaggaagg
gaguaaaguuaauaccuuugcucauugacguuacccgcagaagaagcacc
ggcuaacuccgugccagcagccgcgguaauacggagggugcaagcguuaa
ucggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaug
ugaaauccccgggcucaaccugggaacugcaucugauacuggcaagcuug
agucucguagagggggguagaauuccagguguagcggugaaaugcguaga
gaucuggaggaauaccgguggcgaaggcggcccccuggacgaagacugac
gcucaggugcgaaagcguggggagcaaacaggauuagauacccugguagu
ccacgccguaaacgaugucgacuuggagguugugcccuugaggcguggcu
uccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaaggu
uaaaacucaaaugaauugacgggggcccgcacaagcgguggagcaugugg
uuuaauucgaugcaacgcgaagaaccuuaccuggucuugacauccacgga
aguuuucagagaugagaaugugccuucgggaaccgugagacaggugcugc
auggcugucgucagcucguguugugaaauguuggguuaagucccgcaacg
agcgcaacccuuauccuuuguugccagcgguccggccgggaacucaaagg
agacugccagugauaaacuggaggaagguggggaugacgucaagucauca
uggcccuuacgaccagggcuacacacgugcuacaauggcgcauacaaaga
gaagcgaccucgcgagagcaagcggaccucauaaagugcgucguaguccg
gauuggagucugcaacucgacuccaugaagucggaaucgcuaguaaucgu
ggaucagaaugccacggugaauacguucccgggccuuguacacaccgccc
gucacaccaugggaguggguugcaaaagaaguagguagcuuaaccuucgg
gagggcgcuuaccacuuugugauucaugacuggggugaagucguaacaag
guaaccguaggggaaccugcgguuggauca

............<<<............<<<<...<<<<<<<......<<<
.<<<...<..........<<<<<<<<<<....>>>>>>>.>>>.......
>.......<<........<<<<<<..<<...<<<<<<<.<.<<.....<<
<<<......>>>>>......>>.>.....<<<....>>>....<<<<<<.
.<<....>>>>>>>>.>>>>>>>..>>.>>>>>>.<<<....<<<...<<
<<<.<........>>>>>>.>>>......>>>..<<<<<<........>>
...>>>>.>>.<<<<.<<.........>>>>>>.<<<<<..>>>>>...>
>>>>>.........<<<..................>>>...>.>>>>>>.
.<<<<.......<<......>>......>>>>...<.<<<<<......<<
<<<<<<.<.......>>>.>>>>.>>.......>>>>>....>..<<<<<
......<<<<......................>>>>>>>>>...>>>>..
.......................<.<...<<<..<<<<<<.<<<<<<<<<
<......<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>....
<<<<<<<<...<<<<<.<.....<<<<<<<....................
.>>>>>>>.............<....>..>.>>>>>..>>>>.>>>>...
>>>...>.>......<<<.<...<<...<<<<.........>>>>...>>
.>>>>.............<<....<<<<<<<<<<<<.....>>>>>>>>>
>>>..................>>........<........<<<....>>>
......>....>>>........<<.<<<<.<<.<<<.....<<<<<.<.<
<.......<........>...........<<<<<<<......<<<<<<<.
.<<<<<<<....>>>>>>>...<<....>>..>>>>>.>>.<<<......
<<<<<<.........<.<.............<<<<......>>>>...>.
>.......<<<..<<<<<<....<...<<<.....>>>.>.....>>>>>
>.....<<<<<.....>>>>>.........>>>.........>>>...>>
>...>>>...>>>>>>>...>>..>.>>>>>......<<<<<<......<
...<<...<<<....>>>...>>.....>......>>>>>>.........
...<<<<<<..........>>>>>>.............>>>.....<<<<
<<.........>>>>>>.......>>...>>>>.>.>.............
.....<<...<....<<<<.<<<.......<<<<<<.<<<...<<....>
>..>>>>>>>>>......>>>.>>>>....>...>>..............
.....<..............>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v65 The Structure of the E. coli Ribosome Before and After Accommodation: Implications for Proofreading
• Resolution: 9.0 Å
• Binding residue (original residue number in PDB): A1 R2 Y3 L4 P6 K7 L8 K9 R12 K21 R25 Q39 D49 Y50 Q53 L54 R61 R62 L67 E68 R69 Q70 R72 N73 R80 R96 T109 E112 R114 Q115 S118 H119 K120 R127 V128 N130 I131 S133 Y134 K147 Q151 R153 E201 S204 K205
• Binding residue (residue number reindexed from 1): A1 R2 Y3 L4 P6 K7 L8 K9 R12 K21 R25 Q39 D49 Y50 Q53 L54 R61 R62 L67 E68 R69 Q70 R72 N73 R80 R96 T109 E112 R114 Q115 S118 H119 K120 R127 V128 N130 I131 S133 Y134 K147 Q151 R153 E201 S204 K205

Gene Ontology

Molecular Function

• GO:0000900 mRNA regulatory element binding translation repressor activity
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0048027 mRNA 5'-UTR binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006353 DNA-templated transcription termination
• GO:0006412 translation
• GO:0006417 regulation of translation
• GO:0031564 transcription antitermination
• GO:0042254 ribosome biogenesis
• GO:0042274 ribosomal small subunit biogenesis
• GO:0045947 negative regulation of translational initiation
• GO:0046677 response to antibiotic
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v65, PDBe:4v65, PDBj:4v65
• PDBsum: 4v65
• UniProt: P0A7V8|RS4_ECOLI Small ribosomal subunit protein uS4 (Gene Name=rpsD)