Structure of PDB 7tor Chain AL11 Binding Site BS01

Receptor Information

>7tor Chain AL11 (length=170) Species: 9986 (Oryctolagus cuniculus)

KENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTV
RSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGF
GIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRI
SKEEAMRWFQQKYDGIILPG

Ligand information

>7tor Chain A28S (length=3552)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgcgggggcccaaguccuucuga
ucgaggcccagcccguggacggugugaggccgguagcggcccccggcgcg
ccgggcccgggucuucccggagucggguugcuugggaaugcagcccaaag
cgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauaguc
aacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaa
gagggcgugaaaccguuaagagguaaacgggugggguccgcgcaguccgc
ccggaggauucaacccggcggcgcgguccggccgugccggcggggcggau
ccccgcucucggcgggggaccgccccccggccggcgaccggccgccgccg
ggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacggcugg
gaaggccccggcggggaagguggcccgggggccccggguguuacagcccc
gccccggcagcagcagcucgccgaaucccggggccgagggagcgagaccc
gugcgccgcgcucuccccccggggggccgggccaccccucccacggcgcg
accgcuggggcggacuguccagugcgccccgggcgggucgcgccgucggg
cccggggggccacgcguccagcgcacggggucggcggcgaugucggcuac
ccacccgacccgucuugaaacacggaccaaggagucuaacacgugcgcga
gucgggggcuacgaaagccgccguggcgcaaugaaggugaaggccggccg
gccgaggugggaucccgaggcaguccgccgagggcgcaccaccggcccgu
cucgcccgccgcgccggggagguggagcacgagcgcacguguuaggaccc
gaaagauggugaacuaugccugggcagggcgaagccagaggaaacucugg
uggagguccguagcgguccugacgugcgaaucggucguccgaccugggua
uaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccaaagu
uucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagcgaau
gauuagaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaa
auggguaagaagcccggcucgcugguguggagccgggcguggaaugcgag
ugccuagugggccacuuuugguaagcagaacuggcgcugcgggaugaacc
gaacgccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaa
gguguugguugauauagacagcaggaugguggccauggaagucggaaucc
gcuaaggaguguguaacaacucaccugccgaaucaacuagcccugaaaau
ggauggcgcuggagcgucgggcccauacccggccgucgcuggcagucggc
agagcgagaaagagagggacgcuacgccgcgacgaguaggagggccgcug
cggugagccuugaagccuagggcgcgggcccggguggagccgccgcaggu
gcagaucuuggugguaguagcaaauauucaaacgagaacuuugaaggccg
aaguggagaaggguuccaugugaacagcaguugaacaugggucagucggu
ccugagagaugggcgagcgccguuccgaagggacgggcgauggccuccgu
ugcccucagccgaucgaaagggagucggguucagauccccgaauccggag
uggcggagaugggcgccgcgaggcguccagugcgguaacgcgaccgaucc
cggagaagccggcgggagccccggggagaguucucuuuucuuugugaagg
gcagggcgcccuggaauggguucgccccgagagaggggcccgugccuugg
aaagcgucgcgguuccggcggcguccggugagcucucgcuggcccuugaa
aauccgggggagaggguguaaaucucgcgccgggccguacccauauccgc
agcaggucuccaaggugaacagccucuggcauguuggaacaauguaggua
agggaagucggcaagccggauccguaacuucgggauaaggauuggcucua
agggcucagccgacuuagaacuggugcggaccaggggaauccgacuguuu
aauuaaaacaaagcaucgcgaaggcccgcggcggguguugacgcgaugug
auuucugcccagugcucugaaugucaaagugaagaaauucaaugaagcgc
ggguaaacggcgggaguaacuaugacucucuuaagguagccaaaugccuc
gucaucuaauuagugacgcgcaugaauggaugaacgagauucccacuguc
ccuaccuacuauccagcgaaaccacagccaagggaacgggcuuggcggaa
ucagcggggaaagaagacccuguugagcuugacucuagucuggcacggug
aagagacaugagagguguagaauaagugggaggccggccgccggugaaau
accacuacucugaucguuuuuucacugacccggugaggcgggggggcgag
ggcucucgcuucuggcgccaagcgcccggcgccgggcgcgacccgcuccg
gggacagugccagguggggaguuugacuggggcgguacaccugucaaacg
guaacgcagguguccuaaggcgagcucagggaggacagaaaccucccgug
gagcagaagggcaaaagcucgcuugaucuugauuuucaguacgaauacag
accgugaaagcggggccucacgauccuucugaccuuuuggguuuuaagca
ggaggugucagaaaaguuaccacagggauaacuggcuuguggcggccaag
cguucauagcgacgucgcuuuuugauccuucgaugucggcucuuccuauc
auugugaagcagaauucaccaagcguuggauuguucacccacuaauaggg
aacgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacu
gaugauguguuguugccaugguaauccugcucaguacgagaggaaccgca
gguucagacauuugguguaugugcuuggcugaggagccaauggggcgaag
cuaccaucugugggauuaugacugaacgccucuaagucagaaucccgccc
aggcggaacgauagccggagccucgguuggccucggauagccgguccccc
ggggguccggugcggagagcccuucgucccgggaaacggggcggccggaa
agggggccgcccucucgcccgucacgcaccgcacguucguggggaaccug
gcgcuaaaccauucguagacgaccugcuucugggucgggguuucguacgu
agcagagcaggcugcgaucuauugaaagucagcccucgacacaaggguuu
gu

