Structure of PDB 4v66 Chain AB Binding Site BS01

Receptor Information

>4v66 Chain AB (length=218) Species: 562 (Escherichia coli)

MLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELN
KIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTNWKTVR
QSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLP
DALFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIR
AVTLYLGAVAATVREGRS

Ligand information

>4v66 Chain A1 (length=1530)

ugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaacac
augcaagucgaacgguaacaggaagaagcuugcuucuuugcugacgagug
gcggacgggugaguaaugucugggaaacugccugauggagggggauaacu
acuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagaggggg
accuucgggccucuugccaucggaugugcccagaugggauuagcuaguag
gugggguaacggcucaccuaggcgacgaucccuagcuggucugagaggau
gaccagccacacuggaacugagacacgguccagacuccuacgggaggcag
caguggggaauauugcacaaugggcgcaagccugaugcagccaugccgcg
uguaugaagaaggccuucggguuguaaaguacuuucagcggggaggaagg
gaguaaaguuaauaccuuugcucauugacguuacccgcagaagaagcacc
ggcuaacuccgugccagcagccgcgguaauacggagggugcaagcguuaa
ucggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaug
ugaaauccccgggcucaaccugggaacugcaucugauacuggcaagcuug
agucucguagagggggguagaauuccagguguagcggugaaaugcguaga
gaucuggaggaauaccgguggcgaaggcggcccccuggacgaagacugac
gcucaggugcgaaagcguggggagcaaacaggauuagauacccugguagu
ccacgccguaaacgaugucgacuuggagguugugcccuugaggcguggcu
uccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaaggu
uaaaacucaaaugaauugacgggggcccgcacaagcgguggagcaugugg
uuuaauucgaugcaacgcgaagaaccuuaccuggucuugacauccacgga
aguuuucagagaugagaaugugccuucgggaaccgugagacaggugcugc
auggcugucgucagcucguguugugaaauguuggguuaagucccgcaacg
agcgcaacccuuauccuuuguugccagcgguccggccgggaacucaaagg
agacugccagugauaaacuggaggaagguggggaugacgucaagucauca
uggcccuuacgaccagggcuacacacgugcuacaauggcgcauacaaaga
gaagcgaccucgcgagagcaagcggaccucauaaagugcgucguaguccg
gauuggagucugcaacucgacuccaugaagucggaaucgcuaguaaucgu
ggaucagaaugccacggugaauacguucccgggccuuguacacaccgccc
gucacaccaugggaguggguugcaaaagaaguagguagcuuaaccuucgg
gagggcgcuuaccacuuugugauucaugacuggggugaagucguaacaag
guaaccguaggggaaccugcgguuggauca

............<<<............<<<<...<<<.<<<......<<<
.<<<...<..........<<<<<<<<<<....>>>>>>>.>>>.......
>.......<..........<<<<<..<<...<<<<<<<.<.<<.....<<
<<<......>>>>>......>>.>.....<<<....>>>....<<<<<<.
.<<....>>>>>>>>.>>>>>>>..>>.>>>>>..<<<....<<<...<<
<<<.<........>>>>>>.>>>......>>>..<<<<<<........>>
...>>>>..>.<<<<.<<.........>>>>>>.<<<<....>>>>...>
>>>>>.........<<<.....<<......>>...>>>...>.>>.>>>.
.<<<<.......<........>......>>>>...<.<<<<<...<..<.
<<<<<<.<.<...>.>>>.>>>>.>>.......>>>>>....>..<<<<<
......<<<<......................>>>>>>>>>...>>>>..
.......................<.....<<<..<<<<<<.<<<<<<<<<
<......<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>....
<<<<<<<<....<<<<<<.....<<<<<<<...<..........>.....
.>>>>>>>.............<....>..>>>>>>...>>>>.>>>>...
>>>.....>.....<<<<.<...<<...<<<<.........>>>>...>>
.>>>>>............<<....<<<<<<<<<<<<.....>>>>>>>>>
>>>..................>>.................<<<....>>>
...........>>>.........<.<<<<.<<.<<<.<....<<<<.<..
.............................<<<<<<<......<<<<<<<.
.<<<<<<<....>>>>>>>...<<....>>..>>>>>.>>..<<......
<<<<<<.........<<<..............<<<......>>>....>>
>.......<<<..<<<<<<....<...<<<.....>>>.>.....>>>>>
>.....<<<<<.....>>>>>.........>>>.........>>>...>>
>...>>....>>>>>>>.......>.>>>>.......<<<<<<......<
<..<<...<<<....>>>...>>....>>......>>>>>>.........
...<<<<<<..........>>>>>>...........>.>>>.....<.<<
<<.........>>>>.>.......>>...>>>>..>..............
.....<....<....<<<<<<<<.......<<<<<<.<<<...<<....>
>..>>>>>>>>>......>>>>>>>>....>....>..............
.....<..............>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v66 The Structure of the E. coli Ribosome Before and After Accommodation: Implications for Proofreading
• Resolution: 9.0 Å
• Binding residue (original residue number in PDB): R20 P24 K25 K27 N35 K36 R73 R94 L96 G97 G98 T101 N102 K104 T105 Q108 S109 R112 K131
• Binding residue (residue number reindexed from 1): R13 P17 K18 K20 N28 K29 R66 R87 L89 G90 G91 T94 N95 K97 T98 Q101 S102 R105 K124

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0008270 zinc ion binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v66, PDBe:4v66, PDBj:4v66
• PDBsum: 4v66
• UniProt: P0A7V0|RS2_ECOLI Small ribosomal subunit protein uS2 (Gene Name=rpsB)