Structure of PDB 8cqw Chain AF Binding Site BS01

Receptor Information

>8cqw Chain AF (length=124) Species: 5476 (Candida albicans)

ATSVPHPKIVKKYTKKFKRHHSDRYHRVAENWRKQKGIDSCVRRRFRGTI
PQPNIGYGSNKKTKFLNPAGYKVYLVKNVKDLDVLLLHTKSYAAEIASSV
SSRKRVEIVAKAKKLGVKVTNPKG

Ligand information

>8cqw Chain 1 (length=3208)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguauuuuugc
gguuuaccgggccagcaucgguuuggagcggcaggauaauggcggaggaa
uguggcacggcuucugcuguguguuauagccucugacgauacugccagcc
uagaccgaggacugcgguuaccuaggauguuggcauaaugaucuuaaguc
gcccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuug
gguguaaaacccguacgcguaaugaaagugaacgaaggugggggcccauu
agggugcaccaucgaccgauccugauguguucggauggauuugaguaaga
gcauagcuguugggacccgaaagauggugaacuaugccugaauaggguga
agccagaggaaacucugguggaggcucguagcgguucugacgugcaaauc
gaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagua
gcugguuccugccgaaguuucccucaggauagcagaagcucguaucaguu
uuaugagguaaagcgaaugauuagaagucuugggguugaaaugaccuuaa
cuuauucucaaacuuuaaauauguaagaaguccuuguugcuuaauugaac
guggacaauugaaugaagagcuuuuagugggccauuuuugguaagcagaa
cuggcgaugcgggaugaaccgaacgugaaguuaaagugccggaaugcacg
cucaucagacaccacaaaagguguuaguucaucuagacagccggacggug
gccauggaagucggaauccgcuaaggaguguguaacaacucaccggccga
augaacuagcccugaaaauggauggcgcucaagcgugcuacuuauacuuc
accgugauugcuguuuugacgcuuucacgaguaggcaggcguggagguca
gugacgaagccuuugcuguaaagcugggucgaacggccucuagugcagau
cuuggugguaguagcaaauauucaaaugagaacuuugaagacugaagugg
ggaaagguuccaugucaacagcaguuggacauggguuagucgauccuaag
agauggggaagcuccguuucaacgugcuugauuuuucaggccaaccaucg
aaagggaauccgguuaaaauuccggaacuuggauauggauucuucacggc
aacguaacugaauguggagacgucggcgugagcccugggaggaguuaucu
uuucuucuuaacagcuuaucacccuggaauugguuuauccggagaugggg
ucuuauggcuggaagagcgcgguaauuuugccgcguccggugcgcuuacg
acgguccuugaaaauccacaggaaggaauaguuuucaugccaagucguac
ucauaaccgcagcaggucuccaagguuaacagccucuaguugauagaaua
auguagauaagggaagucggcaaaauagauccguaacuucgggauaagga
uuggcucuaaggaucgguuuaacgaucaacuuagaacugguacggacaag
gggaaucugacugucuaauuaaaacauagcauugugauggucagaaagug
auguugacacaaugugauuucugcccagugcucugaaugucaaagugaag
aaauucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaa
gguagccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaa
cgagauucccacugucccuaucuacuaucuagcgaaaccacagccaaggg
aacgggcuuggcagaaucagcggggaaagaagacccuguugagcuugacu
cuaguuugacauugugaaaagacauggaggguguagaauaagugggagcu
ucggccggugaaaccacuaccucuauaguuuuuuuacuuauucaaugaag
cggagcuggaggucaaacuccacguucuagcauuaagcccucugggcgau
ccggguugaagacauugucagguggggaguuuggcuggggcggcacaucu
guuaaacgauaacgcagguguccuaagggggacucauggagaacagaaau
cuccaguagaacaaaaggguaaaaguccccuugauuuugauuuucagugu
gaauacaaaccaugaaaguguggccuaucgauccuuuagucccucggaau
uugaggcuagaggugccagaaaaguuaccacagggauaacuggcuugugg
cagucaagcguucauagcgacauugcuuuuugauucuucgaugucggcuc
uuccuaucauaccgaagcagaauucgguaagcguuggauuguucacccac
uaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuu
acccuacugaugaauguuaucgcaauaguaauugaacuuaguacgagagg
aaccguucauucagauaauugguuuuugcggcugucugaucaggcaacgc
cgcgaagcuaccaucugcuggauuauggcugaacgccucuaagucagaau
ccaugcuagaacgcgaugauuuuugcccugcacauuuuagauggauacga
auaagacuuuuuagucgcuggaccauagcaggcuggcaacggugcgcuua
gcggaaaggcuuugugcgcuugccggcggauagcaaugucaacaugcgcg
gggauaaauccuuugcauacgacuuagauguacaacggaguauuguaagc
aguagaguagccuuguuguuacgaucugcugagauuaagcucuuguuguc
ugauuugu

