Structure of PDB 7toq Chain AL09 Binding Site BS01

Receptor Information

>7toq Chain AL09 (length=190) Species: 9986 (Oryctolagus cuniculus)

MKTILSNQTVDIPENVDISLKGRTVIVKGPRGTLRRDFNHINVELSLLGK
KKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFP
INVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELVLEGND
IELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQA

Ligand information

>7toq Chain A25S (length=3649)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcgcgguccggccgugucggcg
gcccggcggaucucccgcucccgucggcgggggaccgucccccgaccggc
gaccggccgccgccgggcgcauuuccaccgcggcggugcgccgcgaccgg
cuccgggacggcugggaaggccccggcggggaagguggcccgggggcccc
ggguguuacagccccgccccggcagcagcagcucgccgaaucccggggcc
gagggagcgagacccgugcgccgcgcucuccccccucgggggggccgggc
caccccucccacggcgcgaccgcuggggcggacuguccagugcgccccgg
gcgggucgcgccgucgggcccggggggccacgcgucccccgcgagcgcac
ggggucggcggcgaugucggcuacccacccgacccgucuugaaacacgga
ccaaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccg
uggcgcaaugaaggugaaggccggcgcgcucgccggccgaggugggaucc
cgaggcaguccgccgagggcgcaccaccggcccgucucgcccgccgcgcc
ggggagguggagcacgagcgcacguguuaggacccgaaagauggugaacu
augccugggcagggcgaagccagaggaaacucugguggagguccguagcg
guccugacgugcaaaucggucguccgaccuggguauaggggcgaaagacu
aaucgaaccaucuaguagcugguucccuccgaaguuucccucaggauagc
uggcgcucuccgcaguuuuauccgguaaagcgaaugauuagaggucuugg
ggccgaaacgaucucaaccuauucucaaacuuuaaauggguaagaagccc
ggcucgcugguguggagccgggcguggaaugcgagugccuagugggccac
uuuugguaagcagaacuggcgcugcgggaugaaccgaacgccggguuaag
gcgcccgaugccgacgcucaucagaccccagaaaagguguugguugauau
agacagcaggaugguggccauggaagucggaauccgcuaaggagugugua
acaacucaccugccgaaucaacuagcccugaaaauggauggcgcuggagc
gucgggcccauacccggccgucgccggcagucggcagagcgagaaagaga
guggcgcggacgcuacgccgcgacgaguaggagggccgcugcggugagcc
uugaagccuagggcgcgggcccggguggagccgccgcaggugcagaucuu
ggugguaguagcaaauauucaaacgagaacuuugaaggccgaaguggaga
aggguuccaugugaacagcaguugaacaugggucagucgguccugagaga
ugggcgagcgccguuccgaagggacgggcgauggccuccguugcccucgg
ccgaucgaaagggagucggguucagauccccgaauccggaguggcggaga
ugggcgccgcgaggcguccagugcgguaacgcgaccgaucccggagaagc
cggcgggagccccggggagaguucucuuuucuuugugaagggcagggcgc
ccuggaauggguucgccccgagagaggggcccgugccuuggaaagcgucg
cgguuccggcggcguccggugagcucucgcuggcccuugaaaauccgggg
gagaggguguaaaucucgcgccgggccguacccauauccgcagcaggucu
ccaaggugaacagccucuggcauguuggaacaauguagguaagggaaguc
ggcaagccggauccguaacuucgggauaaggauuggcucuaagggcuggg
ucggucgcggccggcgccuagcagccgacuuagaacuggugcggaccagg
ggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcggcggg
uguugacgcgaugugauuucugcccagugcucugaaugucaaagugaaga
aauucaaugaagcgcggguaaacggcgggaguaacuaugacucucuuaag
guagccaaaugccucgucaucuaauuagugacgcgcaugaauggaugaac
gagauucccacugucccuaccuacuauccagcgaaaccacagccaaggga
acgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacuc
uagucuggcacggugaagagacaugagagguguagaauaagugggaggcc
ggccgccggugaaauaccacuacucugaucguuuuuucacugacccggug
aggcgggggggcgagccccgaggggcucucgcuucuggcgccaagcgccc
ggccgcgcgccggccgggcgcgacccgcuccggggacagugccagguggg
gaguuugacuggggcgguacaccugucaaacgguaacgcagguguccuaa
ggcgagcucagggaggacagaaaccucccguggagcagaagggcaaaagc
ucgcuugaucuugauuuucaguacgaauacagaccgugaaagcggggccu
cacgauccuucugaccuuuuggguuuuaagcaggaggugucagaaaaguu
accacagggauaacuggcuuguggcggccaagcguucauagcgacgucgc
uuuuugauccuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauacggca
gcgccggagccucgguuggccucggauagccggucccccgcgggguccgg
ugcggagagcccuucgucccgggaaacggggcggccggaaagggggccgc
ccucucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaacc
auucguagacgaccugcuucugggucgggguuucguacguagcagagcag
cucccucgcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<<....<......>.....>
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>>>>>>..[[[...<<<<...<<<<<<<<<<<...>>>>>>>.>>>>...
..>>>>...<<<<<<<<<....<<<<<<<<.<......<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<.<<<.....<<<<<<<<<<....<<<<<<<.<<<<<.<
<....<<<<.<<<<<<<<<......>>>>>>>>>>>>>>>>>.>>>>>>>
