Structure of PDB 3j9w Chain AE Binding Site BS01

Receptor Information

>3j9w Chain AE (length=165) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

RRIDPSKLELEERLVTVNRVAKVVKGGRRFRFAALVVVGDKNGHVGFGTG
KAQEVPEAIRKAVEDAKKNLIEVPMVGTTIPHEIIGRFGAGNILLKPASE
GTGVIAGGPVRAVLELAGVADILSKSLGSNTPINMIRATLQGLSELKRAE
DVAKLRGKSVEELLG

Ligand information

>3j9w Chain AA (length=1544)

ggagaguuugauccuggcucaggacgaacgcuggcggcgugccuaauaca
ugcaagucgagcggacagaugggagcuugcucccugauguuagcggcgga
cgggugaguaacacguggguaaccugccuguaagacugggauaacuccgg
gaaaccggggcuaauaccggaugguuguuugaaccgcaugguucaaacau
aaaagguggcuucggcuaccacuuacagauggacccgcggcgcauuagcu
aguuggugagguaacggcucaccaaggcgacgaugcguagccgaccugag
agggugaucggccacacugggacugagacacggcccagacuccuacggga
ggcagcaguagggaaucuuccgcaauggacgaaagucugacggagcaacg
ccgcgugagugaugaagguuuucggaucguaaagcucuguuguuagggaa
gaacaagugccguucgaauagggcgguaccuugacgguaccuaaccagaa
agccacggcuaacuacgugccagcagccgcgguaauacguagguggcaag
cguuguccggaauuauugggcguaaagggcucgcaggcgguuucuuaagu
cugaugugaaagcccccggcucaaccggggagggucauuggaaacugggg
aacuugagugcagaagaggagaguggaauuccacguguagcggugaaaug
cguagagauguggaggaacaccaguggcgaaggcgacucucuggucugua
acugacgcugaggagcgaaagcguggggagcgaacaggauuagauacccu
gguaguccacgccguaaacgaugagugcuaaguguuaggggguuuccgcc
ccuuagugcugcagcuaacgcauuaagcacuccgccuggggaguacgguc
gcaagacugaaacucaaaggaauugacgggggcccgcacaagcgguggag
caugugguuuaauucgaagcaacgcgaagaaccuuaccaggucuugacau
ccucugacaauccuagagauaggacguccccuucgggggcagagugacag
guggugcaugguugucgucagcucgugucgugagauguuggguuaagucc
cgcaacgagcgcaacccuugaucuuaguugccagcauucaguugggcacu
cuaaggugacugccggugacaaaccggaggaagguggggaugacgucaaa
ucaucaugccccuuaugaccugggcuacacacgugcuacaauggacagaa
caaagggcagcgaaaccgcgagguuaagccaaucccacaaaucuguucuc
aguucggaucgcagucugcaacucgacugcgugaagcuggaaucgcuagu
aaucgcggaucagcaugccgcggugaauacguucccgggccuuguacaca
ccgcccgucacaccacgagaguuuguaacacccgaagucggugagguaac
cuuuuaggagccagccgccgaaggugggacagaugauuggggugaagucg
uaacaagguagccguaucggaaggugcggcuggaucaccuccuu

...<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<.
<<<..<<<..<<.<<<<...<<<<<....>>>>>...>>>>.>>>>>...
...<<........<<<<<<<..<<...<<<<<<<.<<<<.....<<<<<<
....>>>>>>......>>>>.....<<<<<<<<<<....>>>>>>>>>>.
....<<<<<<....>>>>>>.>>>>>>>..>>>>>>>>>.<<<....<<<
..<<<<<<<<.......>>>>>>>>>>>......>>>..<<<<<<<<...
.>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>
>...>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>
>>>>..<<<<......<<<<....>>>>.....>>>>..<<<<<<<<...
....<<<<<<<<<<<.....>>>>>>>>>.>>.......>>>>>>...>>
.<<<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>
>>>>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<
<<<<<<.......<<<<<<.....>>>>>>.....>>>>>>>..>>>>>>
>>>...<<<.<<<<...<<<<<<<....<<<<<<<<...<<<......>>
>......>>>>>>>>...........<<....>>.>>>>>>>..>>>>..
>>>...>>>...>>>>....<<<<<<...<<...<<<<.........>>>
>...>>>>>>>>..........<<<<<<..<<<<<<<<<<<<<....>>>
>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<......<<<<
....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<
<<<<<<........<<........>>..........<<<<<<<......<
<<<<<<....<<<<<....>>>>>....<<<....>>>.>>>>>.>>.<<
<.<<<..<<<<<<.......<<<<<<<<<....>>>..<<<<......>>
>>..>>>>>>.....<<<<.<<<<<<<..<<<..<<......>>>>>...
>>>>>>>.....<<<<<.....>>>>>........>>>>.........>>
>...>>>>>>>>>...>>>>>>>......>>>>>>>>.....<<<<<<<.
....<<<..<<..<<<<....>>>>..>>....>>>.....>>>>>>>..
....<....<<<<<<<........>>>>>>>....>.....>>>>>>...
..<<<<<<.........>>>>>>.......>>...>>>>>>>>>>.>>..
..<..<<.<.<<<<.<<<..<<<<<<..<<<<....<.<<<<..<<<..<
<<...>>>.>>>.>>>>.>...>>>>..>>>>>>..>>>.>>>>..>.>>
...>.....<<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j9w Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): A22 V24 G27 G90 A107 S125 K126 S127
• Binding residue (residue number reindexed from 1): A21 V23 G26 G89 A106 S124 K125 S126

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j9w, PDBe:3j9w, PDBj:3j9w
• PDBsum: 3j9w
• PubMed: 25903689
• UniProt: P21467|RS5_BACSU Small ribosomal subunit protein uS5 (Gene Name=rpsE)