Structure of PDB 8hl1 Chain AL6P Binding Site BS01

Receptor Information

>8hl1 Chain AL6P (length=181) Species: 330779 (Sulfolobus acidocaldarius DSM 639)

LKAAYIREEIQIPDKVKVSLENNVLKVKGPKGEVIKDFSYAKGIRIQLNE
GKIILETTFADRRKKALLYSIIAHIKNMITGTINGYRYYLKVISTHFPIS
VKVSGDEVQVSNLIGEKNIRRAKILPGVKVTVKGEDIVVEGSDIYNVAQT
AANIESSTKIVGYDRRIFSDGIYIYKKEVIG

Ligand information

>8hl1 Chain A23S (length=2995)

aagccucccgguggauggcucggcucgggcaccgaagaagggcgcggcaa
gcagcgaaaugcucgggugaggcgcaagcagccguugaccccgagguccc
cuaaugggauauccugccggguuuccggcucccgguuuauaacugggugg
gaaccccccgaacggaaacaucuuaguagggggaggaaaagaaaucaauu
gagauccccugaguaggggcgaccgaaagggggacagcccaaacuaaacc
ugccgaugauaagucgguggggaugugguguuacgaccucuagccugagg
uugaccucggcuuuccuaaccuaucuagccgaacuccccuggaacggggg
gccauagagggugaaagccccguaggcuaaagauagguggaaaguggcua
gagguagaguaccauccccugguuugggggugggaaguuaggggacacgu
gccucuaaggcuaaauaugucccgagaccgauagcaaacuaaguaccgug
agggaaagcugaaaagaaccccggaagggggagugcaaagagccugaaac
cgggugguuauacaggguguggcucgaaagaagugaacccuuccgaagga
aaggggcgcaagcccuuaguacgaggaagggcgaucggggucacgccuuu
cgucuugaaacacgggccggggaguucacaucaguggcgagcuuaaggag
auuaucuccgaaggcauagggaaaccaagugcccgcagccuaguuucuag
gcgaggggcagggucugucagggccugaagccacugaugugaggcuagaa
accgggcgaucuaguccggggcaggcugaaggugggggaaaccccacugg
agggccgaauagggguucugacgugcaauucguucccuugaccucggacu
aggggcaaaagaccaaucuagcccggugauagcuaguuccccccgaaaug
cguccuagcgcagccucccuaaaggcagcucgcgggguagagugacagau
cgggggcuccaggcgccuggggcuuccggucuaacuccgaacccacgagc
gccgaagaaggggggagugggucacucggcguaagguuggguggcaaaag
gggaacaacccagaccuggguuaaggccccaaagucccggcuaagugcca
acgaaaaggcgucuccagccuuagacagcgggaaggugggcccagcagca
gccauccucuaaggagugcguaacagcucacccgccgaggcuggaggccc
uaaagauuggucggggcucaagccgggcgccgagacccaggagggggucu
acuagagauccucugguaggggggcgcugugaugggguagaagguggguc
gugagauccacuggacccgucacaggugcagaucccggcgguaguaacag
cgaaggggggugagaaucccccucgccggaagggcaaggguuucccggca
acguucgucagccgggaguuagccgccuaagguagggccuaauagguacc
uaccgaaagggaaagggguuaacauuccccugccucccggguaggugcgg
uaacgcaagccagacuccugacggauugggguagggagaguaggaccacc
guccuacccaagcacucaagcccuuggagagccguaacggugagaagagg
gcgaaggugugaugggccuuccguuaggaggguucuccugaucccuaguc
cccaugaaaagggagucuggaacgaucccgggagaccguaccuagaaccg
acacuggugcccgggugagaagcccaaggcgucugagggguaacccaggc
uagggaacucggcaaauuagccccguaacuucgggagaaggggugccuau
cgugguuuaacaaagccacgauaggucgcagugaccagagggaccugacu
guuuaauaaaaacauagguccccgcuagcccgaaaggguguguacggggg
cuaaauccuggccacuggugguugaaauccggguucaaccgggcgaagcc
ccaccgaaggccggggguaacucugacccucuuaagguagccaaaugccu
ugccggguaaguuccggcgcgcaugaauggaucaacgcggucccuacugu
cccagccuggggccucgugaacgcccugagccggugcacaguccggcauc
ucccuacaccgagagaagaccccguggagcuucaccgcagccuggcguug
ucccucgggcguuuaugcguagaguaggugggaggggucgaaccuguccu
uucgggggcaggggacccgaaagugaaacaccacccauggacgcucgagg
gacuaaccucucgaaagagaggaacaacgucaggugggcgguucggcugg
ggcggcacucccgcgaaaagauaacacgggagcccaaaggucggcucagg
cgguacagaacgccgccguagagcgcaagggcaaaagccggccugacgug
acccuuccaaguacggggucacgacgcgaaagcggggccuagcgaacgcu
cgugcccccacacgugggggccgggcaugacagaaaaguuaccccgggga
uaacagggucgucgcgggcgagagcucacaucgaccccgcgguuugcuac
aucgaugucggcucuucccacccuggaggugcagcugccuccaaggguag
ggcugcccgcccguuaaaggggagcgugagcuggguuuagaccgucgcga
gacaggucggacucuaaggguagggagugcggaccgcuugaggggaagga
accccuaguacgagaggaacaggguuccggggccuccaguuuaccgguug
uccgguagggcaaugccgggcagccgcgcccugagggguaaccgcugaaa
gcaucuaagcgggaaccccuccccuaaaagaggcggucaggcgccggggc
cuuccccuagaagaggggguugaugggguggggauguaagcuccaagguu
guaagaccgaggaguuuaguccgccacucccaaucaggcuccccc