...>>>.>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<<<<<
<<<<<<<<<<<<...<<..<<<..<<<<<<<......<<<<<<<<.>>>>
>>>>.......>>>>>>>..>>>.........>>>>>>..>>>>>>>>>>
>.....<<.......>>.........<...<<<<<<..>>>>>>....<<
<...<<.<<<<<<<<<<<<<<<<<<<<<<<.<<<<..>>>>.>>>>>>.>
>>.>>>>>>>>>>>.>>>...>>.>>.>................>.....
...>>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>><
<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>....
.>>>...<<....>>...<<<<<<<<....>>>>>>>>..<<<<<...<<
<..<<.{.[[..>>..>>>....>>>>>.<<<<<<<<<.<<<<......>
>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<...<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
..<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>
......>>>>>>......>.>>..<<<<<<....<.<<<....>>>.><<
<<<<<<<<...<<<.<<<<<<<<<...<<<....<<<..<.<<<..<<<<
.<.<.....>.>.>>>>>>>.>..>>>..>.>>...>>>>>>>...<<<<
<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<<<<.
<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<...<
<<(((.......<<<<<.....<<..........<<<.<<<<<<<<<<..
.....<<<<<.<<.....................>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>
>>>..>>>...>>>>>>><<<<<<.<<<....................]]
.}..............>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<.
.....<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>
>>><<<....<<.....<<<<<<.......>>>>>>.<<<.....<<<<<
.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<..
..<<<....>>>..........<<.<<<<<<<<<...>>>>>>>>>>>..
....>>....>>...<<<<<........>>>>>))<<<<....<<<<...
<<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<
<<<<<<<<<<<<<<<<<<<<<..{{{..>>>>>>.....<<<<.<<<<.<
<<<.....<<<.....>>>.....>>>>>>>>..>>>>......<<<<<<
<.......>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>>>>>>
..>..........>..>>>.>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<......
.........<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<..
.<<<<<..>>>>>........>>>>>..>>>>>>.>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>
>......>>>>>>>...<<..<<<..<<<<<<....<<<.....<<<<..
..>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<.......>>>>>.....>.....>>....>
>.>>>>>>>.....>>.>>>>>>>..>>>>>>...>>>....<<<<<...
.....>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<
<.<<<<<<<<<.<<<<<<<<<<<<.<<<<..........<<<<<..<<<.
.>>>............>>>>>.>>>>.>>.>>>>>>>>>>...<<<<<..
...<<<<..<<.<.<<<<....>>>>.>>>..............<<<<<<
<<<<<.....>>>>>>>>>>>..>>>>..>>>>>....>>>>>>>>>..>
>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<<...
<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>
>>>>>...<<<..<<<<.......>>>>..>>>.<<<....>>>.>>)))
.......((((((..(((..>>>>>>.)))))))))..........]]]]
..}}]]]].......<<.<...<<<<<<...<<.......>>...>>>>>
>....>.>.>.........<<<.<<<<<<..<.<.<<<<<......>>>>
>...>.>.<<<.<.....>.>>>.....>>>>>..>...>>>.......<
<<<<<<....>>>.>>>>....}}..}}}.]]]]].....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<<}}}.>>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>......
<<<<<<...<<<<....<<<........<<<<<..<<<<..>>>>.>>>>
>.......>>>...<<<..<<<<....<<<<<<<<<........>>>>>>
.>>>...>>>>..>>>..............>>>>....>>>>>>.<<<<<
.<.............>....<<.<<<<<<<.<<.....<<<<....<<<<
<......>>>>>...>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7toq Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): M1 R23 H40 I41 N42 V43 E44 K50 K53 R54 K59 W60 R64 K65 A68 R71 T72 S75 H76 H98 F99 E120 K121 Y122 R129 I151 S155 N156 Q163 K170 D171 R173 K174 K184
• Binding residue (residue number reindexed from 1): M1 R23 H40 I41 N42 V43 E44 K50 K53 R54 K59 W60 R64 K65 A68 R71 T72 S75 H76 H98 F99 E120 K121 Y122 R129 I151 S155 N156 Q163 K170 D171 R173 K174 K184

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7toq, PDBe:7toq, PDBj:7toq
• PDBsum: 7toq
• PubMed: 35589706
• UniProt: G1SWI6|RL9_RABIT Large ribosomal subunit protein uL6 (Gene Name=RPL9)