Structure of PDB 6p5k Chain AI Binding Site BS01

Receptor Information

>6p5k Chain AI (length=205) Species: 9986 (Oryctolagus cuniculus)

GRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGH
MVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINK
MLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVVEALRRAKFKF
PGRQKIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPNRGPLDKW
RALHS

Ligand information

>6p5k Chain 5 (length=3594)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcggguccggccgugccggcggggcgga
ucucccgcucccgucggcgggggaccgccccccggccggcgaccggccgc
cgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggacg
gcugggaaggcccggcggggaagguggcccgggggccccggguguuacag
cccacccggcagcagcagcucgccgaaucccggggccgagggagcgagac
cugugucgccgcgcucuccccccugggggggccgggccaccccucccacg
gcgcgaccgcuggggcggacuguccagugcgccccgggcgggucgcgccg
ucgggcccggggggccacgcgucccccgcgagcgcacggggucggcggcg
augucggcuacccacccgacccgucuugaaacacggaccaaggagucuaa
cacgugcgcgagucgggggcuacgaaagccgccguggcgcaaugaaggug
aaggccggcgccgccggccgaggugggaucccgaggccuuccaguccgcc
gagggcgcaccaccggcccgucucgcccgccgcgccggggagguggagca
cgagcgcacguguuaggacccgaaagauggugaacuaugccugggcaggg
cgaagccagaggaaacucugguggagguccguagcgguccugacgugcga
aucggucguccgaccuggguauaggggcgaaagacuaaucgaaccaucua
guagcugguucccuccaaaguuucccucaggauagcuggcgcucuccgca
guuuuauccgguaaagcgaaugauuagaggucuuggggccgaaacgaucu
caaccuauucucaaacuuuaaauggguaagaagcccggcucgcuggugug
gagccgggcguggaaugcgagugccuagugggccacuuuugguaagcaga
acuggcgcugcgggaugaaccgaacgccggguuaaggcgcccgaugccga
cgcucaucagaccccagaaaagguguugguugauauagacagcaggaugg
uggccauggaagucggaauccgcuaaggaguguguaacaacucaccugcc
gaaucaacuagcccugaaaauggauggcgcuggagcgucgggcccauacc
cggccgucgcuggcagucggcagagcgagaaagagaguggcgcggacgcu
acgccgcgacgaguaggagggccgcugcggugagccuugaagccuagggc
gcgggcccggguggagccgccgcaggugcagaucuuggugguaguagcaa
auauucaaacgagaacuuugaaggccgaaguggagaaggguuccauguga
acagcaguugaacaugggucagucgguccugagagaugggcgagcgccgu
uccgaagggacgggcgauggccuccguugcccucagccgaucgaaaggga
gucggguucagauccccgaauccggaguggcggagaugggcgccgcgagg
cguccagugcgguaacgcgaccgaucccggagaagccggcgggagccccg
gggagaguucucuuuucuuugugaagggcagggcgcccuggaauggguuc
gccccgagagaggggcccgugccuuggaaagcgucgcgguuccggcggcg
uccggugagcucucgcuggcccuugaaaauccgggggagaggguguaaau
cucgcgccgggccguacccauauccgcagcaggucuccaaggugaacagc
cucuggcauguuggaacaauguagguaagggaagucggcaagccggaucc
guaacuucgggauaaggauuggcucuaagggcuggcagccgacuuagaac
uggugcggaccaggggaauccgacuguuuaauuaaaacaaagcaucgcga
aggcccgcggcggguguugacgcgaugugauuucugcccagugcucugaa
ugucaaagugaagaaauucaaugaagcgcggguaaacggcgggaguaacu
augacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgc
augaauggaugaacgagauucccacugucccuaccuacuauccagcgaaa
ccacagccaagggaacgggcuuggcggaaucagcggggaaagaagacccu
guugagcuugacucuagucuggcacggugaagagacaugagagguguaga
auaagugggaggcccgggccgccggugaaauaccacuacucugaucguuu
uuucacugacccggugaggcgggggggcgagccgggcucucgcuucuggc
gccaagcgcccggccggccgggcgcgacccgcuccggggacagugccagg
uggggaguuugacuggggcgguacaccugucaaacgguaacgcagguguc
cuaaggcgagcucagggaggacagaaaccucccguggagcagaagggcaa
aagcucgcuugaucuugauuuucaguacgaauacagaccgugaaagcggg
gccucacgauccuucugaccuuuuggguuuuaagcaggaggugucagaaa
aguuaccacagggauaacuggcuuguggcggccaagcguucauagcgacg
ucgcuuuuugauccuucgaugucggcucuuccuaucauugugaagcagaa
uucaccaagcguuggauuguucacccacuaauagggaacgugagcugggu
uuagaccgucgugagacagguuaguuuuacccuacugaugauguguuguu
gccaugguaauccugcucaguacgagaggaaccgcagguucagacauuug
guguaugugcuuggcugaggagccaauggggcgaagcuaccaucuguggg
auuaugacugaacgccucuaagucagaaucccgcccaggcggaacgauag
cgccggagccucgguuggccucggauagccggucccccgccgggguccgg
ugcggagagcccuucgucccgggaaacggggcggccggaaagggggccgc
ccucucgcccgucacgcaccgcacguucguggggaaccuggcgcuaaacc
auucguagacgaccugcuucugggucgggguuucguacguagcagagcag
cugcugcgaucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>>..<<<<<<<<<....<<<<<<<.<<<<<<<<<<....<<<<..
....>>>>.....((......<<<.....))>>>...>.>>>>>>>..>>
>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>..
...<<<<<..((((((>>>>>..>>>........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<<.<....<<
<<<<<<.<<<.....<<<<<<<<<....<<<<<<<.<<<<<.<<<<<<<.
<<<<<<<<<......>>>>>>>>>>>>>>>>>>>>>>>>>...>>>..>>
