Structure of PDB 8c3a Chain AD Binding Site BS01

Receptor Information

>8c3a Chain AD (length=96) Species: 5476 (Candida albicans)

NINSKLALTIKSGKYTLGYKSVVKSLRTGKAKLVIIAANTPVLRKSELEY
YAMLSKTPVYYFQGGNNELGTVCGKLFRVGTLSILDAGDSDILSSI

Ligand information

>8c3a Chain 1 (length=3217)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguauuuugca
ugcgguuuaccgggccagcaucgguuuggagcggcaggauaauggcggag
gaauguggcacggcuucugcuguguguuauagccucugacgauacugcca
gccuagaccgaggacugcgguuuuuaccuaggauguuggcauaaugaucu
uaagucgcccgucuugaaacacggaccaaggagucuaacgucuaugcgag
uguuuggguguaaaacccguacgcguaaugaaagugaacgaagguggggg
cccauuagggugcaccaucgaccgauccugauguguucggauggauuuga
guaagagcauagcuguugggacccgaaagauggugaacuaugccugaaua
gggugaagccagaggaaacucugguggaggcucguagcgguucugacgug
caaaucgaucgucgaauuuggguauaggggcgaaagacuaaucgaaccau
cuaguagcugguuccugccgaaguuucccucaggauagcagaagcucgua
ucaguuuuaugagguaaagcgaaugauuagaagucuugggguugaaauga
ccuuaacuuauucucaaacuuuaaauauguaagaaguccuuguugcuuaa
uugaacguggacaauugaaugaagagcuuuuagugggccauuuuugguaa
gcagaacuggcgaugcgggaugaaccgaacgugaaguuaaagugccggaa
ugcacgcucaucagacaccacaaaagguguuaguucaucuagacagccgg
acgguggccauggaagucggaauccgcuaaggaguguguaacaacucacc
ggccgaaugaacuagcccugaaaauggauggcgcucaagcgugcuacuua
uacuucaccgugauugcuguuuugacgcuuucacgaguaggcaggcgugg
aggucagugacgaagccuuugcuguaaagcugggucgaacggccucuagu
gcagaucuuggugguaguagcaaauauucaaaugagaacuuugaagacug
aaguggggaaagguuccaugucaacagcaguuggacauggguuagucgau
ccuaagagauggggaagcuccguuucaacgugcuugauuuuucaggccaa
ccaucgaaagggaauccgguuaaaauuccggaacuuggauauggauucuu
cacggcaacguaacugaauguggagacgucggcgugagcccugggaggag
uuaucuuuucuucuuaacagcuuaucacccuggaauugguuuauccggag
auggggucuuauggcuggaagagcgcgguaauuuugccgcguccggugcg
cuuacgacgguccuugaaaauccacaggaaggaauaguuuucaugccaag
ucguacucauaaccgcagcaggucuccaagguuaacagccucuaguugau
agaauaauguagauaagggaagucggcaaaauagauccguaacuucggga
uaaggauuggcucuaaggaucgguuuaacgaucaacuuagaacugguacg
gacaaggggaaucugacugucuaauuaaaacauagcauugugauggucag
aaagugauguugacacaaugugauuucugcccagugcucugaaugucaaa
gugaagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacuc
ucuuaagguagccaaaugccucgucaucuaauuagugacgcgcaugaaug
gauuaacgagauucccacugucccuaucuacuaucuagcgaaaccacagc
caagggaacgggcuuggcagaaucagcggggaaagaagacccuguugagc
uugacucuaguuugacauugugaaaagacauggaggguguagaauaagug
ggagcuucggcgccggugaaauaccacuaccucuauaguuuuuuuacuua
uucaaugaagcggagcuggaggucaaacuccacguucuagcauuaagccc
ucugggcgauccggguugaagacauugucagguggggaguuuggcugggg
cggcacaucuguuaaacgauaacgcagguguccuaagggggacucaugga
gaacagaaaucuccaguagaacaaaaggguaaaaguccccuugauuuuga
uuuucagugugaauacaaaccaugaaaguguggccuaucgauccuuuagu
cccucggaauuugaggcuagaggugccagaaaaguuaccacagggauaac
uggcuuguggcagucaagcguucauagcgacauugcuuuuugauucuucg
augucggcucuuccuaucauaccgaagcagaauucgguaagcguuggauu
guucacccacuaauagggaacgugagcuggguuuagaccgucgugagaca
gguuaguuuuacccuacugaugaauguuaucgcaauaguaauugaacuua
guacgagaggaaccguucauucagauaauugguuuuugcggcugucugau
caggcaacgccgcgaagcuaccaucugcuggauuauggcugaacgccucu
aagucagaauccaugcuagaacgcgaugauuuugcccugcacauuuuaga
uggauacgaauaagacuuuuuagucgcuggaccauagcaggcuggcaacg
gugcgcuuagcggaaaggcuuugugcgcuugccggcggauagcaauguca
acaugcgcggggauaaauccuuugcauacgacuuagauguacaacggagu
auuguaagcaguagaguagccuuguuguuacgaucugcugagauuaagcu
cuuguugucugauuugu