<.<<<.<<<<<<....<<<..<<<<<<<<<<<<.....<<<<<<<.((..
((.>>>.....<<<<<<...<<<))..)).>>>......>>>>>>...>>
>>...<<<....>>><<<<<<..>>>>>>.<<<<<<.....>>>>>>.>>
...<<<<<<....<<......>>.....>>>>>.>.........<<<..>
>>..<<<<<<.....<<.....>>....>>>>>>..<<<...<<<...<<
<<<<<<<.....>>>>>>>>>.....>>>......<<<<<<<<<<<<<<<
<..>>>>>><<<<<<<..<<<<<<<<.<<<.....<<<<......>>>>.
((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>>>>>
>>>>>......<<<<<<<<......>>>>>>>>......<<<<<.(((((
(>>>>>..>>>......>>.>>>>>>.>>................<<...
.>>....>>.........<<<<.....>>>>............>....>>
>>>>.>>>>......<<<<<<<<<<....<<......<<<<<<<...<..
..<<<<<....>>>>>.....>..>>>>>>>...>>.>>>>>>>>>>..<
<<<........>>>><<<<<<...<<<<<<<<<<<<<<..<<<...<<<<
<...>>>>>...>>>...<<....>>...<<<<<...<<<<.......>>
>>...>>>>><<<<<<....>>>>>>...>>>>>>>>>>>>>>.<<<<..
.<<<<<<.....<<<<<<<<<..<<<<...<<<<<<<....>>>>>>>..
.>.>>>....<<<<<....<<........>>..>>>>>...>>>>>>>>>
...<<.......>>......>>>>>>......>>>>..<<<<<<....<<
<<<....>>>>><<<<<<<<...<<<.<<<<<.<<<...<<<....<<<<
<<<<<<<<<<<<<..>>>>>>>>>>>>>>>>>..>>.>...>>>.>>>>>
>>>......>>>>>>.>>..<<<<<<.<<<.....>>>.>>>>>>....<
<<......>>>...<<<<<<<...<<<(((.....<<<<<<.....<<..
......<<<.<<<<<<<<<<.......<<<<<.<<.....<.........
>.....>>.........<<......>>...>>>>>...>>>>>>>>>>>>
>...........)))>>....>>>>>>.>>>...>>>>>>><<<<<<<.<
....>.>.>>>>>>....>>>>>><<<<<<<<<.<<<.....<<<<<<<<
....>>>>>>>>...>>>>>>>>>>.>>.....>>>>>><......<<..
....<<<<<<.......>>>>>><<<<....<<<<<<<<...<<<<<<..
..<.....>..>>>>>>....<<.{<<...<<<<<<<<<.....>>>.>>
>>>>....>>...<<<<<........>>>>>>><<<<<<<<.....<<<.
...>>>...<<<<<<<<<<...<<<<<<<<<..<<<<<<<<<<<<<.[[[
>>>>>>><<..<<<<<...<<<<<<.....<<<<...>>>>.....>>>>
>>...>>>>>...>><<<<<<<....>>>>>>>>>>>>>...>>>>>>>>
>.........>>>>>>>>>>......>>>>>>>>.}....>>>....<<.
.....>>>>>>><<<<.....>>>>..>>>>...<<<<.....<<<<<<<
<.<<...............<<<<<....<....>.....>>>>><<<<<<
<<<<<<<......>>>>>>>>>>>>>............<<<<<<<<<..<
<<.........>>>..<<<<<<<....<<<<....>>>>......>>>>>
>>..<<<<.<<<..<<<<<<<......<<<<<......>>>>>.......
>>>>>..>>>>>.<<<<<.......>>>>>.........<..........
.<<<<<<.....>>>>>>.....>....>>>>...>>.>>>>>>>.....
>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<
<<<<<<<.<<..<<......<<<<.<<<<<<..<<<<<.<<<<<<<<<.<
<<<<<<<<<<<<<<<<..........<<<<<..<<<......<.<<.<<<
.....>>>>>.>...>>>............>>>>>.>>>>>>>>>>>>>>
>>>...<<<<<<....>>>>>>....>>>>>>>>>..>>>>>...<<<..
...>>>..<<<<<.............>>>>>..<<...<<<<<<<...<<
<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<
<<<<<.........>>>>>>>>>.<<<....>>>.>>))).......<<<
<<.<<<<<..))))))>>>>>>>>>>..........>>>>..>>>>>>..
.....<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>
.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>..<
<<<.....>>>>......>>>>>..>...>>>.>>....<<<<<<<....
>>>.>>>>....>>...>>>>>>>>>>....<<<<<<<..<<<<<..<<<
<<<<.................>>>>>>><<<<<...<.<<...<<<<<<<
<<<]]].>>>>>..>>>>>...>>.>.>>>>>.<<<<<...<<<<<....
.......>>>>>...>>>>>>>>>>......>>>>>>>.<<..>><<<<<
<<<<<<<.......>>>>>....<<<<<<<<<<<<....<<<<<..<<<<
....>>>>..>>>>>...>>>>.>>>>>.>>>...>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8hl1 Cryo-electron microscopy structure and translocation mechanism of the crenarchaeal ribosome.
• Resolution: 3.93 Å
• Binding residue (original residue number in PDB): L4 R65 R66 K68 A69 L70 Y72 S73 H77 N80 F100 G118 E119 K120 N121 Y148 A151 Q152 K162 D167
• Binding residue (residue number reindexed from 1): L1 R62 R63 K65 A66 L67 Y69 S70 H74 N77 F97 G115 E116 K117 N118 Y145 A148 Q149 K159 D164

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8hl1, PDBe:8hl1, PDBj:8hl1
• PDBsum: 8hl1
• PubMed: 37604686
• UniProt: O05637|RL6_SULAC Large ribosomal subunit protein uL6 (Gene Name=rpl6)