Structure of PDB 3j9w Chain AM Binding Site BS01

Receptor Information

>3j9w Chain AM (length=119) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

ARIAGVDIPRDKRVVISLTYIFGIGRTTAQQVLKEAGVSEDTRVRDLTEE
ELGKIRDIIDKLKVEGDLRREVSLNIKRLIEIGSYRGIRHRRGLPVRGQN
SKNNARTRKGPRRTVANKK

Ligand information

>3j9w Chain AA (length=1544)

ggagaguuugauccuggcucaggacgaacgcuggcggcgugccuaauaca
ugcaagucgagcggacagaugggagcuugcucccugauguuagcggcgga
cgggugaguaacacguggguaaccugccuguaagacugggauaacuccgg
gaaaccggggcuaauaccggaugguuguuugaaccgcaugguucaaacau
aaaagguggcuucggcuaccacuuacagauggacccgcggcgcauuagcu
aguuggugagguaacggcucaccaaggcgacgaugcguagccgaccugag
agggugaucggccacacugggacugagacacggcccagacuccuacggga
ggcagcaguagggaaucuuccgcaauggacgaaagucugacggagcaacg
ccgcgugagugaugaagguuuucggaucguaaagcucuguuguuagggaa
gaacaagugccguucgaauagggcgguaccuugacgguaccuaaccagaa
agccacggcuaacuacgugccagcagccgcgguaauacguagguggcaag
cguuguccggaauuauugggcguaaagggcucgcaggcgguuucuuaagu
cugaugugaaagcccccggcucaaccggggagggucauuggaaacugggg
aacuugagugcagaagaggagaguggaauuccacguguagcggugaaaug
cguagagauguggaggaacaccaguggcgaaggcgacucucuggucugua
acugacgcugaggagcgaaagcguggggagcgaacaggauuagauacccu
gguaguccacgccguaaacgaugagugcuaaguguuaggggguuuccgcc
ccuuagugcugcagcuaacgcauuaagcacuccgccuggggaguacgguc
gcaagacugaaacucaaaggaauugacgggggcccgcacaagcgguggag
caugugguuuaauucgaagcaacgcgaagaaccuuaccaggucuugacau
ccucugacaauccuagagauaggacguccccuucgggggcagagugacag
guggugcaugguugucgucagcucgugucgugagauguuggguuaagucc
cgcaacgagcgcaacccuugaucuuaguugccagcauucaguugggcacu
cuaaggugacugccggugacaaaccggaggaagguggggaugacgucaaa
ucaucaugccccuuaugaccugggcuacacacgugcuacaauggacagaa
caaagggcagcgaaaccgcgagguuaagccaaucccacaaaucuguucuc
aguucggaucgcagucugcaacucgacugcgugaagcuggaaucgcuagu
aaucgcggaucagcaugccgcggugaauacguucccgggccuuguacaca
ccgcccgucacaccacgagaguuuguaacacccgaagucggugagguaac
cuuuuaggagccagccgccgaaggugggacagaugauuggggugaagucg
uaacaagguagccguaucggaaggugcggcuggaucaccuccuu

...<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<.
<<<..<<<..<<.<<<<...<<<<<....>>>>>...>>>>.>>>>>...
...<<........<<<<<<<..<<...<<<<<<<.<<<<.....<<<<<<
....>>>>>>......>>>>.....<<<<<<<<<<....>>>>>>>>>>.
....<<<<<<....>>>>>>.>>>>>>>..>>>>>>>>>.<<<....<<<
..<<<<<<<<.......>>>>>>>>>>>......>>>..<<<<<<<<...
.>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>
>...>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>
>>>>..<<<<......<<<<....>>>>.....>>>>..<<<<<<<<...
....<<<<<<<<<<<.....>>>>>>>>>.>>.......>>>>>>...>>
.<<<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>
>>>>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<
<<<<<<.......<<<<<<.....>>>>>>.....>>>>>>>..>>>>>>
>>>...<<<.<<<<...<<<<<<<....<<<<<<<<...<<<......>>
>......>>>>>>>>...........<<....>>.>>>>>>>..>>>>..
>>>...>>>...>>>>....<<<<<<...<<...<<<<.........>>>
>...>>>>>>>>..........<<<<<<..<<<<<<<<<<<<<....>>>
>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<......<<<<
....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<
<<<<<<........<<........>>..........<<<<<<<......<
<<<<<<....<<<<<....>>>>>....<<<....>>>.>>>>>.>>.<<
<.<<<..<<<<<<.......<<<<<<<<<....>>>..<<<<......>>
>>..>>>>>>.....<<<<.<<<<<<<..<<<..<<......>>>>>...
>>>>>>>.....<<<<<.....>>>>>........>>>>.........>>
>...>>>>>>>>>...>>>>>>>......>>>>>>>>.....<<<<<<<.
....<<<..<<..<<<<....>>>>..>>....>>>.....>>>>>>>..
....<....<<<<<<<........>>>>>>>....>.....>>>>>>...
..<<<<<<.........>>>>>>.......>>...>>>>>>>>>>.>>..
..<..<<.<.<<<<.<<<..<<<<<<..<<<<....<.<<<<..<<<..<
<<...>>>.>>>.>>>>.>...>>>>..>>>>>>..>>>.>>>>..>.>>
...>.....<<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j9w Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): G24 G26 P96 V97 N101 S102 K103 A106 R107 R114
• Binding residue (residue number reindexed from 1): G23 G25 P95 V96 N100 S101 K102 A105 R106 R113

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j9w, PDBe:3j9w, PDBj:3j9w
• PDBsum: 3j9w
• PubMed: 25903689
• UniProt: P20282|RS13_BACSU Small ribosomal subunit protein uS13 (Gene Name=rpsM)