.........................<<<<<<.....<......>......
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..>>>>...<<<<<<<<<....<<<<<<<<<<<<<<<<....<<<<....
..>>>>.....((......<<<.....))>>>...>.>>>>>>>...>>>
>>>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>....
.<<<<<..((((((>>>>>..>>>..........................
........<<....>>...............<<<<....>>>>.......
...........................<<<<<<<<..<<<.<....<<<<
<<<<.<<<.....<<<<<<<<<<....<<<<<<<.<<<<<.<<<<<<<.<
<<<<<<.....>>>>>.>>>>>>>>>>>>>>>>>>...>>>.>>>>>>>>
>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<<<...
<<..<<<..<<<<<<<......<<<<<<<...>>>>>>>.......>>>>
>>>..>>>.........>>>>>>..>>>>>>>>>>>.....<<.......
>>.........<...<<<<<.>>>>>....<<<...<<.<<<<<<<<<<<
<<<<<<<<<<<<.<<<<..>>>>.>>>>>>.>>>.>>>>>>>>>>>.>>>
...>>..>>>..........>.........>>.>>>>>....>>>>...>
>>>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..
<<<...<<<<.....>>>>.....>>>...<<....>>...<<<<<..>>
>>>..<<<<<...<<<..<<...[[..>>..>>>....>>>>>.<<<<<<
<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.
<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>
>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>
>...<<.......>>......>>>>>>......>.>>..<<<<<<....<
.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<<<...<<<....<
<<..<.<<<..<<<<.<.........>.>>>>>>>.>..>>>..>.>>..
.>>>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>
>>>>.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>
>...<<<<<<<...<<<(((.......<<<<<.....<<..........<
<<.<<<<<<<<<<.......<<<<<.<<<...................>>
>......................>>>>>...>>>>>>>>>>>>>......
....))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<.......
.............]]..........>>>>>>>>>....>>>>>>...<<<
<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..
......>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<.
....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<
<<...<<....<<<....>>>..........<<.<<<<<<<<<...>>>>
>>>>>>>......>>....>>>..<<<<<........>>>>>))<<<<..
..<<<<<..<<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<
<<<<...<<<<<<<<<<<<.<<<<<<<<<<..[[[..>>>>>>....<<<
<<.<<<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>..
...<<<<<<<.......>>>>>>>>>>>...>>>>>>>>>>>>.......
..>>>>>>>..>..........>>.>>>.>>>>.>>>..>>.....<<..
....>>>>>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<
.<<...............<<<<<....<<..>>.....>>>>><<.<<<<
<<<<<<.>>>>..>>>>>>.>>............<<<<<<<<<..<<<..
.......>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>.
..<<..<<<..<<<<<<....<<<.....<<<<....>>>>.....>>>.
>>>>..>>>>><<<<<<.......>>>>>>........<...........
<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>.....>
>.>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<
<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<
<<<<<<<<<.<<<<...........<<<<..<<<..>>>...........
.>>>>..>>>>.>>.>>>>>>>>>>...<<<<<.....<<<<..<<.<<.
...>>>>..............<<<<<<<<..>>>>>>>>..>>>>..>>>
>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<......
.....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>...
..<<...(((>>....>>>>>>>...<<<..<<<<.......>>>>..>>
><<<<....>>>>>>)))......<<<<<<<..<<<..)))))).>>>>>
>>>>>.........>>>>..>>>>>>.......<<.<.<.<<<<<<...<
<.......>>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<
.<.<<<<<......>>>>>...>.>.<<<.........>>>.....>>>>
>..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>
>>>.....<<<<<<.<..<<<..<.<<<<<.................>>>
>>.><<<<....<<<<....<<.<<<<<<<]]].>>>>>..>>.>>...>
>>>...>>>><<<<<...<<<<<...........>>>>>...>>>>>.>>
>...>.>>>>>>.<<<<<..<<<<....<<<........<<<<<..<<<<
.>>>>.>>>>>.......>>>...<<<..<<<<....<<<<<<<<<....
....>>>>>>.>>>...>>>>..>>>..............>>>>..>.>>
>>...<<<<<.<.............>....<<.<<<<<<<.<<.....<<
<<....<<<<.>>>>...>>>>....>>....>>>>.>>>.>>..>>>>>
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7tor Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): K19 C21 N23 C25 S51 A53 R54 Y55 F60 I68 R95 N97 N98 D101 T102 G106 F107 G108 G127 L128 D129 Y131 K145
• Binding residue (residue number reindexed from 1): K12 C14 N16 C18 S44 A46 R47 Y48 F53 I61 R88 N90 N91 D94 T95 G99 F100 G101 G120 L121 D122 Y124 K138

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7tor, PDBe:7tor, PDBj:7tor
• PDBsum: 7tor
• PubMed: 35589706
• UniProt: G1TUB8|RL11_RABIT Large ribosomal subunit protein uL5 (Gene Name=RPL11)