Structure of PDB 7tor Chain AL21 Binding Site BS01

Receptor Information

>7tor Chain AL21 (length=159) Species: 9986 (Oryctolagus cuniculus)

TNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKG
MPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKS
RDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELL
EPIPYEFMA

Ligand information

>7tor Chain A28S (length=3552)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgcgggggcccaaguccuucuga
ucgaggcccagcccguggacggugugaggccgguagcggcccccggcgcg
ccgggcccgggucuucccggagucggguugcuugggaaugcagcccaaag
cgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauaguc
aacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaa
gagggcgugaaaccguuaagagguaaacgggugggguccgcgcaguccgc
ccggaggauucaacccggcggcgcgguccggccgugccggcggggcggau
ccccgcucucggcgggggaccgccccccggccggcgaccggccgccgccg
ggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacggcugg
gaaggccccggcggggaagguggcccgggggccccggguguuacagcccc
gccccggcagcagcagcucgccgaaucccggggccgagggagcgagaccc
gugcgccgcgcucuccccccggggggccgggccaccccucccacggcgcg
accgcuggggcggacuguccagugcgccccgggcgggucgcgccgucggg
cccggggggccacgcguccagcgcacggggucggcggcgaugucggcuac
ccacccgacccgucuugaaacacggaccaaggagucuaacacgugcgcga
gucgggggcuacgaaagccgccguggcgcaaugaaggugaaggccggccg
gccgaggugggaucccgaggcaguccgccgagggcgcaccaccggcccgu
cucgcccgccgcgccggggagguggagcacgagcgcacguguuaggaccc
gaaagauggugaacuaugccugggcagggcgaagccagaggaaacucugg
uggagguccguagcgguccugacgugcgaaucggucguccgaccugggua
uaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccaaagu
uucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagcgaau
gauuagaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaa
auggguaagaagcccggcucgcugguguggagccgggcguggaaugcgag
ugccuagugggccacuuuugguaagcagaacuggcgcugcgggaugaacc
gaacgccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaa
gguguugguugauauagacagcaggaugguggccauggaagucggaaucc
gcuaaggaguguguaacaacucaccugccgaaucaacuagcccugaaaau
ggauggcgcuggagcgucgggcccauacccggccgucgcuggcagucggc
agagcgagaaagagagggacgcuacgccgcgacgaguaggagggccgcug
cggugagccuugaagccuagggcgcgggcccggguggagccgccgcaggu
gcagaucuuggugguaguagcaaauauucaaacgagaacuuugaaggccg
aaguggagaaggguuccaugugaacagcaguugaacaugggucagucggu
ccugagagaugggcgagcgccguuccgaagggacgggcgauggccuccgu
ugcccucagccgaucgaaagggagucggguucagauccccgaauccggag
uggcggagaugggcgccgcgaggcguccagugcgguaacgcgaccgaucc
cggagaagccggcgggagccccggggagaguucucuuuucuuugugaagg
gcagggcgcccuggaauggguucgccccgagagaggggcccgugccuugg
aaagcgucgcgguuccggcggcguccggugagcucucgcuggcccuugaa
aauccgggggagaggguguaaaucucgcgccgggccguacccauauccgc
agcaggucuccaaggugaacagccucuggcauguuggaacaauguaggua
agggaagucggcaagccggauccguaacuucgggauaaggauuggcucua
agggcucagccgacuuagaacuggugcggaccaggggaauccgacuguuu
aauuaaaacaaagcaucgcgaaggcccgcggcggguguugacgcgaugug
auuucugcccagugcucugaaugucaaagugaagaaauucaaugaagcgc
ggguaaacggcgggaguaacuaugacucucuuaagguagccaaaugccuc
gucaucuaauuagugacgcgcaugaauggaugaacgagauucccacuguc
ccuaccuacuauccagcgaaaccacagccaagggaacgggcuuggcggaa
ucagcggggaaagaagacccuguugagcuugacucuagucuggcacggug
aagagacaugagagguguagaauaagugggaggccggccgccggugaaau
accacuacucugaucguuuuuucacugacccggugaggcgggggggcgag
ggcucucgcuucuggcgccaagcgcccggcgccgggcgcgacccgcuccg
gggacagugccagguggggaguuugacuggggcgguacaccugucaaacg
guaacgcagguguccuaaggcgagcucagggaggacagaaaccucccgug
gagcagaagggcaaaagcucgcuugaucuugauuuucaguacgaauacag
accgugaaagcggggccucacgauccuucugaccuuuuggguuuuaagca
ggaggugucagaaaaguuaccacagggauaacuggcuuguggcggccaag
cguucauagcgacgucgcuuuuugauccuucgaugucggcucuuccuauc
auugugaagcagaauucaccaagcguuggauuguucacccacuaauaggg
aacgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacu
gaugauguguuguugccaugguaauccugcucaguacgagaggaaccgca
gguucagacauuugguguaugugcuuggcugaggagccaauggggcgaag
cuaccaucugugggauuaugacugaacgccucuaagucagaaucccgccc
aggcggaacgauagccggagccucgguuggccucggauagccgguccccc
ggggguccggugcggagagcccuucgucccgggaaacggggcggccggaa
agggggccgcccucucgcccgucacgcaccgcacguucguggggaaccug
gcgcuaaaccauucguagacgaccugcuucugggucgggguuucguacgu
agcagagcaggcugcgaucuauugaaagucagcccucgacacaaggguuu
gu

