Structure of PDB 8q5i Chain AE Binding Site BS01

Receptor Information

>8q5i Chain AE (length=103) Species: 5476 (Candida albicans)

LQDVVTREYTINLHKRLHGVNFKKRAPKAVKEIKKFATLHMGTTDVRLDP
KLNIAIWKRGVQGVENRMRLRISRKRNAKEKLFAYVEPVIVPSTKGLQTV
VVE

Ligand information

>8q5i Chain 1 (length=3073)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguaccgggcc
agcaucgguuuggagcggcaggauaauggcggaggaauguggcacggcuu
cugcuguguguuauagccucugacgauacugccagccuagaccgaggacu
gcgguuuuuaccuaggauguuggcauaaugaucuuaagucgcccgucuug
aaacacggaccaaggagucuaacgucuaugcgaguguuuggguguaaaac
ccguacgcguaaugaaagugaacgaaggugggggcccauuagggugcacc
aucgaccgauccugauguguucggauggauuugaguaagagcauagcugu
ugggacccgaaagauggugaacuaugccugaauagggugaagccagagga
aacucugguggaggcucguagcgguucugacgugcaaaucgaucgucgaa
uuuggguauaggggcgaaagacuaaucgaaccaucuaguagcugguuccu
gccgaaguuucccucaggauagcagaagcucguaucaguuuuaugaggua
aagcgaaugauuagaagucuugggcuuaacuuauucucaaacuuuaaaua
uguaagaaguccuuguugcuuaauugaacguggacaauugaaugaagagc
uuuuagugggccauuuuugguaagcagaacuggcgaugcgggaugaaccg
aacgugaaguuaaagugccggaaugcacgcucaucagacaccacaaaagg
uguuaguucaucuagacagccggccgaaugaacuagcccugaaaauggau
ggcgcucaagcgugcuacuuauacuucaccgugauugcuguuuugacgcu
uucacgaguaggcaggcguggaggucagugacgaagccuuugcuguaaag
cugggucgaacggccucuagugcagaucuuggugguaguagcaaauauuc
aaaugagaacuuugaagacugaaguggggaaagguuccaugucaacagca
guuggacauggguuagucgauccuaagagauggggaagcuccguuucaac
gugcuugauuuuucaggccaaccaucgaaagggaauccgguuaaaauucc
ggaacuuggauauggauucuucacggcaacguaacugaauguggagacgu
cggcgugagcccugggaggaguuaucuuuucuucuuaacagcuuaucacc
cuggaauugguuuauccggagauggggucuuauggcuggaagagcgcggu
aauuuugccgcguccggugcgcuuacgacgguccuugaaaauccacagga
aggaauaguuuucaugccaagucguacucauaaccgcagcaggucuccaa
gguuaacagccucuaguugauagaauaauguagauaagggaagucggcaa
aauagauccguaacuucgggauaaggauuggcucuaaggaucggaacgau
caacuuagaacugguacggacaaggggaaucugacugucuaauuaaaaca
uagcauugugauggucagaaagugauguugacacaaugugauuucugccc
agugcucugaaugucaaagugaagaaauucaaccaagcgcggguaaacgg
cgggaguaacuaugacucucuuaagguagccaaaugccucgucaucuaau
uagugacgcgcaugaauggauuaacgagauucccacugucccuaucuacu
aucuagcgaaaccacagccaagggaacgggcuuggcagaaucagcgggga
aagaagacccuguugagcuugacucuaguuugacauugugaaaaguuuuu
acuuauucaaugaagcggagcuggaggucaaacuccacguucuagcauua
agcccucugggcgauccggguugaagacauugucagguggggaguuuggc
uggggcggcacaucuguuaaacgauaacgcagguguccuaagggggacuc
auggagaacagaaaucuccaguagaacaaaaggguaaaaguccccuugau
uuugauuuucagugugaauacaaaccaugaaaguguggccuaucgauccu
uuagucccucggaauuugaggcuagaggugccagaaaaguuaccacaggg
auaacuggcuuguggcagucaagcguucauagcgacauugcuuuuugauu
cuucgaugucggcucuuccuaucauaccgaagcagaauucgguaagcguu
ggauuguucacccacuaauagggaacgugagcuggguuuagaccgucgug
agacagguuaguuuuacccuacugaugaauguuaucgcaauaguaauuga
acuuaguacgagaggaaccguucauucagauaauugguuuuugcggcugu
cugaucaggcaacgccgcgaagcuaccaucugcuggauuauggcugaacg
ccucuaagucagaauccaugcuagaacgcgaugauuuuugcccugcacau
uuuagauggauacgaauaagacuuuuuagucgcuggaccauagcaggcug
gcaacggugcgcuuagcggaaaggcuuugugcgcuugccggcggauagca
augucaacaugcgcggggauaaauccuuugcauacgacuuagauguacaa
cggaguauuguaagcaguagaguagccuuguuguuacgaucugcugagau
uaagcucuuguugucugauuugu

