Structure of PDB 4w2i Chain AD Binding Site BS01

Receptor Information

>4w2i Chain AD (length=208) Species: 300852 (Thermus thermophilus HB8)

GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRP
SDYAVRLREKQKLRRIYGISERQFRNLFEEASKKKGVTGSVFLGLLESRL
DNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEK
SRNLELIRQNLEAMKGRKVGPWLSLDVEGMKGKFLRLPDREDLALPVNEQ
LVIEFYSR

Ligand information

>4w2i Chain AA (length=1496)

uuggagaguuugauccuggcucagggugaacgcuggcggcgugccuaaga
caugcaagucgugcgggccgcggguccguggucagcggcggacgggugag
uaacgcgugggugaccuacccggaagagggggacaacccggggacucggg
cuaaucccccauguggacccgccccuugggguguguccaaagggcuuugc
ccgcuuccggaugggcccgcgucccaucagcuaguuggugggguaauggc
ccaccaaggcgacgacggguagccggucugagaggauggccggccacagg
ggcacugagacacgggccccacuccuacgggaggcagcaguuaggaaucu
uccgcaaugggcgcaagccugacggagcgacgccgcuuggaggaagaagc
ccuucgggguguaaacuccugaacccgggacgaaacccccgacgagggga
cugacgguaccgggguaauagcgccggccaacuccgugccagcagccgcg
guaauacggagggcgcgagcguuacccggauucacugggcguaaagggcg
uguaggcggccuggggcgucccaugugaaagaccacggcucaaccguggg
ggagcgugggauacgcucaggcuagacggugggagagggugguggaauuc
ccggaguagcggugaaaugcgcagauaccgggaggaacgccgauggcgaa
ggcagccaccugguccacccgugacgcugaggcgcgaaagcguggggagc
aaaccggauuagauacccggguaguccacgcccuaaacgaugcgcgcuag
gucucugggucuccugggggccgaagcuaacgcguuaagcgcgccgccug
gggaguacggccgcaaggcugaaacucaaaggaauugacgggggcccgca
caagcgguggagcaugugguuuaauucgaagcaacgcgaagaaccuuacc
aggccuugacaugcuagggaacccgggugaaagccuggggugccccgcga
ggggagcccuagcacaggugcugcauggccgucgucagcucgugccguga
gguguuggguuaagucccgcaacgagcgcaacccccgccguuaguugcca
gcgguucggccgggcacucuaacgggacugcccgcgaaagcgggaggaag
gaggggacgacgucuggucagcauggcccuuacggccugggcgacacacg
ugcuacaaugcccacuacaaagcgaugccacccggcaacggggagcuaau
cgcaaaaaggugggcccaguucggauuggggucugcaacccgaccccaug
aagccggaaucgcuaguaaucgcggaucagccaugccgcggugaauacgu
ucccgggccuuguacacaccgcccgucacgccaugggagcgggcucuacc
cgaagucgccgggagccuacgggcaggcgccgaggguagggcccgugacu
ggggcgaagucguaacaagguagcuguaccggaaggugcggcugga

.....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<..<<
<.<<<..<<<..<<.<<<<<<<<..>>>>>>>>.>>>>>......<<...
....<<<<<<<<..<<...<<<<<<<.<<<<<....<<<<<....>>>>>
.....>>>>>...<<<<<.<<<<<....>>>>>.>>>>>..<<<<...>>
>>.>>>>>>>..>>>>>>>>>><<<....<<<..<<<<<<<<.......>
>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.<<
<<<.<.........>>>>>>.<<<<....>>>>...>>>>>>>.......
.<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<<<......<<
<<....>>>>.....>>>>....<<<<<........<<<<.....>>>>.
.........>>>>>......<<<<<(((...<<<<<.....<<.)))>>.
......>>>>>>>>>>..>>>>>>>>>..........<<<((.....<<<
<...<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....>>>>>>.
...>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<....<<<
<<<<.<..<<<......>>>.....>.>>>>>>>...........<<...
.>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<...<<
...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<..<
<<<<<<<<<...>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<
<<......<<<<....>>>>....>>>..)))).]<<<<<.<<<<<<<.<
<.<<<<<<..<<<<<<<<<<......<<........>>..........<<
<<<<<......<<<<<<<<..<<<<<<<....>>>>>>>.....<.....
.>....>>>>>>>>.<<<.<<<..<<<<<<.......<<<<<<<<<....
>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<<..<<<.
.<<<.....>>>>>>...>>>>>>>.....<<<<<....>>>>>......
..>>>>.........>>>...>>>>>>>>>...>>>>>>>...>>.>>>>
>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..>>....
>>>.....>>>>>>>......<..<.<<<<<<<........>>>>>>>>.
..>.....>>>>>>....<<<<<<<..........>>>>>>>......>>
...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<<<<<<<
<...<.<<<<....<<<....>>>.>>>>.>..>>>>>>>>>>>>>..>>
>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4w2i Negamycin Interferes with Decoding and Translocation by Simultaneous Interaction with rRNA and tRNA.
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): G2 R3 I5 P7 V8 C9 R10 R13 R14 K22 E24 R25 K30 R35 R36 Y38 P40 G41 Q42 H43 R49 S52 Y54 A55 R59 K61 Q62 R65 R66 E72 R73 Q74 N77 S113 R115 Q116 R118 Q119 R122 H123 H125 R132 V133 D134 L135 S137 Y138 R141 L155 E205 S208 R209
• Binding residue (residue number reindexed from 1): G1 R2 I4 P6 V7 C8 R9 R12 R13 K21 E23 R24 K29 R34 R35 Y37 P39 G40 Q41 H42 R48 S51 Y53 A54 R58 K60 Q61 R64 R65 E71 R72 Q73 N76 S112 R114 Q115 R117 Q118 R121 H122 H124 R131 V132 D133 L134 S136 Y137 R140 L154 E204 S207 R208

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4w2i, PDBe:4w2i, PDBj:4w2i
• PDBsum: 4w2i
• PubMed: 25306922
• UniProt: P80373|RS4_THET8 Small ribosomal subunit protein uS4 (Gene Name=rpsD)