Structure of PDB 4w2f Chain AM Binding Site BS01

Receptor Information
>4w2f Chain AM (length=123) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
ARIAGVEIPRNKRVDVALTYIYGIGKARAKEALEKTGINPATRVKDLTEA
EVVRLREYVENTWKLEGELRAEVAANIKRLMDIGCYRGLRHRRGLPVRGQ
RTRTNARTRKGPRKTVAGKKKAP
Ligand information
>4w2f Chain AA (length=1497) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uuggagaguuugauccuggcucagggugaacgcuggcggcgugccuaaga
caugcaagucgugcgggccgcggguccguggucagcggcggacgggugag
uaacgcgugggugaccuacccggaagagggggacaacccggggaaacucg
ggcuaaucccccauguggacccgccccuugggguguguccaaagggcuuu
gcccgcuuccggaugggcccgcgucccaucagcuaguuggugggguaaug
gcccaccaaggcgacgacggguagccggucugagaggauggccggccaca
ggggcacugagacacgggccccacuccugggaggcagcaguuaggaaucu
uccgcaaugggcgcaagccugacggagcgacgccgcuuggaggaagaagc
ccuucgggguguaaacuccugaacccgggacgaaacccccgacgagggga
cugacgguaccgggguaauagcgccggccaacuccgugccagcagccgcg
guaauacggagggcgcgagcguuacccggauucacugggcguaaagggcg
uguaggcggccuggggcgucccaugugaaagaccacggcucaaccguggg
ggagcgugggauacgcucaggcuagacggugggagagggugguggaauuc
ccggaguagcggugaaaugcgcagauaccgggaggaacgccgauggcgaa
ggcagccaccugguccacccgugacgcugaggcgcgaaagcguggggagc
aaaccggauuagauacccggguaguccacgcccuaaacgaugcgcgcuag
gucucugggucuccugggggccgaagcuaacgcguuaagcgcgccgccug
gggaguacggccgcaaggcugaaacucaaaggaauugacgggggcccgca
caagcgguggagcaugugguuuaauucgaagcaacgcgaagaaccuuacc
aggccuugacaugcuagggaacccgggugaaagccuggggugccccgcga
ggggagcccuagcacaggugcugcauggccgucgucagcucgugccguga
gguguuggguuaagucccgcaacgagcgcaacccccgccguuaguugcca
gcgguucggccgggcacucuaacgggacugcccgcgaaagcgggaggaag
gaggggacgacgucuggucagcauggcccuuacggccugggcgacacacg
ugcuacaaugcccacuacaaagcgaugccacccggcaacggggagcuaau
cgcaaaaaggugggcccaguucggauuggggucugcaacccgaccccaug
aagccggaaucgcuaguaaucgcggaucagccaugccgcggugaauacgu
ucccgggccuuguacacaccgcccgucacgccaugggagcgggcucuacc
cgaagucgccgggagccuacgggcaggcgccgaggguagggcccgugacu
ggggcgaagucguaacaagguagcuguaccggaaggugcggcuggau
.....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<..<<
<.<<<..<<<..<<.<<<<<<<<..>>>>>>>>.>>>>>......<<...
....<<<<<<<<..<<...<<<<<<<.<<<<<....<<<<<......>>>
>>.....>>>>>...<<<<<.<<<<<....>>>>>.>>>>>..<<<<...
>>>>.>>>>>>>..>>>>>>>>>><<<....<<<..<<<<<<<<......
.>>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.
<<<<<.<.........>>>>>>.<<<<..>>>>...>>>>>>>.......
.<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<<<......<<
<<....>>>>.....>>>>....<<<<<........<<<<.....>>>>.
.........>>>>>......<<<<<(((...<<<<<.....<<.)))>>.
......>>>>>>>>>>..>>>>>>>>>..........<<<((.....<<<
<...<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....>>>>>>.
...>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<....<<<
<<<<.<..<<<......>>>.....>.>>>>>>>...........<<...
.>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<...<<
...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<..<
<<<<<<<<<...>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<
<<......<<<<....>>>>....>>>..)))).]<<<<<.<<<<<<<.<
<.<<<<<<..<<<<<<<<<<......<<........>>..........<<
<<<<<......<<<<<<<<..<<<<<<<....>>>>>>>....<<.....
.>>...>>>>>>>>.<<<.<<<..<<<<<<.......<<<<<<<<<....
>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<<..<<<.
.<<<.....>>>>>>...>>>>>>>.....<<<<<....>>>>>......
..>>>>.........>>>...>>>>>>>>>...>>>>>>>...>>.>>>>
>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..>>....
>>>.....>>>>>>>......<....<<<<<<<........>>>>>>>..
..>.....>>>>>>....<<<<<<<..........>>>>>>>......>>
...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<<<<<<<
<...<.<<<<....<<<....>>>.>>>>.>..>>>>>>>>>>>>>..>>
>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB4w2f Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.
Resolution2.4 Å
Binding residue
(original residue number in PDB)		K13 R14 V17 Y21 Y23 G24 I25 G26 A28 R29 R44 N77 Y87 R88 R91 H92 L96 P97 V98 R99 Q101 R102 T103 R104 T105 N106 A107 R108 T109 K111 R114 K115 T116 V117
Binding residue
(residue number reindexed from 1)		K12 R13 V16 Y20 Y22 G23 I24 G25 A27 R28 R43 N76 Y86 R87 R90 H91 L95 P96 V97 R98 Q100 R101 T102 R103 T104 N105 A106 R107 T108 K110 R113 K114 T115 V116
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0006412 translation
Cellular Component
GO:0005829 cytosol
GO:0005840 ribosome
GO:0015935 small ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:4w2f, PDBe:4w2f, PDBj:4w2f
PDBsum4w2f
PubMed25306919
UniProtP80377|RS13_THET8 Small ribosomal subunit protein uS13 (Gene Name=rpsM)

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