.......................<<<<<<.....<......>......>>
>>>.>.........<<....>>..<<<<<......<<.....>>.....>
>>>>..[[[...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<.....<<<<......>>>
>.(.......<<<......)>>>....>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<<.....<<..>>..<<<
<<<<.<<<<<<<<.<<<<<<<....<<.<<<<<<<......<<<<<<...
...>>>>>>.......>>>>>>.>.>>.>>>>>.>>>>>>>>>>......
..<<<....>>>.....>>>>>>>....>>>>>>>>>>>>..<<<<....
....>>>><<<<<<....<<<<<<.<<<<<<..<<<..<<<<<.....>>
>>>..>>>...<<....>>.......<<<<<..<<<<....>>>>...>>
>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>
>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<..
..>>>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....
>>>>>>>>>...<<.......>>......>>>>>>...>..>.>>..<<<
<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<.....
<<<....<<<....<<<<...<<..>>.>>>>....>>>..>.>>.....
>>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>>.>>>>
>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>
...<<<<<<<..<<<<(((.....<<<<<<.....<<..........<<<
.<<<<<<<<<<.......<<..>>...>>>>>>>>>>>>>..........
))).>>...>>>>>>.>>>>..>>>>>>><<<<<..<<.........>>.
.>>>>>....>>>>>><.<<<<<<<<.<<......<<<<..<<<....>>
>.>>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<
<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<...
..>>.>>>>>>....((<<<<<<...<<....<<<....>>>........
..<<<<<<....>>>>>>......>>....>>...<<<<<........>>
>>>))<<<<.<<<<<<<.....<<<....>>>.......<<<<<<...<<
<<.<<<<<<<.<<<<<<<<<<..{{{..>>>>>><...<<<<<....<<<
<<<....<<<.....>>>....>>>>>>.....>>>>>....><<<<<<<
......>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>...
.......>>>..>>>>>>>>>>>>..>>.....<<......>>>>>>>.<
<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.........<.
....<<<<<...............>>>>><<.<<<<<<<<<<<...>>>>
>..>>>>>>.>>............<<<<<<<<<..<<<.........>>>
..<<<<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<.
.<<<<<<....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>
><<<<...........>>>>........<...........<<<<<.....
..>>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>.
.>>>>>>....>>....<<<<<........>>>>>....[[[[[..{{{.
{{......[[[[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<.>>>>>
>>...<<<<<.....<<<<..<<<<<......>>>>>.............
.<<<<...>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<
<.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<..
.<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<
<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>))).......((
((((..((((.>>>>>>))))))))))..........]]]]..}}]]]].
......<<.<.<.<<<<<<...<<.......>>...>>>>>>...>>.>.
>.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<
<<.........>>>.....>>>>>..>...>>>.>>....<<<<<<<...
.>>>.>>>>....}}..}}}.]]]]]....<<<<<<<<..<<<<...<<<
<<.................>>>>>..<<<<<...<<<<....<<<<<<<<
<<}}}.>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<.....
......>>>>>...>>>>.>>>>....>>>>>>>>.....<<<<<<.<<<
..<....<<..........<<<<....>>>>......>>.......<<<<
<<...<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>........
......>.>>>.>>>>>>..<<<<<<<.............>>....<<<<
<<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>
....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8q5i Structural characterization of cephaeline binding to the eukaryotic ribosome using Cryo-Electron Microscopy
• Resolution: 2.45 Å
• Binding residue (original residue number in PDB): R9 T12 N14 H16 K17 R18 H20 G21 V22 N23 F24 K25 R27 V32 K33 H42 L50 P52 N55 I56 W59 R61 V63 Q64 R69 G102 L103 Q104 T105
• Binding residue (residue number reindexed from 1): R7 T10 N12 H14 K15 R16 H18 G19 V20 N21 F22 K23 R25 V30 K31 H40 L48 P50 N53 I54 W57 R59 V61 Q62 R67 G96 L97 Q98 T99

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8q5i, PDBe:8q5i, PDBj:8q5i
• PDBsum: 8q5i
• UniProt: A0A8H6F1M1