>>>>>>>......>>>.>>>.>>>>>...<<<<<<<<<<<<<<<<<<.<<
<<...<<..<<<.....<<<......<<<<<<<...>>>>>>>.......
>>>....>>>.........>>>>>>...>>>>>>>>>>.....<<.....
{.>>......<<<<<<.<<<<<<..>>>>>>...<.<<..<<<.<<<<<<
<<<<<<<<<<<<<<<<<.<<<<..>>>>.>>>>>>>.>>>.>>>>>>>>>
>.>>>...>>>>>.>..............>>>>>>.....>>>.>>>>>.
...>>>>...>>>>>>>>..<<<<........>>>><<<<<<....<<<<
<<<<<<<<<..<<<...<<<<.....>>>>.....>>>...<<....>>.
..<<<<<<.....>>>>>>..<<<<<...<<<..<<<....[[.[[.>>>
..>>>....>>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>.
...>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<
....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.......
.>>>>.>>>....>>>>>>>>>...<<.......>>......>>>>>>..
.>..>.>>..<<<<<<....<.<<<....>>>.><<.<<<<<<<...<<<
.<<<<<<<<<...<<<....<<...<.<<<<..<<<.<.........>.>
>>>>>>.>...>>..>.>>...>>>>>>>...<<<<<<<<<.........
>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<<<<<.....>>>>
>.>>>>....<<<......>>>...<<<<<<<...<<<(((.......<<
<<<......<..........<<<.<<<<<<<<<<........<<<<.<..
...<<.........>>.....>.........<<......>>...>>>>..
..>>>>>>>>>>>>>..........))).>....>>>>>..>>>...>>>
>>>><<<<<<.<<<........}...........]].]]...........
..>>>>>>>>>....>>>>>>...<<<<<<<<.<<......<<<<.<<<<
....>>>>>>>>...>>>>>>>>>>........>>>>>><<.....<<..
...<<<<<<.......>>>>>>.<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
........<<.<<<<<.<<<...>>>.>>>>>>>......>>....>>>.
.<<<<<........>>>>>))<<<<....<<<<<...<<<<<<<....>>
>>>>>...<<<....>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<
<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<<<.....<<<...
..>>>.....>>>>>>>>..>>>>>.....<<<<<<<.......>>>>>>
>>>>>...>>>>>>>>>>>>.........>>>>>>>..>..........>
>.>>>.>>>>.>>>..>>.....<<......>>>>>>>.<<<<.....>>
>>..>>>....<<<<.....<<<<<<<.<<<........<.....<<<<<
...............>>>>><<.<<<<<<<<<<<.>>>>>..>>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....
<<<....<<<<<....>>>>.>...>>>.>>>>..>>>>>..<<<<....
...>>>>..........<...........<<<<<.......>>>>>....
.>.....>>....>>.>>>>>>>..>.>>>.>>>>>>>..>>>>>>....
>>....<<<<<........>>>>>....<<<<<..<<<.<<......<<<
<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<<<<<<.<.<<<<.......
...<<<.<..<<<<..>>>>............>.>>>.>>>>.>..>>>>
>>>>>>...<<<<<.....<<<<..<<.<<<.....>>>>>.........
.....<<<<<<<<<..>>>>>>>>>..>>>>..>>>>>....>>>>>>>>
>..>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<
<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>..
..>>>>>>>...<<<..<<<<.......>>>>..>>>.<<<....>>>.>
>)))].....<<<<<<<..<<<..)))))).>>>>>>>>>>.........
>>>>..>>>>>>.......<<.<...<<<<<<...<<.......>>...>
>>>>>....>.>.>.......<.<<<.<<<<<<..<.<.<<<<<......
>>>>>...>.>.<<<.........>>>.....>>>>>..>...>>>..>.
...<<<<<<<....>>>.>>>>....>>..>>>.>>>>>.....<<<<<<
.<..<<<..<.<<<<<.................>>>>>.><<<<....<<
<<...<<<.<<<<<<<]]].>>>>>..>>.>>>..>>>>...>>>><<<<
<...<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>.<
<<<<<...<<<<....<<<........<<<<<..<<<<<.>>>>>.>>>>
>.......>>>...<<<..<<<<....<<<<..<.............>..
>>>>...>>>>..>>>..............>>>>....>>>>>>.<<<<<
.<.............>....<<.<<<<<<<.<<.....<<<<.<..<<<<
<.>>>>>..>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6p5k The Israeli acute paralysis virus IRES captures host ribosomes by mimicking a ribosomal state with hybrid tRNAs.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): R3 R4 A6 R7 C8 Y9 K15 P16 Y17 F22 R24 D35 K39 K40 S61 E63 A64 A67 K74 R88 R90 H92 F94 R98 N100 G114 M115 R116 G117 A118 F119 G120 Q133 F157 K158 P160 R162 K175 D193 G194 C195
• Binding residue (residue number reindexed from 1): R2 R3 A5 R6 C7 Y8 K14 P15 Y16 F21 R23 D34 K38 K39 S60 E62 A63 A66 K73 R87 R89 H91 F93 R97 N99 G105 M106 R107 G108 A109 F110 G111 Q124 F148 K149 P151 R153 K166 D184 G185 C186

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation
• GO:0006417 regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6p5k, PDBe:6p5k, PDBj:6p5k
• PDBsum: 6p5k
• PubMed: 31609474
• UniProt: B7NZQ2|RL10_RABIT Large ribosomal subunit protein uL16 (Gene Name=RPL10)