Structure of PDB 7toq Chain AL21 Binding Site BS01

Receptor Information

>7toq Chain AL21 (length=159) Species: 9986 (Oryctolagus cuniculus)

TNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKG
MPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKS
RDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELL
EPIPYEFMA

Ligand information

>7toq Chain A25S (length=3649)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcgcgguccggccgugucggcg
gcccggcggaucucccgcucccgucggcgggggaccgucccccgaccggc
gaccggccgccgccgggcgcauuuccaccgcggcggugcgccgcgaccgg
cuccgggacggcugggaaggccccggcggggaagguggcccgggggcccc
ggguguuacagccccgccccggcagcagcagcucgccgaaucccggggcc
gagggagcgagacccgugcgccgcgcucuccccccucgggggggccgggc
caccccucccacggcgcgaccgcuggggcggacuguccagugcgccccgg
gcgggucgcgccgucgggcccggggggccacgcgucccccgcgagcgcac
ggggucggcggcgaugucggcuacccacccgacccgucuugaaacacgga
ccaaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccg
uggcgcaaugaaggugaaggccggcgcgcucgccggccgaggugggaucc
cgaggcaguccgccgagggcgcaccaccggcccgucucgcccgccgcgcc
ggggagguggagcacgagcgcacguguuaggacccgaaagauggugaacu
augccugggcagggcgaagccagaggaaacucugguggagguccguagcg
guccugacgugcaaaucggucguccgaccuggguauaggggcgaaagacu
aaucgaaccaucuaguagcugguucccuccgaaguuucccucaggauagc
uggcgcucuccgcaguuuuauccgguaaagcgaaugauuagaggucuugg
ggccgaaacgaucucaaccuauucucaaacuuuaaauggguaagaagccc
ggcucgcugguguggagccgggcguggaaugcgagugccuagugggccac
uuuugguaagcagaacuggcgcugcgggaugaaccgaacgccggguuaag
gcgcccgaugccgacgcucaucagaccccagaaaagguguugguugauau
agacagcaggaugguggccauggaagucggaauccgcuaaggagugugua
acaacucaccugccgaaucaacuagcccugaaaauggauggcgcuggagc
gucgggcccauacccggccgucgccggcagucggcagagcgagaaagaga
guggcgcggacgcuacgccgcgacgaguaggagggccgcugcggugagcc
uugaagccuagggcgcgggcccggguggagccgccgcaggugcagaucuu
ggugguaguagcaaauauucaaacgagaacuuugaaggccgaaguggaga
aggguuccaugugaacagcaguugaacaugggucagucgguccugagaga
ugggcgagcgccguuccgaagggacgggcgauggccuccguugcccucgg
ccgaucgaaagggagucggguucagauccccgaauccggaguggcggaga
ugggcgccgcgaggcguccagugcgguaacgcgaccgaucccggagaagc
cggcgggagccccggggagaguucucuuuucuuugugaagggcagggcgc
ccuggaauggguucgccccgagagaggggcccgugccuuggaaagcgucg
cgguuccggcggcguccggugagcucucgcuggcccuugaaaauccgggg
gagaggguguaaaucucgcgccgggccguacccauauccgcagcaggucu
ccaaggugaacagccucuggcauguuggaacaauguagguaagggaaguc
ggcaagccggauccguaacuucgggauaaggauuggcucuaagggcuggg
ucggucgcggccggcgccuagcagccgacuuagaacuggugcggaccagg
ggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcggcggg
uguugacgcgaugugauuucugcccagugcucugaaugucaaagugaaga
aauucaaugaagcgcggguaaacggcgggaguaacuaugacucucuuaag
guagccaaaugccucgucaucuaauuagugacgcgcaugaauggaugaac
gagauucccacugucccuaccuacuauccagcgaaaccacagccaaggga
acgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacuc
uagucuggcacggugaagagacaugagagguguagaauaagugggaggcc
ggccgccggugaaauaccacuacucugaucguuuuuucacugacccggug
aggcgggggggcgagccccgaggggcucucgcuucuggcgccaagcgccc
ggccgcgcgccggccgggcgcgacccgcuccggggacagugccagguggg
gaguuugacuggggcgguacaccugucaaacgguaacgcagguguccuaa
ggcgagcucagggaggacagaaaccucccguggagcagaagggcaaaagc
ucgcuugaucuugauuuucaguacgaauacagaccgugaaagcggggccu
cacgauccuucugaccuuuuggguuuuaagcaggaggugucagaaaaguu
accacagggauaacuggcuuguggcggccaagcguucauagcgacgucgc
uuuuugauccuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauacggca
gcgccggagccucgguuggccucggauagccggucccccgcgggguccgg
ugcggagagcccuucgucccgggaaacggggcggccggaaagggggccgc
ccucucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaacc
auucguagacgaccugcuucugggucgggguuucguacguagcagagcag
cucccucgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<<....<......>.....>
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..>>>>...<<<<<<<<<....<<<<<<<<.<......<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<.<<<.....<<<<<<<<<<....<<<<<<<.<<<<<.<
<....<<<<.<<<<<<<<<......>>>>>>>>>>>>>>>>>.>>>>>>>
...>>>.>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<<<<<
<<<<<<<<<<<<...<<..<<<..<<<<<<<......<<<<<<<<.>>>>
