Structure of PDB 8btk Chain AA Binding Site BS01

Receptor Information

>8btk Chain AA (length=83) Species: 9986 (Oryctolagus cuniculus)

PLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQ
TVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH

Ligand information

>8btk Chain A2 (length=1770)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucagaucaaaaccaacccggugccggcggcuuugguga
cucuagauaaccucgggccgaucgcacgcccuccguggcggcgacgaccc
auucgaacgucugcccuaucaacuuucgaugguagucgccgugccuacca
uggugaccacgggugacggggaaucaggguucgauuccggagagggagcc
ugagaaacggcuaccacauccaaggaaggcagcaggcgcgcaaauuaccc
acucccgacccggggagguagugacgaaaaauaacaauacaggacucuuu
cgaggcccuguaauuggaaugaguccacuuuaaauccuuuaacgaggauc
cauuggagggcaagucuggugccagcagccgcgguaauuccagcuccaau
agcguauauuaaaguugcugcaguuaaaaagcucguaguuggaucuuggg
agcgggcggccgcccguccccgccucucggcgccccggggcccgaagcgu
uuacuuugaaaaaauuagaguguucaaagcaggcccgagccgccuggaua
ccgcagcuaggaauaauggaauaggaccgcgguucuauuuuguugguuuu
cggaacugaggccaugauuaagagggacggccgggggcauucguauugcg
ccgcuagaggugaaauucuuggaccggcgcaagacggaccagagcgaaag
cauuugccaagaauguuuucauuaaucaagaacgaaagucggagguucga
agacgaucagauaccgucguaguuccgaccauaaacgaugccgacuggcg
augcggcggcguuauucccaugacccgccgggcagcuuccgggaaaccaa
agucuuuggguuccggggggaguaugguugcaaagcugaaacuuaaagga
auugacggaagggcaccaccaggaguggagccugcggcuuaauuugacuc
aacacgggaaaccucacccggcccggacacggacaggauugacagauuga
uagcucuuucucgauuccgugggugguggugcauggccguucuuaguugg
uggagcgauuugucugguuaauuccgauaacgaacgagacucuggcaugc
uaacuaguuacgcgacccccgagcggucggcgucccccaacuucuuagag
ggacaaguggcguucagccacccgagauugagcaauaacaggucugugau
gcccuuagauguccggggcugcacgcgcgcuacacugacuggcucagcgu
gugccuacccuacgccggcaggcgcggguaacccguugaaccccauucgu
gauggggaucggggauugcaauuauuccccaugaacgaggaauucccagu
aagugcgggucauaagcuugcguugauuaagucccugcccuuuguacaca
ccgcccgucgcuacuaccgauuggaugguuuagugaggcccucggaucgg
ccccgccggggucggcccacggcccuggcggagcgcugagaagacggucg
aacuugacuaucuagaggaaguaaaagucguaacaagguuuccguaggug
aaccugcggaaggaucauua

...<<<<<...[[[[>>>>>[[[.((((((.....{{.........[[[.
[[[..<<....<<....<<..........>>...>>.>>......{{...
.....[[[......{{....[[[[[.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>>[[..(((((.....<<<<<<<<<<<<<..>>>>.......<....
..>..)))))......]]...<<<<.<<<<......>>>>>>>>.]].]]
]...}}....]]].<<<....<<<....<<<<<<<.........>>>>>>
>>>>......>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<.
.........>>>.>>.<.<<....>>.>...]]]]]].........<<<.
...<<<<...>>>>..>>>......}}.......<<<<<<<<<.<<<...
.>>>..>>>>>>.>>>......<<..<...........>..>>.......
..<<<<<((......<<<<.....<<..))>>.......>>>>.>>>>>.
.)))))).]]]............{{.......{{...[[[.[[....<<<
.<<<<<<<..>>>>>>>.>>><<...<<<..<<<<..>>>>.>>>.>>..
<<<<<<.<.......<<...<.......>.<<<<.......>>>>...>>
......>.>>>..>>>........{.[[.(((((((.....>>>>>>>>>
.))))))).]].}....]]....<<<<<<..<...<<<<..<<..<<<<<
<<<...<<<......>>>......>>>>>>>>..>>.......<<....>
>...>>>>..>..>>>.>>>...]]]...}}......<<<<<<<...<..
.<<<<.<.....>.>>>>...>>>>>>>>..........<<<.<<.<<<.
.<.<<<<<<.<<<<......>>>>>>>>>>.>..>>>...<<..}}>>..
.>>.....>>>.....<<<......<<<<....>>>>....>>>..]]]]
..<<<<<.<<<<<<<..<<..<<<<<..<<<.<<<<........<<....
....>>..........<<<<<......<<<<<<.......<<.<<<....
....>>>.>>.....>>>>>>...<<.<<<..<<<<<<<<<....<<<.<
<<<<....>>>...<<<......>>>...>>.>>>....<<<...<...<
<<<..<<<<<<<<<<<<.......>>>>.>>>>.....>>>>..>>>>..
>.....<<<<<.....>>>>>........>>>....>>>.>>>...>.>>
>>>>>.......>>>>>......>>>>.>>>.....<<.<<<........
<.......<<<.<<<<....>>>>.>>>....>.........>>>.>>..
....<.....<<<<<..........>>>>>.....>.....>>>>>....
.<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>.>>..
..<..<<.<..<<<<.<<....<<<<<<<<.<<<..<<<<.<<...<<<<
<<<<<<<<<<<<<<.....>>>>>>>>>>>>.>.>>>>>...>>.>>>>.
.>>>.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<..
..>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8btk Molecular basis of the TRAP complex function in ER protein biogenesis.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): R17 H19 K20 K21 K22 Q26 P28 S30 F47 H49 Q51 P66 T67 G68 G69 K70 R72 E75
• Binding residue (residue number reindexed from 1): R16 H18 K19 K20 K21 Q25 P27 S29 F46 H48 Q50 P65 T66 G67 G68 K69 R71 E74

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0046872 metal ion binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8btk, PDBe:8btk, PDBj:8btk
• PDBsum: 8btk
• PubMed: 37170030
• UniProt: G1TZ76|RS27_RABIT Small ribosomal subunit protein eS27 (Gene Name=RPS27)