.......................<<<<<<.....<......>......>>
>>>.>...{.....<<....>>..<<<<<......<<.....>>.....>
>>>>..[[[...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<<....<<<<......>>>
>.(.......<<<......)>>>..>.>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]....}..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<<.....<<<<..<<<<.
.>>>>..>>>>..<<<<<<<.<<<<<<<<.<<<<<<<....<<.<<<<<<
<......<<<<<<......>>>>>>.......>>>>>>.>.>>.>>>>>.
>>>>>>>>>>........<<<....>>>.....>>>>>>>....>>>>>>
>>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<
<<..<<<<<.....>>>>>..>>>...<<....>>.......<<<<<..<
<<<....>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.
>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..
<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<....
....>>>>.>>>....>>>>>>>>>...<<.......>>......>>>>>
>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<...
<<<.<<<<<<<.....<<<....<<<....<<<<...<<<<<.......>
>>>>.>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<....
..>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<....
.>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((....
.<<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<<<
.<<<...<<.........>>...>>>.........<<......>>...>>
>>>...>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>.
.>>>>>>><<<<<..<<.........>>..>>>>>....>>>>>>..<<<
<<<<<.<<......<<<<..<<<....>>>.>>>>...>>>>>>>>>>..
......>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.
....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<
<<...<<....<<<....>>>..........<<<<<<....>>>>>>...
...>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<....
.<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<
..{{{..>>>>>><...<<<<<....<<<<<<....<<<.....>>>...
.>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>>>...>
>>>>>>.>>>>.........>>>>>>..........>>>..>>>>>>>>>
>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<
<<<.....<<<<<<<.<<...............<<<<<............
...>>>>><<.<<<<<<<<<<<......>>>>>..>>>>>>.>>......
......<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.
....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<<....
.<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>
>>........<...........<<<<<.......>>>>>.....>.....
>>....>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<
<<<<........>>>>>....[[[[[..{{{.{{......[[[[.{{[[[
[..<<<<<.<<<<<<<<..<<<<<<<<<<...<.<<..............
..............................>>.>...>>>>>>>>>>...
<<<<<.....<<<<..<<<<<......>>>>>..............<<<<
...>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<....
.>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<
<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<
<<<.......>>>>.>>>>.<<<....>>>.>>))).......((((((.
.((((.>>>>>>))))))))))..........]]]]..}}]]]]......
.<<.<...<<<<<<...<<.......>>...>>>>>>....>.>.>....
...<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<<<...
......>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.
>>>>....}}..}}}.]]]]]....<<<<<<<<..<<<<...<<<<<...
..............>>>>>..<<<<<...<<<<....<<<<<<<<<<}}}
.>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<..........
.>>>>>...>>>>.>>>>....>>>>>>>>....<<<<<<.<<<.<....
.<<..........<<<......>>>......>>.......<<<<<<...<
<<<<<<..<<<......>>>..>>>>>>>.>>>>>>..............
>.>>>.>>>>>>..<<<<<<<.............>>....<<<<<<<<<.
<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>
>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c3a New crystal system to promote screening for new eukaryotic inhibitors
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): L27 G28 Y29 K30 S31 N49 P51 V52 L53 R54 K55 S56 E59 L86 F87 R88 V89 G90
• Binding residue (residue number reindexed from 1): L17 G18 Y19 K20 S21 N39 P41 V42 L43 R44 K45 S46 E49 L76 F77 R78 V79 G80

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0030627 pre-mRNA 5'-splice site binding

Biological Process

• GO:0006364 rRNA processing
• GO:0048025 negative regulation of mRNA splicing, via spliceosome

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8c3a, PDBe:8c3a, PDBj:8c3a
• PDBsum: 8c3a
• UniProt: A0A1D8PM75|RL30_CANAL Large ribosomal subunit protein eL30 (Gene Name=RPL30)