.......................<<<<<<.....<......>......>>
>>>.>.........<<....>>..<<<<<......<<.....>>.....>
>>>>..[[[...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<<....<<<<......>>>
>.(.......<<<......)>>>..>.>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
.<.<<....>>...>...........<<<<....>>>>............
......................<<<<<<<<<<<<.....<<<<..<....
.>..>>>>..<<<<<<<.<<<<<<<<.<<<<<<<....<<.<<<<<<<..
....<<<<<<......>>>>>>.......>>>>>>.>.>>.>>>>>.>>>
>>>>>>>........<<<.>>>.....>>>>>>>....>>>>>>>>>>>>
..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<..<<
<<<.....>>>>>..>>>...<<....>>.......<<<<<..<<<<...
.>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....
>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>>
>>>>>>....>>>>>>>>>...<<.......>>......>>>>>>...>.
.>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<
<<<<<.....<<<....<<<....<<<<...<<<<<<.....>>>>>>.>
>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>
>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.....>>>>>
.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<
<.....<<..........<<<.<<<<<<<<<<.......<<<<<.<<...
.<<.........>>....>>.........<<......>>...>>>>>...
>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>>
>><<<<<..<<.........>>..>>>>>....>>>>>>..<<<<<<<<.
<<......<<<<..<<<....>>>.>>>>...>>>>>>>>>>........
>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<<
<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<
<....<<<....>>>..........<<<<<<....>>>>>>......>>.
...>>...<<<<<........>>>>>))<<<<.<<<<<<<.....<<<..
..>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..{{{.
.>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>>
>.....>>>>>....><<<<<<<......>>>>>>>>>>>...>>>>>>>
.>>>>.........>>>>>>..........>>>..>>>>>>>>>>>>..>
>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<...
..<<<<<<<.<<...............<<<<<....<....>.....>>>
>><<.<<<<<<<<<<<......>>>>>..>>>>>>.>>............
<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>
>>......>>>>>>>...<<..<<<..<<<<<<....<<<.....<<<<.
...>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>>....
....<...........<<<<<.......>>>>>.....>.....>>....
>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<<..
......>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<
<<.<<<<<<<<..<<<<<<<<<<...<.<<....................
....................>>.>...>>>>>>>>>>...<<<<<.....
<<<<..<<<<<......>>>>>..............<<<<...>>>>..>
>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<
<<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<.......
>>>>.>>>>.<<<....>>>.>>))).......((((((..((((.>>>>
>>))))))))))..........]]]]..}}]]]].......<<.<...<<
<<<<...<<.......>>...>>>>>>....>.>.>.......<<<<<.<
<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}
..}}}.]]]]]....<<<<<<<<..<<<<...<<<<<.............
....>>>>>..<<<<<...<<<<....<<<<<<<<<<}}}.>>>>>..>>
>>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>
>>>.>>>>....>>>>>>>>.....<<<<<<.<<<.<.....<<......
....<<<......>>>......>>.......<<<<<<...<<<<<<<..<
<<......>>>..>>>>>>>.>>>>>>..............>.>>>.>>>
>>>..<<<<<<<.............>>....<<<<<<<<<.<<<....<<
<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>>.>>>>>.
.>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8cqw Drug-induced rotational movement of the ribosome is a key factor for read-through enhancement
• Resolution: 3.05 Å
• Binding residue (original residue number in PDB): A2 T3 K12 K13 Y14 T15 K16 F18 K19 R20 H22 D24 R25 Y26 H27 R28 V29 N32 W33 R34 K35 K37 G38 I39 D40 S41 C42 R44 R45 R46 F47 R48 G49 P52 P54 N55 I56 G57 Y58 G59 S60 N61 K62 K65 F66 L67 L76 K78 K81 E96 A98 S99 S100 S102 S103 R104 K105 N122 G125
• Binding residue (residue number reindexed from 1): A1 T2 K11 K12 Y13 T14 K15 F17 K18 R19 H21 D23 R24 Y25 H26 R27 V28 N31 W32 R33 K34 K36 G37 I38 D39 S40 C41 R43 R44 R45 F46 R47 G48 P51 P53 N54 I55 G56 Y57 G58 S59 N60 K61 K64 F65 L66 L75 K77 K80 E95 A97 S98 S99 S101 S102 R103 K104 N121 G124

External links

• PDB: RCSB:8cqw, PDBe:8cqw, PDBj:8cqw
• PDBsum: 8cqw
• UniProt: O94008|RL32_CANAX Large ribosomal subunit protein eL32 (Gene Name=RPL32)