Structure of PDB 7toq Chain AL34 Binding Site BS01

Receptor Information

>7toq Chain AL34 (length=114) Species: 9986 (Oryctolagus cuniculus)

VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPG
RLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQK
IVVKVLKAQAQSQK

Ligand information

>7toq Chain A25S (length=3649)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcgcgguccggccgugucggcg
gcccggcggaucucccgcucccgucggcgggggaccgucccccgaccggc
gaccggccgccgccgggcgcauuuccaccgcggcggugcgccgcgaccgg
cuccgggacggcugggaaggccccggcggggaagguggcccgggggcccc
ggguguuacagccccgccccggcagcagcagcucgccgaaucccggggcc
gagggagcgagacccgugcgccgcgcucuccccccucgggggggccgggc
caccccucccacggcgcgaccgcuggggcggacuguccagugcgccccgg
gcgggucgcgccgucgggcccggggggccacgcgucccccgcgagcgcac
ggggucggcggcgaugucggcuacccacccgacccgucuugaaacacgga
ccaaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccg
uggcgcaaugaaggugaaggccggcgcgcucgccggccgaggugggaucc
cgaggcaguccgccgagggcgcaccaccggcccgucucgcccgccgcgcc
ggggagguggagcacgagcgcacguguuaggacccgaaagauggugaacu
augccugggcagggcgaagccagaggaaacucugguggagguccguagcg
guccugacgugcaaaucggucguccgaccuggguauaggggcgaaagacu
aaucgaaccaucuaguagcugguucccuccgaaguuucccucaggauagc
uggcgcucuccgcaguuuuauccgguaaagcgaaugauuagaggucuugg
ggccgaaacgaucucaaccuauucucaaacuuuaaauggguaagaagccc
ggcucgcugguguggagccgggcguggaaugcgagugccuagugggccac
uuuugguaagcagaacuggcgcugcgggaugaaccgaacgccggguuaag
gcgcccgaugccgacgcucaucagaccccagaaaagguguugguugauau
agacagcaggaugguggccauggaagucggaauccgcuaaggagugugua
acaacucaccugccgaaucaacuagcccugaaaauggauggcgcuggagc
gucgggcccauacccggccgucgccggcagucggcagagcgagaaagaga
guggcgcggacgcuacgccgcgacgaguaggagggccgcugcggugagcc
uugaagccuagggcgcgggcccggguggagccgccgcaggugcagaucuu
ggugguaguagcaaauauucaaacgagaacuuugaaggccgaaguggaga
aggguuccaugugaacagcaguugaacaugggucagucgguccugagaga
ugggcgagcgccguuccgaagggacgggcgauggccuccguugcccucgg
ccgaucgaaagggagucggguucagauccccgaauccggaguggcggaga
ugggcgccgcgaggcguccagugcgguaacgcgaccgaucccggagaagc
cggcgggagccccggggagaguucucuuuucuuugugaagggcagggcgc
ccuggaauggguucgccccgagagaggggcccgugccuuggaaagcgucg
cgguuccggcggcguccggugagcucucgcuggcccuugaaaauccgggg
gagaggguguaaaucucgcgccgggccguacccauauccgcagcaggucu
ccaaggugaacagccucuggcauguuggaacaauguagguaagggaaguc
ggcaagccggauccguaacuucgggauaaggauuggcucuaagggcuggg
ucggucgcggccggcgccuagcagccgacuuagaacuggugcggaccagg
ggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcggcggg
uguugacgcgaugugauuucugcccagugcucugaaugucaaagugaaga
aauucaaugaagcgcggguaaacggcgggaguaacuaugacucucuuaag
guagccaaaugccucgucaucuaauuagugacgcgcaugaauggaugaac
gagauucccacugucccuaccuacuauccagcgaaaccacagccaaggga
acgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacuc
uagucuggcacggugaagagacaugagagguguagaauaagugggaggcc
ggccgccggugaaauaccacuacucugaucguuuuuucacugacccggug
aggcgggggggcgagccccgaggggcucucgcuucuggcgccaagcgccc
ggccgcgcgccggccgggcgcgacccgcuccggggacagugccagguggg
gaguuugacuggggcgguacaccugucaaacgguaacgcagguguccuaa
ggcgagcucagggaggacagaaaccucccguggagcagaagggcaaaagc
ucgcuugaucuugauuuucaguacgaauacagaccgugaaagcggggccu
cacgauccuucugaccuuuuggguuuuaagcaggaggugucagaaaaguu
accacagggauaacuggcuuguggcggccaagcguucauagcgacgucgc
uuuuugauccuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauacggca
gcgccggagccucgguuggccucggauagccggucccccgcgggguccgg
ugcggagagcccuucgucccgggaaacggggcggccggaaagggggccgc
ccucucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaacc
auucguagacgaccugcuucugggucgggguuucguacguagcagagcag
cucccucgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<<....<......>.....>
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>>>>>>..[[[...<<<<...<<<<<<<<<<<...>>>>>>>.>>>>...
..>>>>...<<<<<<<<<....<<<<<<<<.<......<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<.<<<.....<<<<<<<<<<....<<<<<<<.<<<<<.<
<....<<<<.<<<<<<<<<......>>>>>>>>>>>>>>>>>.>>>>>>>
...>>>.>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<<<<<
<<<<<<<<<<<<...<<..<<<..<<<<<<<......<<<<<<<<.>>>>
>>>>.......>>>>>>>..>>>.........>>>>>>..>>>>>>>>>>