>>>>.......>>>>>>>..>>>.........>>>>>>..>>>>>>>>>>
>.....<<.......>>.........<...<<<<<<..>>>>>>....<<
<...<<.<<<<<<<<<<<<<<<<<<<<<<<.<<<<..>>>>.>>>>>>.>
>>.>>>>>>>>>>>.>>>...>>.>>.>................>.....
...>>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>><
<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>....
.>>>...<<....>>...<<<<<<<<....>>>>>>>>..<<<<<...<<
<..<<.{.[[..>>..>>>....>>>>>.<<<<<<<<<.<<<<......>
>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<...<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
..<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>
......>>>>>>......>.>>..<<<<<<....<.<<<....>>>.><<
<<<<<<<<...<<<.<<<<<<<<<...<<<....<<<..<.<<<..<<<<
.<.<.....>.>.>>>>>>>.>..>>>..>.>>...>>>>>>>...<<<<
<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<<<<.
<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<
<<(((.......<<<<<.....<<..........<<<.<<<<<<<<<<..
.....<<<<<.<<.....................>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>
>>>..>>>...>>>>>>><<<<<<.<<<....................]]
.}..............>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<.
.....<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>
>>><<<....<<.....<<<<<<.......>>>>>>.<<<.....<<<<<
.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<..
..<<<....>>>..........<<.<<<<<<<<<...>>>>>>>>>>>..
....>>....>>...<<<<<........>>>>>))<<<<....<<<<...
<<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<
<<<<<<<<<<<<<<<<<<<<<..{{{..>>>>>>.....<<<<.<<<<.<
<<<.....<<<.....>>>.....>>>>>>>>..>>>>......<<<<<<
<.......>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>>>>>>
..>..........>..>>>.>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<......
.........<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<..
.<<<<<..>>>>>........>>>>>..>>>>>>.>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>
>......>>>>>>>...<<..<<<..<<<<<<....<<<.....<<<<..
..>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<.......>>>>>.....>.....>>....>
>.>>>>>>>.....>>.>>>>>>>..>>>>>>...>>>....<<<<<...
.....>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<
<.<<<<<<<<<.<<<<<<<<<<<<.<<<<..........<<<<<..<<<.
.>>>............>>>>>.>>>>.>>.>>>>>>>>>>...<<<<<..
...<<<<..<<.<.<<<<....>>>>.>>>..............<<<<<<
<<<<<.....>>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>
>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<<...
<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>
>>>>>...<<<..<<<<.......>>>>..>>>.<<<....>>>.>>)))
.......((((((..(((..>>>>>>.)))))))))..........]]]]
..}}]]]].......<<.<...<<<<<<...<<.......>>...>>>>>
>....>.>.>.........<<<.<<<<<<..<.<.<<<<<......>>>>
>...>.>.<<<.<.....>.>>>.....>>>>>..>...>>>.......<
<<<<<<....>>>.>>>>....}}..}}}.]]]]].....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<<}}}.>>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>......
<<<<<<...<<<<....<<<........<<<<<..<<<<..>>>>.>>>>
>.......>>>...<<<..<<<<....<<<<<<<<<........>>>>>>
.>>>...>>>>..>>>..............>>>>....>>>>>>.<<<<<
.<.............>....<<.<<<<<<<.<<.....<<<<....<<<<
<......>>>>>...>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7toq Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): T2 N3 T4 K5 G6 K7 R8 R9 G10 R12 Y13 M14 S16 F19 H22 G23 K43 G46 T47 Q49 K50 G51 H54 Y57 H58 G59 K60 T61 T68 Q69 H70 N77 K78 Q79 V80 L85 K87 R88 I89 R92 K100 S101 R102 F105 R108 V109 K116 W125 K129 R130 F140 R142
• Binding residue (residue number reindexed from 1): T1 N2 T3 K4 G5 K6 R7 R8 G9 R11 Y12 M13 S15 F18 H21 G22 K42 G45 T46 Q48 K49 G50 H53 Y56 H57 G58 K59 T60 T67 Q68 H69 N76 K77 Q78 V79 L84 K86 R87 I88 R91 K99 S100 R101 F104 R107 V108 K115 W124 K128 R129 F139 R141

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7toq, PDBe:7toq, PDBj:7toq
• PDBsum: 7toq
• PubMed: 35589706
• UniProt: G1SHQ2|RL21_RABIT Large ribosomal subunit protein eL21 (Gene Name=RPL21)