Structure of PDB 8tzz Chain A Binding Site BS01

Receptor Information
>8tzz Chain A (length=1336) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIDLFLAAKNLSDAILLSDILR
VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKN
GYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDN
GSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFL
DNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYT
GWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKED
IQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIE
MARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDK
NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELD
KAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGD
YATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKK
YGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPID
FLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNELALPS
KYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRV
ILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTT
IDRKQYRSTKEVLDATLIHQSITGLYETRIDLSQLG
Ligand information
>8tzz Chain B (length=98) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gacgcauaaagaugagacgcguuuuagagcuagaaauagcaaguuaaaau
aaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuu
....................<<<<<<..<<<<....>>>>....>>>>>>
..<<.....>>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8tzz Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
Resolution3.56 Å
Binding residue
(original residue number in PDB)		K30 L35 I43 K44 N46 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 L101 S104 F105 E108 R115 H116 I122 E125 H129 I135 H160 K163 F164 G166 H167 Y325 H328 H329 K336 R340 F351 F352 I363 Q402 R403 T404 F405 N407 F446 R447 I448 Y450 G453 L455 A456 N459 S460 F462 W464 I473 F491 Y515 N588 R661 M694 S719 H721 S730 P731 K735 L738 K742 K749 R753 R765 S777 R778 R780 N803 K848 D850 S851 Y981 Y1010 Y1013 M1089 P1090 Q1091 E1099 V1100 T1102 G1103 G1104 F1105 E1108 I1110 P1112 K1113 R1122 K1124 Y1131 F1134 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356
Binding residue
(residue number reindexed from 1)		K28 L33 I41 K42 N44 E55 T56 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 L99 S102 F103 E106 R113 H114 I120 E123 H127 I133 H158 K161 F162 G164 H165 Y318 H321 H322 K329 R333 F344 F345 I356 Q395 R396 T397 F398 N400 F439 R440 I441 Y443 G446 L448 A449 N452 S453 F455 W457 I466 F484 Y508 N581 R654 M687 S707 H709 S718 P719 K723 L726 K730 K737 R741 R753 S765 R766 R768 N791 K836 D838 S839 Y969 Y998 Y1001 M1059 P1060 Q1061 E1069 V1070 T1072 G1073 G1074 F1075 E1078 I1080 P1082 K1083 R1092 K1094 Y1101 F1104 M1139 E1195 A1197 Q1242 H1319 Q1320 S1321 Y1326
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8tzz, PDBe:8tzz, PDBj:8tzz
PDBsum8tzz
PubMed38688943
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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