Structure of PDB 7top Chain AL34 Binding Site BS01

Receptor Information

>7top Chain AL34 (length=112) Species: 4932 (Saccharomyces cerevisiae)

AQRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSAL
QGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIV
KKVVKEQTEAAK

Ligand information

>7top Chain A25S (length=3194)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaggccagcaucag
uuuugguggcaggauaaauccauaggaauguagcuugccucgguaaguau
uauagccugugggaauacugccagcugggacugaggacugcgacguaagu
caaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacc
aaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcgua
augaaagugaacguagguuggggccucgcaagaggugcacaaucgaccga
uccugaugucuucggauggauuugaguaagagcauagcuguugggacccg
aaagauggugaacuaugccugaauagggugaagccagaggaaacucuggu
ggaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguau
aggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguu
ucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaaug
auuagagguuccggggucuugaccuauucucaaacuuuaaauauguaaga
aguccuuguuacuuaauugaacguggacauuugaaugaagagcuuuuagu
gggccauuuuugguaagcagaacuggcgaugcgggaugaaccgaacguag
aguuaaggugccggaauacacgcucaucagacaccacaaaagguguuagu
ucaucuagacagccggacgguggccauggaagucggaauccgcuaaggag
uguguaacaacucaccggccgaaugaacuagcccugaaaauggauggcgc
ucaagcguguuaccuauacucuaccgucaggguugauaugaugcccugac
gaguaggcaggcguggaggucagugacgaagccuagaccguaaggucggg
ucgaacggccucuagugcagaucuuggugguaguagcaaauauucaaaug
agaacuuugaagacugaaguggggaaagguuccacgucaacagcaguugg
acguggguuagucgauccuaagagauggggaagcuccguuucaaaggccu
gauuuuaugcaggccaccaucgaaagggaauccgguuaagauuccggaac
cuggauauggauucuucacgguaacguaacugaauguggagacgucggcg
cgagcccugggaggaguuaucuuuucuucuuaacagcuuaucaccccgga
auugguuuauccggagauggggucuuauggcuggaagaggccagcaccuu
ugcuggcuccggugcgcuugugacggcccgugaaaauccacaggaaggaa
uaguuuucaugccaggucguacugauaaccgcagcaggucuccaagguga
acagccucuaguugauagaauaauguagauaagggaagucggcaaaauag
auccguaacuucgggauaaggauuggcucuaagggucgguacgaucaacu
uagaacugguacggacaaggggaaucugacugucuaauuaaaacauagca
uugcgauggucagaaagugauguugacgcaaugugauuucugcccagugc
ucugaaugucaaagugaagaaauucaaccaagcgcggguaaacggcggga
guaacuaugacucucuuaagguagccaaaugccucgucaucuaauuagug
acgcgcaugaauggauuaacgagauucccacugucccuaucuacuaucua
gcgaaaccacagccaagggaacgggcuuggcagaaucagcggggaaagaa
gacccuguugagcuugacucuaguuugacauugugaagagacauagaggg
uguagaauaagugggagcuucggcgccagugaaauaccacuaccuuuaua
guuucuuuacuuauucaaugaagcggagcuggaauucauuuuccacguuc
uagcauucaaggucccauucggggcugauccggguugaagacauugucag
guggggaguuuggcuggggcggcacaucuguuaaacgauaacgcagaugu
ccuaaggggggcucauggagaacagaaaucuccaguagaacaaaagggua
aaagcccccuugauuuugauuuucagugugaauacaaaccaugaaagugu
ggccuaucgauccuuuagucccucggaauuugaggcuagaggugccagaa
aaguuaccacagggauaacuggcuuguggcagucaagcguucauagcgac
auugcuuuuugauucuucgaugucggcucuuccuaucauaccgaagcaga
auucgguaagcguuggauuguucacccacuaauagggaacgugagcuggg
uuuagaccgucgugagacagguuaguuuuacccuacugaugaauguuacc
gcaauaguaauugaacuuaguacgagaggaacaguucauucggauaauug
guuuuugcggcugucugaucaggcauugccgcgaagcuaccauccgcugg
auuauggcugaacgccucuaagucagaauccaugcuagaacgcggugauu
ucuuugcuccacacaauauagauggauacgaauaaggcguccuuguggcg
ucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccuug
ggugcuugcuggcgaauugcaaugucauuuugcguggggauaaaucauuu
guauacgacuuagauguacaacgggguauuguaagcaguagaguagccuu
guuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...<.......<<.<<<<<<...>>>>>>.>>.......>
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<<.....<<<<<<<.<<<
<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<<..>>>>>>>..
.....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>
>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><<<
