Structure of PDB 8bqd Chain AF Binding Site BS01

Receptor Information

>8bqd Chain AF (length=81) Species: 4932 (Saccharomyces cerevisiae)

GKGTPSFGKRHNKSHTLCNRCGRRSFHVQKKTCSSCGYPAAKTRSYNWGA
KAKRRHTTGTGRMRYLKHVSRRFKNGFQTGS

Ligand information

>8bqd Chain BQ (length=3299)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacuggccagcaucaguuuugguggcaggauaauccauaggaaugua
gcuugccucgguaaguauuauagccugugggaauacugccagcugggacu
gaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccgcc
cgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugggu
guaaaacccauacgcguaaugaaagugaacguagguuggggccucgcaag
aggugcacaaucgaccgauccugaugucuucggauggauuugaguaagag
cauagcuguugggacccgaaagauggugaacuaugccugaauagggugaa
gccagaggaaacucugguggaggcucguagcgguucugacgugcaaaucg
aucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuaguag
ugguuccugccgaaguuucccucaggauagcagaagcucguaucaguuuu
augagguaaagcgaaugauuagagguuccggggucgaaaugaccuugacc
uauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaacgu
ggacauuugaaugaagagcuuuuagugggccauuuuugguaagcagaacu
ggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacacgcu
caucagacaccacaaaagguguuaguucaucuagacagccggacgguggc
cauggaagucggaauccgcuaaggaguguguaacaacucaccggccgaau
gaacuagcccugaaaauggauggcgcucaagcguguuaccuauacucuac
cgucaggguugauaugaugcccugacgaguaggcaggcguggaggucagu
gacgaagccuagaccguaaggucgggucgaacggccucuagugcagaucu
uggugguaguagcaaauauucaaaugagaacuuugaagacugaagugggg
aaagguuccacgucaacagcaguuggacguggguuagucgauccuaagag
auggggaagcuccguuucaaaggccugauuuuaugcaggccaccaucgaa
agggaauccgguuaagauuccggaaccuggauauggauucuucacgguaa
cguaacugaauguggagacgucggcgcgagcccugggaggaguuaucuuu
ucuucuuaacagcuuaucaccccggaauugguuuauccggagaugggguc
uuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugugac
ggcccgugaaaauccacaggaaggaauaguuuucaugccaggucguacug
auaaccgcagcaggucuccaaggugaacagccucuaguugauagaauaau
guagauaagggaagucggcaaaauagauccguaacuucgggauaaggauu
ggcucuaagggucggguagugagagccuuggucagacgcagcgggcgugc
uuguggacugcuugguggggcuugcucugcuaggcggacuacuugcgugc
cuuguuguagacggccuuggucuugcuacaauuaacgaucaacuuagaac
ugguacggacaaggggaaucugacugucuaauuaaaacauagcauugcga
uggucagaaagugauguugacgcaaugugauuucugcccagugcucugaa
ugucaaagugaagaaauucaaccaagcgcggguaaacggcgggaguaacu
augacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgc
augaauggauuaacgagauucccacugucccuaucuacuaucuagcgaaa
ccacagccaagggaacgggcuuggcagaaucagcggggaaagaagacccu
guugagcuugacucuaguuugacauugugaagagacauagagggcuaccu
uuauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuucca
cguucuagcauucaaggucccauucggggcugauccggguugaagacauu
gucagguggggaguuuggcuggggcggcacaucuguuaaacgauaacgca
gauguccuaaggggggcucauggagaacagaaaucuccaguagaacaaaa
ggguaaaagcccccuugauuuugauuuucagugugaauacaaaccaugaa
aguguggccuaucgauccuuuagucccucggaauuugaggcuagaggugc
cagaaaaguuaccacagggauaacuggcuuguggcagucaagcguucaua
gcgacauugcuuuuugauucuucgaugucggcucuuccuaucauaccgaa
gcagaauucgguaagcguuggauuguucacccacuaauagggaacgugag
cuggguuuagaccgucgugagacagguuaguuuuacccuacugaugaaug
uuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggau
aauugguuuuugcggcugucugaucaggcauugccgcgaagcuaccaucc
gcuggauuauggcugaacgccucuaagucagaauccaugcuagaacgcgg
ugauuucuuugcuccacacaauauagauggauacgaauaaggcguccuug
uggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaagg
ccuugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaau
cauuuguauacgacuuagauguacaacgggguauuguaagcaguagagua
gccuuguuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......>>
>>.(.......<<<......)>>>....>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.................
........<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<......
<<<<<<....>>>>>>.......>>>>>>>.>...>>>>>.>>>>>>>>>
>........<<<....>>>.....>>>>>>>.....>>>>>.>>>>>..<
<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<<<