.........................<<<<<<.....<......>......
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..>>>>...<<<<<<<<<....<<<<<<<<<<<<<<<<....<<<<....
..>>>>.....((......<<<.....))>>>...>.>>>>>>>...>>>
>>>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>....
.<<<<<..((((((>>>>>..>>>..........................
........<<....>>...............<<<<....>>>>.......
...........................<<<<<<<<..<<<.<....<<<<
<<<<.<<<.....<<<<<<<<<<....<<<<<<<.<<<<<.<<<<<<<.<
<<<<<<.....>>>>>.>>>>>>>>>>>>>>>>>>...>>>.>>>>>>>>
>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<<<...
<<..<<<..<<<<<<<......<<<<<<<...>>>>>>>.......>>>>
>>>..>>>.........>>>>>>..>>>>>>>>>>>.....<<.......
>>.........<...<<<<<.>>>>>....<<<...<<.<<<<<<<<<<<
<<<<<<<<<<<<.<<<<..>>>>.>>>>>>.>>>.>>>>>>>>>>>.>>>
...>>..>>>..........>.........>>.>>>>>....>>>>...>
>>>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..
<<<...<<<<.....>>>>.....>>>...<<....>>...<<<<<..>>
>>>..<<<<<...<<<..<<...[[..>>..>>>....>>>>>.<<<<<<
<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.
<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>
>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>
>...<<.......>>......>>>>>>......>.>>..<<<<<<....<
.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<<<...<<<....<
<<..<.<<<..<<<<.<.........>.>>>>>>>.>..>>>..>.>>..
.>>>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>
>>>>.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>
>...<<<<<<<...<<<(((.......<<<<<.....<<..........<
<<.<<<<<<<<<<.......<<<<<.<<<...................>>
>......................>>>>>...>>>>>>>>>>>>>......
....))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<.......
.............]]..........>>>>>>>>>....>>>>>>...<<<
<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..
......>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<.
....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<
<<...<<....<<<....>>>..........<<.<<<<<<<<<...>>>>
>>>>>>>......>>....>>>..<<<<<........>>>>>))<<<<..
..<<<<<..<<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<
<<<<...<<<<<<<<<<<<.<<<<<<<<<<..[[[..>>>>>>....<<<
<<.<<<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>..
...<<<<<<<.......>>>>>>>>>>>...>>>>>>>>>>>>.......
..>>>>>>>..>..........>>.>>>.>>>>.>>>..>>.....<<..
....>>>>>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<
.<<...............<<<<<....<<..>>.....>>>>><<.<<<<
<<<<<<.>>>>..>>>>>>.>>............<<<<<<<<<..<<<..
.......>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>.
..<<..<<<..<<<<<<....<<<.....<<<<....>>>>.....>>>.
>>>>..>>>>><<<<<<.......>>>>>>........<...........
<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>.....>
>.>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<
<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<
<<<<<<<<<.<<<<...........<<<<..<<<..>>>...........
.>>>>..>>>>.>>.>>>>>>>>>>...<<<<<.....<<<<..<<.<<.
...>>>>..............<<<<<<<<..>>>>>>>>..>>>>..>>>
>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<......
.....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>...
..<<...(((>>....>>>>>>>...<<<..<<<<.......>>>>..>>
><<<<....>>>>>>)))......<<<<<<<..<<<..)))))).>>>>>
>>>>>.........>>>>..>>>>>>.......<<.<.<.<<<<<<...<
<.......>>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<
.<.<<<<<......>>>>>...>.>.<<<.........>>>.....>>>>
>..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>
>>>.....<<<<<<.<..<<<..<.<<<<<.................>>>
>>.><<<<....<<<<....<<.<<<<<<<]]].>>>>>..>>.>>...>
>>>...>>>><<<<<...<<<<<...........>>>>>...>>>>>.>>
>...>.>>>>>>.<<<<<..<<<<....<<<........<<<<<..<<<<
.>>>>.>>>>>.......>>>...<<<..<<<<....<<<<<<<<<....
....>>>>>>.>>>...>>>>..>>>..............>>>>..>.>>
>>...<<<<<.<.............>....<<.<<<<<<<.<<.....<<
<<....<<<<.>>>>...>>>>....>>....>>>>.>>>.>>..>>>>>
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7tor Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): T2 N3 T4 K5 G6 K7 R8 R9 G10 R12 Y13 M14 S16 R17 F19 H22 G23 K35 D41 K43 G46 T47 Q49 K50 G51 H54 Y57 H58 G59 K60 T61 T68 Q69 H70 N77 K78 V80 K81 K87 R88 I89 N90 R92 K100 S101 R102 S104 F105 R108 V109 K110 W125 L128 K129 R130 F140 R142
• Binding residue (residue number reindexed from 1): T1 N2 T3 K4 G5 K6 R7 R8 G9 R11 Y12 M13 S15 R16 F18 H21 G22 K34 D40 K42 G45 T46 Q48 K49 G50 H53 Y56 H57 G58 K59 T60 T67 Q68 H69 N76 K77 V79 K80 K86 R87 I88 N89 R91 K99 S100 R101 S103 F104 R107 V108 K109 W124 L127 K128 R129 F139 R141

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7tor, PDBe:7tor, PDBj:7tor
• PDBsum: 7tor
• PubMed: 35589706
• UniProt: G1SHQ2|RL21_RABIT Large ribosomal subunit protein eL21 (Gene Name=RPL21)