<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...
<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<
<<<...<......>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<
..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>
.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>
...<<.......>>......>>>>>>...>..>.>>..<<<<<<....<.
<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<....<<
<....<<<<...<<....>>.>>>>....>>>..>.>>.....>>>>>..
.<<<<.<<<<.<......>>>>>.>>>>.....>>>>>.>>>>>>>>.>>
..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<
<<<..<<<<(((.....<<<<<<.....<<..........<<<.<<<<<<
<<<<.......<<<<<.<<....<<.........>>....>>........
.<<......>>...>>>>>...>>>>>>>>>>>>>..........))).>
>...>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>>>
>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>>>>>>
>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<..
.....>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>>.
>>>>>>....((<<<<<<...<<....<<<....>>>........<<<.<
<........>>.>>>....>>....>>...<<<<<........>>>>>))
<<<<.<<<<<<<.....<<<....>>>.......<<<<<<...<<<<.<<
<<<<<.<<<<<<<<<<..[[[..>>>>>><...<<<<<....<<<<<<..
..<<<.....>>>....>>>>>>.....>>>>>....><<<<<<<<....
>>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>........
..>>>..>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<..
...>>>>..>>>>..<<<<.....<<<<<<<.<<...............<
<<<<....<<..>>.....>>>>><<.<<<<<<<<.........>>..>>
>>>>.>>............<<<<<<<<<..<<<.........>>>..<<<
<<<<....<<<<.....>>>>......>>>>>>>.<.<<..<<<..<<<<
<<.<...<<....<<<<<....>>>>.>...>>.>>>>>..>>>>><<<<
<<.......>>>>>>........<...........<<<<<.......>>>
>>.....>.....>>.>..>>.>>>>>>>.....>>.>>>>>>>..>>>>
>>....>>....<<<<<........>>>>>....<<<<<..<<<.<<...
...<<<<.<<<<<<..<<<<<.<<<<<<<<..<<<<<<<<<<<<..<<..
...........<<<......................>>>....>>..>>.
>>>>>>>>>>...<<<<<.....<<<<..<<<<........>>>>.....
..........<<<<<<....>>>>>>..>>>>..>>>>>.....>>>>>>
>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..
<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>.
...>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.
>>)))......<<<<<<<..<<<<.))))))>>>>>>>>>>>........
.>>>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>>...
>>>>>>...>>..>>.......<<<<<.<<<<<<..<.<<<<<<<.....
.>>>>>>>...>.<<<.........>>>.....>>>>>..>...>>>.>>
....<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<<<<<
<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<...<<
<<....<<<<<<<<<<]]].>>>>>..>>>>>...>>>>..>>>>>.<<<
<...<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>>.
......<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>
>>......>>.......<<<<<<...<<<<<.<..<<<......>>>..>
.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<..
...........>.....<<<<<<<<<.<<<....<<<<....<<<<<...
...>>>>>...>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7top Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): A2 R4 V5 T6 F7 R8 R9 R10 N11 P12 Y13 N14 T15 R16 S17 V23 K24 T25 P26 G27 L30 R31 K36 K37 L38 T40 R41 P42 K43 G45 Q52 G53 I54 S55 T56 R58 P59 R60 Y62 A63 T64 S66 K67 K70 T71 S73 R74 Y76 G78 N83 R88 R91 A92 I95 E96
• Binding residue (residue number reindexed from 1): A1 R3 V4 T5 F6 R7 R8 R9 N10 P11 Y12 N13 T14 R15 S16 V22 K23 T24 P25 G26 L29 R30 K35 K36 L37 T39 R40 P41 K42 G44 Q51 G52 I53 S54 T55 R57 P58 R59 Y61 A62 T63 S65 K66 K69 T70 S72 R73 Y75 G77 N82 R87 R90 A91 I94 E95

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7top, PDBe:7top, PDBj:7top
• PDBsum: 7top
• PubMed: 35589706
• UniProt: P87262|RL34A_YEAST Large ribosomal subunit protein eL34A (Gene Name=RPL34A)