.....>>>>...>>>...<<....>>.......<<<<<..<<<<<....>
>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....>
>>>>>.>>>>>>..<<....<<<<<<<.....<<<<<<<<<..<<<....
<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>>
>.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>..
.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<<<<
<<<.....<<<....<<<..<.<<<<...<<<<<<.....>>>>>>.>>>
>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.
>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>
>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.
....<<..........<<<.<<<<<<<<<<.......<<<<<.<<<...<
<.........>>...>>>.........<<......>>...>>>>>...>>
>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>>>>
<<<<<<<<<.........>>>>>>>>>....>>>>>><<<<<<<<<<.<<
......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>.>>.....>>
>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<<<<
<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<.
...<<<....>>>........<<<<<<........>>>>>>....>>...
.>>...<<<<<........>>>>>))<<<<.<<<<<<<.....<<<....
>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..{{{..>
>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>>>.
....>>>>>....><<<<<<<<....>>>>>>>>>>>>...>>>>>>>.>
>>>.........>>>>>>..........>>>..>>>>>>>>>>>>..>>.
....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....
<<<<<<<.<<.........<.....<<<<<...............>>>>>
<<.<<<<<<<<<<<..<<<<<......<..<<......<...<<<<.<.<
..<<<..<<<<..<<<<<<<....>>>>>>>..>>>>..>>>..>.>.>>
>.>...>......>>...>....>>>>>.......>>>>>..>>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....
.<<....<<<<<....>>>>.>...>>..>>>>..>>>>><<<<<<....
...>>>>>>........<...........<<<<<.......>>>>>....
.>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....
>>....<<<<<........>>>>>....[[[[[..{{{.{{......[[[
[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<<<<<<<......>>
>>>>.>>>>>>>>>>...<<<<<.....<<<<..<<<<........>>>>
...............<<<<<<....>>>>>>..>>>>..>>>>>.....>
>>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>
>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...(
((>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<...
.>>>.>>))).......((((((..((((.>>>>>>))))))))))....
......]]]]..}}]]]].......<<.<.<.<<<<<<...<<.......
>>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<
......>>>>>>>...>.<<<.........>>>.....>>>>>..>...>
>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]]....<
<<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<
...<<<<....<<<<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>>>
>.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>>>
>>>>.......<<<<<<.<<<.<<....<<..........<<<<<<....
.>>>>>>......>>.......<<<<<<...<<<<<.<..<<<......>
>>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<
<.<.............>.....<<<<<<<<<.<<<....<<<<.<..<<<
<........>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8bqd The dynamic architecture of Map1- and NatB-ribosome complexes coordinates the sequential modifications of nascent polypeptide chains.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): G2 K3 G4 T5 P6 S7 F8 G9 R11 H12 N13 K14 S15 H16 R24 R25 S26 H28 Q30 K31 S35 S36 K43 T44 R45 Y47 N48 W49 K52 K54 R55 R56
• Binding residue (residue number reindexed from 1): G1 K2 G3 T4 P5 S6 F7 G8 R10 H11 N12 K13 S14 H15 R23 R24 S25 H27 Q29 K30 S34 S35 K42 T43 R44 Y46 N47 W48 K51 K53 R54 R55

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:0044391 ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8bqd, PDBe:8bqd, PDBj:8bqd
• PDBsum: 8bqd
• PubMed: 37079644
• UniProt: P49166|RL37A_YEAST Large ribosomal subunit protein eL37A (Gene Name=RPL37A)