>.....<<.......>>.........<...<<<<<<..>>>>>>....<<
<...<<.<<<<<<<<<<<<<<<<<<<<<<<.<<<<..>>>>.>>>>>>.>
>>.>>>>>>>>>>>.>>>...>>.>>.>................>.....
...>>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>><
<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>....
.>>>...<<....>>...<<<<<<<<....>>>>>>>>..<<<<<...<<
<..<<.{.[[..>>..>>>....>>>>>.<<<<<<<<<.<<<<......>
>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<...<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
..<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>
......>>>>>>......>.>>..<<<<<<....<.<<<....>>>.><<
<<<<<<<<...<<<.<<<<<<<<<...<<<....<<<..<.<<<..<<<<
.<.<.....>.>.>>>>>>>.>..>>>..>.>>...>>>>>>>...<<<<
<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<<<<.
<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<
<<(((.......<<<<<.....<<..........<<<.<<<<<<<<<<..
.....<<<<<.<<.....................>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>
>>>..>>>...>>>>>>><<<<<<.<<<....................]]
.}..............>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<.
.....<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>
>>><<<....<<.....<<<<<<.......>>>>>>.<<<.....<<<<<
.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<..
..<<<....>>>..........<<.<<<<<<<<<...>>>>>>>>>>>..
....>>....>>...<<<<<........>>>>>))<<<<....<<<<...
<<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<
<<<<<<<<<<<<<<<<<<<<<..{{{..>>>>>>.....<<<<.<<<<.<
<<<.....<<<.....>>>.....>>>>>>>>..>>>>......<<<<<<
<.......>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>>>>>>
..>..........>..>>>.>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<......
.........<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<..
.<<<<<..>>>>>........>>>>>..>>>>>>.>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>
>......>>>>>>>...<<..<<<..<<<<<<....<<<.....<<<<..
..>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<.......>>>>>.....>.....>>....>
>.>>>>>>>.....>>.>>>>>>>..>>>>>>...>>>....<<<<<...
.....>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<
<.<<<<<<<<<.<<<<<<<<<<<<.<<<<..........<<<<<..<<<.
.>>>............>>>>>.>>>>.>>.>>>>>>>>>>...<<<<<..
...<<<<..<<.<.<<<<....>>>>.>>>..............<<<<<<
<<<<<.....>>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>
>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<<...
<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>
>>>>>...<<<..<<<<.......>>>>..>>>.<<<....>>>.>>)))
.......((((((..(((..>>>>>>.)))))))))..........]]]]
..}}]]]].......<<.<...<<<<<<...<<.......>>...>>>>>
>....>.>.>.........<<<.<<<<<<..<.<.<<<<<......>>>>
>...>.>.<<<.<.....>.>>>.....>>>>>..>...>>>.......<
<<<<<<....>>>.>>>>....}}..}}}.]]]]].....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<<}}}.>>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>......
<<<<<<...<<<<....<<<........<<<<<..<<<<..>>>>.>>>>
>.......>>>...<<<..<<<<....<<<<<<<<<........>>>>>>
.>>>...>>>>..>>>..............>>>>....>>>>>>.<<<<<
.<.............>....<<.<<<<<<<.<<.....<<<<....<<<<
<......>>>>>...>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7toq Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): V2 R4 T6 Y7 R8 R9 R10 S12 Y13 N14 T15 S17 R21 R24 T25 P26 G27 N28 R29 Y32 Y34 K36 K37 K40 P42 K43 V48 P50 G51 R54 G55 R57 R60 P61 K62 L64 M65 R66 S68 K69 T70 K72 H73 V74 S75 R76 Y78 G79 G80 S81 A84 R90 R93 A94 I97 Q100 Q112 K115
• Binding residue (residue number reindexed from 1): V1 R3 T5 Y6 R7 R8 R9 S11 Y12 N13 T14 S16 R20 R23 T24 P25 G26 N27 R28 Y31 Y33 K35 K36 K39 P41 K42 V47 P49 G50 R53 G54 R56 R59 P60 K61 L63 M64 R65 S67 K68 T69 K71 H72 V73 S74 R75 Y77 G78 G79 S80 A83 R89 R92 A93 I96 Q99 Q111 K114

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7toq, PDBe:7toq, PDBj:7toq
• PDBsum: 7toq
• PubMed: 35589706
• UniProt: G1TXG5|RL34_RABIT Large ribosomal subunit protein eL34 (Gene Name=RPL34)